##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547308_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	897833
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.326246640522236	31.0	31.0	33.0	30.0	34.0
2	31.716491819748217	31.0	31.0	34.0	30.0	34.0
3	31.730368565200877	31.0	31.0	34.0	30.0	34.0
4	35.53212345725764	37.0	35.0	37.0	33.0	37.0
5	35.44524427148479	37.0	35.0	37.0	33.0	37.0
6	35.48702041470964	37.0	35.0	37.0	33.0	37.0
7	35.90010614446116	37.0	35.0	37.0	35.0	37.0
8	35.89776829321266	37.0	35.0	37.0	35.0	37.0
9	37.6732944768125	39.0	37.0	39.0	35.0	39.0
10	37.01708335514511	39.0	37.0	39.0	33.0	39.0
11	36.67567910736184	39.0	35.0	39.0	32.0	39.0
12	35.74620335853104	37.0	35.0	39.0	31.0	39.0
13	35.183081931717815	37.0	35.0	39.0	30.0	39.0
14	36.17980181169549	38.0	35.0	41.0	30.0	41.0
15	36.54376036523496	38.0	35.0	41.0	31.0	41.0
16	36.793415924787794	38.0	35.0	41.0	32.0	41.0
17	36.73410422650983	38.0	35.0	41.0	32.0	41.0
18	36.62061541511618	38.0	35.0	40.0	31.0	41.0
19	36.46483922956719	37.0	35.0	40.0	31.0	41.0
20	36.231153232282615	37.0	35.0	40.0	31.0	41.0
21	36.04120253989328	36.0	34.0	40.0	31.0	41.0
22	35.90807978766652	36.0	34.0	40.0	30.0	41.0
23	35.892544604620234	36.0	34.0	40.0	31.0	41.0
24	35.75206636423477	36.0	34.0	40.0	30.0	41.0
25	35.66128778960007	35.0	34.0	40.0	30.0	41.0
26	35.42099700055578	35.0	34.0	40.0	30.0	41.0
27	35.3561775965018	35.0	34.0	40.0	30.0	41.0
28	35.40960178563274	36.0	34.0	40.0	30.0	41.0
29	35.46441376068824	36.0	34.0	40.0	30.0	41.0
30	35.41075121988165	36.0	34.0	40.0	30.0	41.0
31	35.22797780879072	36.0	34.0	40.0	29.0	41.0
32	34.91368216583707	35.0	34.0	40.0	27.0	41.0
33	34.651536532963256	35.0	34.0	40.0	26.0	41.0
34	34.42423702403454	35.0	34.0	40.0	24.0	41.0
35	34.23509383148091	35.0	33.0	40.0	24.0	41.0
36	33.94210170488276	35.0	33.0	40.0	23.0	41.0
37	33.85536062942663	35.0	33.0	40.0	23.0	41.0
38	33.80068899227362	35.0	33.0	40.0	23.0	41.0
39	33.753709208728125	35.0	33.0	40.0	23.0	41.0
40	33.56736720525978	35.0	33.0	40.0	22.0	41.0
41	33.58560556361818	35.0	33.0	40.0	22.0	41.0
42	33.50935641706197	35.0	33.0	40.0	21.0	41.0
43	33.37873969880813	35.0	33.0	40.0	21.0	41.0
44	33.267656680028466	35.0	33.0	40.0	20.0	41.0
45	33.08572418255956	35.0	32.0	39.0	20.0	41.0
46	33.03217079345491	35.0	32.0	39.0	20.0	41.0
47	33.01086839089229	35.0	32.0	39.0	20.0	41.0
48	32.95196656839301	35.0	32.0	39.0	20.0	41.0
49	32.8733506119735	35.0	32.0	39.0	19.0	41.0
50	32.67706243811488	35.0	32.0	39.0	18.0	41.0
51	32.16996256542141	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	1.0
13	7.0
14	12.0
15	51.0
16	109.0
17	259.0
18	610.0
19	1106.0
20	1983.0
21	3117.0
22	4750.0
23	6871.0
24	10079.0
25	14469.0
26	19047.0
27	21768.0
28	22969.0
29	24726.0
30	27861.0
31	32891.0
32	40416.0
33	52363.0
34	89934.0
35	137295.0
36	66568.0
37	69530.0
38	96721.0
39	152260.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.781336841038364	24.44263019960282	28.0387332610853	16.737299698273507
