##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547307_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2745685
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.235682170387353	31.0	31.0	33.0	30.0	34.0
2	31.622937809690477	31.0	31.0	34.0	30.0	34.0
3	31.594857385315503	31.0	31.0	34.0	30.0	34.0
4	35.4515831204235	37.0	35.0	37.0	33.0	37.0
5	35.38339649304272	37.0	35.0	37.0	33.0	37.0
6	35.42202911113256	37.0	35.0	37.0	33.0	37.0
7	35.87178609345209	37.0	35.0	37.0	35.0	37.0
8	35.87509018696609	37.0	35.0	37.0	35.0	37.0
9	37.59516732618636	39.0	37.0	39.0	35.0	39.0
10	36.836362510630316	39.0	37.0	39.0	32.0	39.0
11	36.6081954047897	39.0	35.0	39.0	32.0	39.0
12	36.27273012017038	38.0	35.0	39.0	32.0	39.0
13	36.126102957913965	38.0	35.0	39.0	31.0	39.0
14	37.18434926074914	39.0	36.0	41.0	31.0	41.0
15	37.336795007438944	39.0	36.0	41.0	32.0	41.0
16	37.41716402282126	39.0	36.0	41.0	32.0	41.0
17	37.34487495834373	39.0	35.0	41.0	32.0	41.0
18	37.1286779073346	39.0	36.0	40.0	32.0	41.0
19	36.95684792683793	38.0	35.0	40.0	31.0	41.0
20	36.69677548589878	38.0	35.0	40.0	31.0	41.0
21	36.575366802819694	38.0	35.0	40.0	31.0	41.0
22	36.48515980529449	38.0	35.0	40.0	31.0	41.0
23	36.37815044333199	38.0	35.0	40.0	31.0	41.0
24	36.23612431870371	38.0	35.0	40.0	30.0	41.0
25	36.102127155882776	38.0	35.0	40.0	30.0	41.0
26	35.870326348434	37.0	35.0	40.0	30.0	41.0
27	35.82130360911758	37.0	34.0	40.0	30.0	41.0
28	35.79476378390092	38.0	34.0	40.0	30.0	41.0
29	35.736605983570584	38.0	34.0	40.0	29.0	41.0
30	35.64514720370327	37.0	34.0	40.0	29.0	41.0
31	35.48408867004044	37.0	34.0	40.0	28.0	41.0
32	35.17667540158467	37.0	34.0	40.0	26.0	41.0
33	34.84712048177413	37.0	34.0	40.0	24.0	41.0
34	34.46152854387885	37.0	33.0	40.0	23.0	41.0
35	34.200874463021066	37.0	33.0	40.0	21.0	41.0
36	33.906011432484064	37.0	33.0	40.0	20.0	41.0
37	33.829821337844656	37.0	33.0	40.0	20.0	41.0
38	33.73311323039606	37.0	33.0	40.0	19.0	41.0
39	33.57465441228692	37.0	33.0	40.0	18.0	41.0
40	33.452962011301366	36.0	33.0	40.0	18.0	41.0
41	33.39864623946301	36.0	33.0	40.0	18.0	41.0
42	33.29184629700785	36.0	32.0	40.0	18.0	41.0
43	33.16516825491635	36.0	32.0	40.0	18.0	41.0
44	33.0532941688504	36.0	32.0	40.0	17.0	41.0
45	32.90726685690456	35.0	31.0	40.0	17.0	41.0
46	32.8160673930185	35.0	31.0	40.0	17.0	41.0
47	32.77006065881556	35.0	31.0	40.0	17.0	41.0
48	32.61645308911984	35.0	31.0	40.0	17.0	41.0
49	32.47788657475275	35.0	31.0	39.0	15.0	41.0
50	32.34368363450287	35.0	31.0	39.0	15.0	41.0
51	31.906995522064623	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	4.0
12	10.0
13	19.0
14	48.0
15	153.0
16	368.0
17	932.0
18	2131.0
19	3939.0
20	6681.0
21	10590.0
22	15853.0
23	23260.0
24	33985.0
25	49423.0
26	67492.0
27	76323.0
28	74949.0
29	77025.0
30	83689.0
31	95992.0
32	114493.0
33	141778.0
34	204480.0
35	261709.0
36	250102.0
37	273686.0
38	362800.0
39	513596.0
40	171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.25405864110413	21.850576449956932	26.75241333219215	20.142951576746785
2	36.68738402256631	23.68713089811832	26.975126425646057	12.650358653669302
3	26.398111946563425	24.277184017831615	36.37696968151845	12.947734354086505
4	23.405707501042546	26.76840205631746	35.94381729877972	13.882073143860275
5	21.1909232122403	30.781608232553992	34.56193991663283	13.465528638572888
6	20.863463944334473	38.43933298976394	31.331234282155457	9.365968783746133
7	82.89953144661533	2.968075361886014	12.664963388007	1.4674298034916604
8	83.18201104642375	2.4905988851598053	12.688819001451368	1.6385710669650742
9	78.1411196113174	4.841742588825739	14.384862065386233	2.632275734470633
10	36.158335715859614	29.502510302529238	22.790524040448922	11.548629941162224
11	29.96039239752557	24.681928189140415	29.20910446755546	16.148574945778556
12	26.703026749244724	22.226584622780837	33.067995782473226	18.002392845501213
13	22.105194150093695	26.432820953605386	34.33179698326647	17.130187913034455
14	19.230465257303734	28.268938352360156	34.776021284306104	17.72457510603001
15	18.29976854591841	25.482675543625728	39.07010454586014	17.14745136459572
