##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547309_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1449505
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.270743460698654	31.0	31.0	33.0	30.0	34.0
2	31.654524820542186	31.0	31.0	34.0	30.0	34.0
3	31.52220102724723	31.0	31.0	34.0	30.0	34.0
4	35.413882670290896	37.0	35.0	37.0	33.0	37.0
5	35.37174690670263	37.0	35.0	37.0	33.0	37.0
6	35.43156801804754	37.0	35.0	37.0	33.0	37.0
7	35.919429046467584	37.0	35.0	37.0	35.0	37.0
8	35.939087481588544	37.0	35.0	37.0	35.0	37.0
9	37.68635016781591	39.0	37.0	39.0	35.0	39.0
10	36.90114832304821	39.0	37.0	39.0	32.0	39.0
11	36.65481043528653	39.0	35.0	39.0	32.0	39.0
12	36.304615023749484	38.0	35.0	39.0	32.0	39.0
13	36.11453358215391	38.0	35.0	39.0	31.0	39.0
14	37.270686199771646	39.0	35.0	41.0	32.0	41.0
15	37.430471781746185	39.0	35.0	41.0	32.0	41.0
16	37.522997850990514	39.0	35.0	41.0	32.0	41.0
17	37.44476355721436	39.0	35.0	41.0	32.0	41.0
18	37.20054846309602	39.0	36.0	40.0	32.0	41.0
19	36.95331440733216	38.0	35.0	40.0	32.0	41.0
20	36.630189616455276	38.0	35.0	40.0	32.0	41.0
21	36.496621260361295	38.0	35.0	40.0	31.0	41.0
22	36.395509501519484	38.0	35.0	40.0	31.0	41.0
23	36.301516034784285	38.0	35.0	40.0	31.0	41.0
24	36.13482188747193	37.0	35.0	40.0	31.0	41.0
25	36.01106170727248	37.0	35.0	40.0	31.0	41.0
26	35.763016340060915	37.0	35.0	40.0	30.0	41.0
27	35.667767962166394	36.0	34.0	40.0	30.0	41.0
28	35.57052649007765	36.0	34.0	40.0	30.0	41.0
29	35.49316904736445	36.0	34.0	40.0	30.0	41.0
30	35.35538614906468	36.0	34.0	40.0	29.0	41.0
31	35.12565530991614	36.0	34.0	40.0	27.0	41.0
32	34.73497849265784	36.0	34.0	40.0	25.0	41.0
33	34.28525876074936	36.0	33.0	40.0	23.0	41.0
34	33.800496031403824	36.0	33.0	40.0	21.0	41.0
35	33.444990531250326	36.0	33.0	40.0	18.0	41.0
36	33.103528445917746	35.0	33.0	40.0	16.0	41.0
37	33.000094515024095	35.0	33.0	40.0	15.0	41.0
38	32.919999586065586	35.0	32.0	40.0	15.0	41.0
39	32.78335086805496	35.0	32.0	40.0	15.0	41.0
40	32.67828810524972	35.0	32.0	40.0	15.0	41.0
41	32.65909534634237	35.0	32.0	40.0	15.0	41.0
42	32.55569659987375	35.0	31.0	40.0	13.0	41.0
43	32.44931407618463	35.0	31.0	40.0	12.0	41.0
44	32.35133787051441	35.0	31.0	40.0	12.0	41.0
45	32.21532454182635	35.0	31.0	40.0	10.0	41.0
46	32.11485990044877	35.0	31.0	40.0	10.0	41.0
47	32.07255304397018	35.0	31.0	39.0	10.0	41.0
48	31.93725168247091	35.0	31.0	39.0	10.0	41.0
49	31.79723146867379	35.0	30.0	39.0	10.0	41.0
50	31.660037047129883	35.0	30.0	39.0	10.0	41.0
51	31.2353913922339	35.0	29.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	0.0
12	2.0
13	6.0
14	22.0
15	67.0
16	187.0
17	523.0
18	1057.0
19	2158.0
20	3780.0
21	6089.0
22	9425.0
23	14062.0
24	21260.0
25	31956.0
26	43294.0
27	45779.0
28	42348.0
29	41279.0
30	43343.0
31	49293.0
32	58880.0
33	73627.0
34	110144.0
35	158464.0
36	141279.0
37	132807.0
38	169542.0
39	248742.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.072069430598724	23.543071600304934	26.799493620235875	22.585365348860474