2	32.66153059644722	26.154418472032106	28.8222865499486	12.361764381572074
3	26.576211834494835	25.72293511154079	34.43101333989729	13.269839714067091
4	23.572423824920673	28.87385516014671	33.20818014040473	14.34554087452789
5	21.502105625433682	31.798118358313854	32.697506106369445	14.002269909883019
6	19.858147339204507	40.433577291099795	30.19492489137735	9.513350478318351
7	85.01258029054401	3.7603875108177136	9.868650406033193	1.3583817926050836
8	85.49128846901372	3.413329650391554	9.084762979306841	2.010618901287879
9	82.31074152988363	4.476110813480903	10.825732625109568	2.387415031525907
10	48.81731903371785	26.5963714855658	15.24838138050172	9.337928100214627
11	43.43257599130351	21.056699853981755	23.21857182794573	12.29215232676901
12	40.31261938467399	21.509902175571625	24.730657037555982	13.446821402198406
13	21.0386564093768	40.26996111749067	25.477566540770948	13.213815932361586
14	15.014596255651105	43.092312267426124	26.393327044116223	15.499764432806545
15	13.605982404300132	25.820057850402023	47.09272214320481	13.48123760209304
16	15.656252332003836	21.092341226040922	47.25399935177255	15.997407090182694
17	15.545095802894302	22.185417555380564	29.056851329813004	33.21263531191212
18	19.71123805874812	25.247679690989305	35.469291059695955	19.57179119056662
19	26.293308443775175	27.171756885746014	27.140682064481926	19.394252605996883
20	29.784380836970797	25.009884911782038	26.949889344677686	18.255844906569486
21	20.948773324215082	31.771053191406416	29.72479291805937	17.555380566319126
22	21.998634489932982	26.41348669518719	26.87047591255835	24.717402902321478
23	18.534515884357113	32.76745229903557	25.239326244412936	23.458705572194383
24	18.66482965094845	25.53849101113459	39.65058089867492	16.14609843924204
25	16.40516666239713	26.5277618443519	37.01122591840576	20.05584557484521
26	15.729762661875874	35.6814686027357	29.320597483050857	19.268171252337574
27	16.61378006823095	36.79114044594039	28.625479348609375	17.96960013721928
28	14.511273254603028	31.353269483300345	38.24631083954365	15.889146422552969
29	15.458442717075446	25.951596789157893	37.61434476121951	20.975615732547144
30	16.661116265497036	33.421471476321315	32.633685774526	17.283726483655645
31	26.92661107355154	28.860155507761466	26.16455398721143	18.048679431475563
32	27.44107200336811	28.781410351368237	28.00643326765668	15.771084377606973
33	26.382746011786157	29.300326452692204	24.99707629369827	19.31985124182337
34	19.192878853862577	29.24441405027438	28.20390874472201	23.358798351141026
35	17.836056371285082	28.053101189196656	31.45696360013499	22.65387883938327
36	27.847272265554952	27.201383776270198	26.606841138608182	18.344502819566667
37	19.36172985399289	33.59689385442504	28.93210652760591	18.10926976397615
38	19.10622576804372	34.935227375246846	25.107787305657066	20.850759551052366
39	19.111571973852598	33.70793900424689	27.36243822626257	19.818050795637944
