FastQCFastQC Report
Thu 26 May 2016
SRR1547308_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547308_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences897833
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140591.565881405562059No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC19430.2164099559717676No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9950.11082239124647901No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT302.1649685E-645.00000416
TTTAGCG750.045.0000041
AACGTAC302.1649685E-645.00000411
CTATGCG351.2115379E-745.0000041
TTGGCTA302.1649685E-645.00000422
CGGAACT207.03246E-445.00000413
GCCCATA207.03246E-445.00000427
TCGTTAA207.03246E-445.00000444
CGAACGT207.03246E-445.00000438
CTCGTTA207.03246E-445.00000443
TCACCGG207.03246E-445.0000042
GTCGATA351.2115379E-745.00000429
CTCAATC253.8901206E-545.00000427
GGTCGCT207.03246E-445.00000410
CGAGTAT207.03246E-445.00000442
CACGGGT502.1827873E-1145.0000044
ACGCACT253.8901206E-545.00000415
CTATAGT207.03246E-445.00000440
GACGTAC207.03246E-445.0000049
CGCATGG453.8380676E-1045.0000042