2	39.14612229692205	23.9198209043777	25.322506648821495	11.611550149878752
3	24.45531405548791	23.562181572329866	39.68327118568063	12.2992331865016
4	21.704374941790476	25.565348170582368	39.64829372785882	13.081983159768335
5	20.086512292127313	29.115663623098918	38.58413734343793	12.213686741335836
6	18.371237077485073	37.505838199937216	35.279974887979	8.842949834598707
7	79.16792284262559	2.9832253079499553	16.53598987240472	1.3128619770197412
8	79.60600342875671	2.5539753226101323	16.239405866140512	1.6006153824926441
9	75.403879255332	4.586324296915153	17.76337439332738	2.2464220544254765
10	36.928261716930955	24.66793836516604	26.080075612019275	12.323724305883733
11	29.104625372109787	25.890838596624366	30.515796772001476	14.488739259264369
12	25.986940369298484	23.180741011586715	34.49280961431661	16.339509004798188
13	18.2033866733816	31.075436097150405	35.75758621046495	14.963591019003038
14	16.280868296418433	33.53110199688859	35.0730076819328	15.115022024760178
15	15.009054815264522	28.81638904315611	42.36804978251196	13.806506359067406
16	16.34882252907027	25.940096791663358	42.00716796423606	15.703912715030302
17	16.48342020206898	27.73381257739711	34.468870407483934	21.313896813049972
18	18.2180123559422	28.430050258536532	36.738403799917904	16.61353358560336
19	21.081058706247997	31.26136163724858	31.415483216684315	16.24209643981911
20	22.170672057012567	28.97554682460564	32.47729397277001	16.37648714561178
21	18.66982176674106	31.790921728452126	33.82389160437529	15.715364900431528
22	19.67526845371351	28.638259267819016	32.90585406742302	18.780618211044462
23	17.287349819421113	32.98463958385794	31.650459984615438	18.07755061210551
24	16.922604613299022	30.263986671311933	37.63553764905951	15.17787106632954
25	16.0374748621081	31.556289905864414	36.123435241685954	16.28279999034153
26	16.04051038113011	35.390150430664264	32.56670380578197	16.002635382423655
27	15.996288388104904	34.816851269916285	32.8805350792167	16.306325262762115
28	15.645892908268685	32.7066136370692	36.55378905212469	15.093704402537417
29	16.096460515831268	30.50462054287498	35.93888948296142	17.460029458332325
30	16.284869662401995	34.30046809083101	33.530343117133086	15.884319129633909
31	20.500791649563126	32.92682674430237	30.623971631694957	15.948409974439551
32	21.09382168395418	32.535313779531634	30.812518756403044	15.558345780111141
33	20.67581691680953	32.98553644175081	29.643498987585414	16.69514765385425
34	17.541160603102437	33.918613595675765	30.272472326759825	18.26775347446197
35	18.897071758979788	32.78277756889421	30.342565220540806	17.977585451585195
36	22.209995826161343	33.03472564772112	27.539125425576316	17.21615310054122
37	18.462854560694858	35.94827199630218	29.44998464993222	16.138888793070738
38	18.23677738262372	36.166139475200154	27.960027733605607	17.63705540857051
39	18.146677658924943	36.15765381975226	28.12256597942056	17.573102541902237