40	23.47953349899146	27.892492256355023	26.471849441934083	22.156124802719436
41	16.284320135259005	26.840292125595738	29.00695340893017	27.868434330215088
42	19.947362148640117	27.321450648394524	26.370048772990078	26.361138429975284
43	20.410477226833944	28.6223607285542	26.57688011022094	24.390281934390917
44	18.966778899862224	31.639737011225915	28.10934772947753	21.284136359434328
45	16.823730025516994	37.18720519294791	23.689372076989816	22.299692704545276
46	20.866464030615937	33.84493552809932	26.23516845560366	19.05343198568108
47	19.591950841637587	29.01085168399914	27.803834343357842	23.593363131005432
48	21.3035163554915	26.247977073687423	31.54094358304941	20.90756298777167
49	20.48833134892569	25.585270311962248	31.87497006681643	22.051428272295627
50	18.24325904706109	32.89832296206533	28.23709977245212	20.621318218421468
51	17.459482999622423	33.67129521859856	26.01463746598755	22.854584315791467
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1305.0
1	1456.0
2	1607.0
3	9935.5
4	18264.0
5	11929.5
6	5595.0
7	6132.5
8	6670.0
9	8084.0
10	9498.0
11	10145.5
12	10793.0
13	10742.0
14	10691.0
15	9677.5
16	8664.0
17	7834.5
18	7005.0
19	6441.5
20	5878.0
21	6048.0
22	6218.0
23	5706.0
24	5194.0
25	6097.5
26	7164.0
27	7327.0
28	9036.5
29	10746.0
30	11927.5
31	13109.0
32	14773.0
33	16437.0
34	19185.5
35	21934.0
36	23443.5
37	24953.0
38	28777.5
39	32602.0
40	45151.0
41	57700.0
42	69067.5
43	80435.0
44	82829.5
45	85224.0
46	86169.5
47	87115.0
48	83139.5
49	79164.0
50	71875.5
51	64587.0
52	58076.0
53	51565.0
54	43062.0
55	34559.0
56	32796.5
57	31034.0
58	28777.5
59	26521.0
60	23764.5
61	21008.0
62	18810.5
63	16613.0
64	14049.5
65	11486.0
66	8970.5
67	6455.0
68	5578.0
69	4701.0
70	3934.0
71	3167.0
72	2707.0
73	2247.0
74	1719.0
75	971.5
76	752.0
77	584.5
78	417.0
79	341.5
80	266.0
81	170.0
82	74.0
83	49.0
84	24.0
85	22.0
86	20.0
87	13.5
88	7.0
89	5.5
90	4.0
91	3.5
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	897833.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.575580751612616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.36210156993235	20.240964780043928
2	7.023024495879884	3.451898112380991
3	2.3836559197349687	1.7573918561851847
4	1.2387879434612854	1.2177573255462777
5	0.7499860162875076	0.9215670952926948
6	0.5239622975172208	0.7726006652060962
7	0.400659457819445	0.6892507201677369
8	0.30876183634241866	0.6070401153639446
9	0.23426606431067365	0.5181502122746491
>10	2.6802102176940625	15.772186012252803
>50	1.233634089669608	22.325300747480696
>100	0.8577647580315989	29.544636610670782
>500	0.002275238084964453	0.3917425727870356
>1k	4.550476169928906E-4	0.21728684525412797
>5k	0.0	0.0
>10k+	4.550476169928906E-4	1.5722263290930443
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14059	1.565881405562059	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1943	0.2164099559717676	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	995	0.11082239124647901	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1137928768490353E-4	0.0	0.0	0.05401895452717822	0.0
