FastQCFastQC Report
Thu 26 May 2016
SRR1547309_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547309_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1449505
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT473693.2679431944008472No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTT17190.11859220906447375No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCT15880.10955464106712293No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT207.033896E-445.00000412
TAACGCG302.1658961E-645.0000041
ACTTACG406.8175723E-945.0000041
AGTATCG207.033896E-445.00000433
TAATTCG207.033896E-445.00000424
CGCTAGT302.1658961E-645.00000442
CGCTAGA302.1658961E-645.00000423
ACGGACG207.033896E-445.0000041
CCGAACG207.033896E-445.00000415
CGGTACG207.033896E-445.0000041
TAGTACG302.1658961E-645.0000041
TCGTTAG453.8562575E-1045.01
CGACGGT1100.045.028
CGTTTTT203200.044.1584631
CGCAGTA1300.043.2692337
GCGATCG850.042.352949
TCACGAC1200.041.25000425
CACGACG1200.041.25000426
CGCACGA603.6379788E-1241.25000439
GCCCTAC4500.041.026
Produced by FastQC (version 0.11.5)