40	20.21117553923581	33.47791142493472	27.84122855733509	18.469684478494383
41	16.834643550729385	32.15608086898631	29.62556182972808	21.383713750556225
42	17.69341947768376	33.48708697106944	27.964787979344674	20.854705571902134
43	18.270444048140572	34.16207601905478	28.22535969175684	19.342120241047805
44	17.59731770500964	35.49501381506101	28.53105025508708	18.37661822484227
45	17.184556107084834	37.91328763957351	25.988527117878174	18.913629135463488
46	19.11894060386132	36.633126481109066	26.644130237563857	17.603802677465755
47	18.641674226718777	33.93682670980783	27.80445738372755	19.617041679745846
48	18.547987071448528	33.04079668576514	29.573613061010484	18.837603181775847
49	17.977516462516512	33.20554258177792	29.76864515817469	19.04829579753088
50	17.70376783798607	35.271213276256375	28.652126070624114	18.372892815133444
51	16.52674533720132	36.30749807692971	27.29290343945002	19.87285314641895
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1823.0
1	1945.0
2	2067.0
3	30618.5
4	59170.0
5	38153.5
6	17137.0
7	18783.5
8	20430.0
9	24788.5
10	29147.0
11	32967.5
12	36788.0
13	37490.5
14	38193.0
15	35257.5
16	32322.0
17	29259.0
18	26196.0
19	23481.5
20	20767.0
21	19920.0
22	19073.0
23	17425.0
24	15777.0
25	15773.0
26	16587.5
27	17406.0
28	19785.5
29	22165.0
30	24027.0
31	25889.0
32	29779.0
33	33669.0
34	36914.0
35	40159.0
36	43631.0
37	47103.0
38	54410.5
39	61718.0
40	68700.5
41	75683.0
42	83006.0
43	90329.0
44	96410.5
45	102492.0
46	106143.5
47	109795.0
48	107100.5
49	104406.0
50	96683.5
51	88961.0
52	80500.5
53	72040.0
54	62906.0
55	53772.0
56	49825.5
57	45879.0
58	40576.0
59	35273.0
60	31803.5
61	28334.0
62	25057.5
63	21781.0
64	17875.5
65	13970.0
66	11243.0
67	8516.0
68	7102.5
69	5689.0
70	4563.5
71	3438.0
72	2974.0
73	2510.0
74	2169.0
75	1434.5
76	1041.0
77	793.0
78	545.0
79	390.0
80	235.0
81	188.5
82	142.0
83	88.0
84	34.0
85	25.0
86	16.0
87	13.0
88	10.0
89	7.5
90	5.0
91	7.5
92	10.0
93	6.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1449505.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.41745794673287
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.41424899016053	25.095731611164368
2	7.575291118839203	4.911433625584577
3	3.6016344299685943	3.502674980190364
4	2.2759239071289454	2.951186701972662
5	1.4856498433211982	2.408049565971761
6	1.0357240270732215	2.014532405524019
7	0.7867940084606057	1.7854103177409149
8	0.5878667508002577	1.5245716537879876
9	0.468773542994336	1.3676801954903874
>10	3.858661455087918	26.015192781852868
>50	0.7276107726288087	16.49843540895594
>100	0.17903051040204015	7.859648233709231
>500	0.0015026539954140017	0.2921341584497846
>1k	0.0010733242824385726	0.4769622491698006
>5k	0.0	0.0
>10k+	2.1466485648771454E-4	3.296356110435408
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47369	3.2679431944008472	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTT	1719	0.11859220906447375	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCT	1588	0.10955464106712293	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.898906868206733E-5	0.0	0.0	0.03559835943994674	0.0