2	1.1137928768490353E-4	0.0	0.0	0.1988120285175528	0.0
3	1.1137928768490353E-4	0.0	0.0	0.2943754573512001	0.0
4	1.1137928768490353E-4	0.0	0.0	0.5693709186452269	0.0
5	1.1137928768490353E-4	0.0	0.0	1.0261373774410163	0.0
6	1.1137928768490353E-4	0.0	0.0	1.5382593422162028	0.0
7	1.1137928768490353E-4	0.0	0.0	1.838203763951648	0.0
8	1.1137928768490353E-4	0.0	0.0	2.428402609393952	0.0
9	1.1137928768490353E-4	0.0	0.0	2.6408029110090627	0.0
10	1.1137928768490353E-4	0.0	0.0	3.0693904100205716	0.0
11	1.1137928768490353E-4	0.0	0.0	3.5431978998321516	0.0
12	1.1137928768490353E-4	0.0	0.0	3.9667733308978397	0.0
13	1.1137928768490353E-4	0.0	0.0	4.123261230095129	0.0
14	1.1137928768490353E-4	0.0	0.0	4.182849149006552	0.0
15	1.1137928768490353E-4	0.0	0.0	4.273066372031324	0.0
16	1.1137928768490353E-4	0.0	0.0	4.4590697824651135	0.0
17	1.1137928768490353E-4	0.0	0.0	4.657324914544241	0.0
18	1.1137928768490353E-4	0.0	0.0	4.908707966849069	0.0
19	1.1137928768490353E-4	0.0	0.0	5.054948971579347	0.0
20	1.1137928768490353E-4	0.0	0.0	5.1967348048022295	0.0
21	1.1137928768490353E-4	0.0	0.0	5.3992223498133844	0.0
22	1.1137928768490353E-4	0.0	0.0	5.588455759590035	0.0
23	1.1137928768490353E-4	0.0	0.0	5.8000764061913515	0.0
24	1.1137928768490353E-4	0.0	0.0	5.971823267801473	0.0
25	1.1137928768490353E-4	0.0	0.0	6.1079287573524255	0.0
26	1.1137928768490353E-4	0.0	0.0	6.246261832657075	0.0
27	1.1137928768490353E-4	0.0	0.0	6.365771808342977	0.0
28	1.1137928768490353E-4	0.0	0.0	6.490627989837754	0.0
29	1.1137928768490353E-4	0.0	0.0	6.632302443772951	0.0
30	1.1137928768490353E-4	0.0	0.0	6.812514131247125	0.0
31	1.1137928768490353E-4	0.0	0.0	6.975796166993193	0.0
32	1.1137928768490353E-4	0.0	0.0	7.125935446792443	0.0
33	1.1137928768490353E-4	0.0	0.0	7.271619555084297	0.0
34	1.1137928768490353E-4	0.0	0.0	7.420199524855959	0.0
35	1.1137928768490353E-4	0.0	0.0	7.621907414853319	0.0
36	1.1137928768490353E-4	0.0	0.0	7.785189450599388	0.0
37	1.1137928768490353E-4	0.0	0.0	7.945241487002594	0.0
38	1.1137928768490353E-4	0.0	0.0	8.107075592008758	0.0
39	1.1137928768490353E-4	0.0	0.0	8.263006594767624	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	30	2.1649685E-6	45.000004	16
TTTAGCG	75	0.0	45.000004	1
AACGTAC	30	2.1649685E-6	45.000004	11
CTATGCG	35	1.2115379E-7	45.000004	1
TTGGCTA	30	2.1649685E-6	45.000004	22
CGGAACT	20	7.03246E-4	45.000004	13
GCCCATA	20	7.03246E-4	45.000004	27
TCGTTAA	20	7.03246E-4	45.000004	44
CGAACGT	20	7.03246E-4	45.000004	38
CTCGTTA	20	7.03246E-4	45.000004	43
TCACCGG	20	7.03246E-4	45.000004	2
GTCGATA	35	1.2115379E-7	45.000004	29
CTCAATC	25	3.8901206E-5	45.000004	27
GGTCGCT	20	7.03246E-4	45.000004	10
CGAGTAT	20	7.03246E-4	45.000004	42
CACGGGT	50	2.1827873E-11	45.000004	4
ACGCACT	25	3.8901206E-5	45.000004	15
CTATAGT	20	7.03246E-4	45.000004	40
GACGTAC	20	7.03246E-4	45.000004	9
CGCATGG	45	3.8380676E-10	45.000004	2
>>END_MODULE