FastQCFastQC Report
Thu 26 May 2016
SRR1547307_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547307_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2745685
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT629502.292688345531261No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCCG502.1827873E-1145.01
CGTTTTT275250.043.324251
AATAGCG900.040.01
TATAGCG1450.038.7931021
CGTTAGG4250.038.6470572
TACGGGA8000.038.254
TAATGCG1650.038.181821
GTTTTTT320850.038.0294572
AGTACGG2650.037.358492
AGGGCGA23600.037.2775426
TAAGGGA29550.037.157364
GACCGAT18600.037.1370939
ACGTTAG1700.037.0588261
GGGCGAT49450.037.037417
TTACGGG8150.036.9938623
ACGGGAT9800.036.9642875
TACGGGT2950.036.610174
TTATACG800.036.56251
AAGGGAT32350.036.445135
GGCGATA9450.036.190488
Produced by FastQC (version 0.11.5)