2	6.898906868206733E-5	0.0	0.0	0.13694330133390364	0.0
3	6.898906868206733E-5	0.0	0.0	0.2373223962663116	0.0
4	6.898906868206733E-5	0.0	0.0	0.38771856599321836	0.0
5	6.898906868206733E-5	0.0	0.0	0.7203838551781471	0.0
6	6.898906868206733E-5	0.0	0.0	1.4360764536859134	0.0
7	6.898906868206733E-5	0.0	0.0	1.902649525182735	0.0
8	6.898906868206733E-5	0.0	0.0	2.55556207118982	0.0
9	6.898906868206733E-5	0.0	0.0	2.930379681339492	0.0
10	6.898906868206733E-5	0.0	0.0	3.364320923349695	0.0
11	6.898906868206733E-5	0.0	0.0	3.747210254535169	0.0
12	6.898906868206733E-5	0.0	0.0	4.047243714233479	0.0
13	6.898906868206733E-5	0.0	0.0	4.25014056522744	0.0
14	6.898906868206733E-5	0.0	0.0	4.350450671091165	0.0
15	6.898906868206733E-5	0.0	0.0	4.438411733660802	0.0
16	6.898906868206733E-5	0.0	0.0	4.5802532588711315	0.0
17	6.898906868206733E-5	0.0	0.0	4.740997788900349	0.0
18	1.3797813736413466E-4	0.0	0.0	4.941204066215708	0.0
19	1.3797813736413466E-4	0.0	0.0	5.0626938161648285	0.0
20	1.3797813736413466E-4	0.0	0.0	5.190668538570064	0.0
21	1.3797813736413466E-4	0.0	0.0	5.352861839041604	0.0
22	1.3797813736413466E-4	0.0	0.0	5.521057188488484	0.0
23	1.3797813736413466E-4	0.0	0.0	5.7327156512050665	0.0
24	1.3797813736413466E-4	0.0	0.0	5.893046246822191	0.0
25	1.3797813736413466E-4	0.0	0.0	6.048616596700253	0.0
26	1.3797813736413466E-4	0.0	0.0	6.184318094797879	0.0
27	1.3797813736413466E-4	0.0	0.0	6.32381399167302	0.0
28	1.3797813736413466E-4	0.0	0.0	6.4697948610042735	0.0
29	1.3797813736413466E-4	0.0	0.0	6.629573544071942	0.0
30	1.3797813736413466E-4	0.0	0.0	6.8387484003159695	0.0
31	1.3797813736413466E-4	0.0	0.0	7.011428039227185	0.0
32	1.3797813736413466E-4	0.0	0.0	7.186315328336225	0.0
33	1.3797813736413466E-4	0.0	0.0	7.36803253524479	0.0
34	1.3797813736413466E-4	0.0	0.0	7.544299605727472	0.0
35	1.3797813736413466E-4	0.0	0.0	7.739331702891676	0.0
36	1.3797813736413466E-4	0.0	0.0	7.925464210195894	0.0
37	1.3797813736413466E-4	0.0	0.0	8.116908875788631	0.0
38	1.3797813736413466E-4	0.0	0.0	8.312699852708338	0.0
39	1.3797813736413466E-4	0.0	0.0	8.518701211792992	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	20	7.033896E-4	45.000004	12
TAACGCG	30	2.1658961E-6	45.000004	1
ACTTACG	40	6.8175723E-9	45.000004	1
AGTATCG	20	7.033896E-4	45.000004	33
TAATTCG	20	7.033896E-4	45.000004	24
CGCTAGT	30	2.1658961E-6	45.000004	42
CGCTAGA	30	2.1658961E-6	45.000004	23
ACGGACG	20	7.033896E-4	45.000004	1
CCGAACG	20	7.033896E-4	45.000004	15
CGGTACG	20	7.033896E-4	45.000004	1
TAGTACG	30	2.1658961E-6	45.000004	1
TCGTTAG	45	3.8562575E-10	45.0	1
CGACGGT	110	0.0	45.0	28
CGTTTTT	20320	0.0	44.158463	1
CGCAGTA	130	0.0	43.26923	37
GCGATCG	85	0.0	42.35294	9
TCACGAC	120	0.0	41.250004	25
CACGACG	120	0.0	41.250004	26
CGCACGA	60	3.6379788E-12	41.250004	39
GCCCTAC	450	0.0	41.0	26
>>END_MODULE