16	21.524027701648222	24.51344564289057	36.52640415779669	17.436122497664517
17	21.426711367108755	25.183478804014296	32.05415042147952	21.335659407397426
18	21.747724156267015	25.803360545729028	34.11414637877251	18.334768919231447
19	23.809359048834807	27.340244784088487	30.49235436694304	18.358041800133666
20	24.40622285513451	27.0534311109978	31.12898966924465	17.411356364623035
21	23.570402285768395	26.962561255205898	32.324611162606054	17.142425296419656
22	22.981988101329907	25.143343100173542	31.73965695263659	20.13501184585996
23	20.363697947870932	27.439200053902763	32.32031351010768	19.87678848811863
24	20.432023338438313	26.634847041812883	34.97094531965612	17.96218430009269
25	20.952439919364384	27.36992772295438	32.77247754203414	18.905154815647094
26	20.13665078113476	28.950079852568667	31.284870624270443	19.628398742026125
27	19.449572693153076	28.48454939295658	32.78092716389535	19.28495074999499
28	19.208722049324667	28.029253173616052	33.57377120827771	19.188253568781562
29	19.513855376709273	26.933606732017694	33.66078046097786	19.891757430295172
30	20.56991242622515	28.077474291479177	32.76217046019482	18.59044282210086
31	23.252776629511395	27.299089298298966	30.746935646295913	18.70119842589372
32	23.90485434418005	27.37080182176761	30.025913387733844	18.698430446318497
33	23.0243818937715	27.621923126651453	29.905105647588854	19.44858933198819
34	19.934624692927265	28.46218703165148	30.860349967312345	20.742838308108904
35	20.58477210605004	28.97670344558826	30.71339210433826	19.72513234402344
36	23.575391933160578	28.978925113405218	28.07110065429938	19.374582299134822
37	20.294862666329166	31.21195621493361	29.22658644382003	19.26659467491719
38	20.919005639758385	30.879361616500073	28.641122342876184	19.560510400865358
39	20.40081072664927	30.855360319920166	28.261508512447715	20.48232044098285
40	22.534995820715046	28.968399506862585	28.307507962493876	20.18909670992849
41	19.749534269226075	28.486989585476845	29.15680422189727	22.60667192339981
42	20.58954322873891	29.434330595097396	28.40194705510647	21.57417912105722
43	20.37116420856726	28.976594183236603	29.2130014914311	21.43924011676503
44	20.599850310578237	29.75009879137629	28.85502160663004	20.79502929141544
45	19.612519280252467	30.893929930053886	27.88502686943331	21.608523920260335
46	20.80566416030972	30.48102750315495	28.223667317991683	20.48964101854364
47	20.428417680833743	29.559836616363494	29.098348863762595	20.913396839040168
48	20.892891937713177	29.019716391355892	29.798392750807174	20.288998920123756
49	21.13315984899943	28.50341535900877	29.510559295767724	20.852865496224073
50	20.11308653396147	30.06830718017544	29.38661936820866	20.43198691765443
51	19.277994380273046	30.24305410125342	28.986901265075925	21.492050253397604
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2772.0
1	3539.5
2	4307.0
3	45154.5
4	86002.0
5	56319.0
6	26636.0
7	27889.5
8	29143.0
9	32464.5
10	35786.0
11	38666.5
12	41547.0
13	41546.0
14	41545.0
15	38791.5
16	36038.0
17	33239.0
18	30440.0
19	27613.0
20	24786.0
21	23787.5
22	22789.0
23	22999.5
24	23210.0
25	25298.5
26	29228.5
27	31070.0
28	35095.5
29	39121.0
30	43306.0
31	47491.0
32	52635.5
33	57780.0
34	69261.5
35	80743.0
36	82214.0
37	83685.0
38	94150.0
39	104615.0
40	117209.0
41	129803.0
42	140263.0
43	150723.0
44	157686.5
45	164650.0
46	171058.0
47	177466.0
48	180259.5
49	183053.0
50	177217.0
51	171381.0
52	163687.0
53	155993.0
54	147698.5
55	139404.0
56	130026.0
57	120648.0
58	117721.0
59	114794.0
60	103413.5
61	92033.0
62	84343.0
63	76653.0
64	69557.0
65	62461.0
66	52731.5
67	43002.0
68	36675.0
69	30348.0
70	25927.5
71	21507.0
72	18085.0
73	14663.0
74	11941.0
75	7455.5
76	5692.0
77	4181.0
78	2670.0
79	2096.0
80	1522.0
81	1089.5
82	657.0
83	436.5
84	216.0
85	165.0
86	114.0
87	90.0
88	66.0
89	48.5
90	31.0
91	22.5
92	14.0
93	9.5
94	5.0
95	4.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2745685.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.511898917891546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.44080288084405	21.06126535933085
2	6.613160155568444	3.5065486714451715
3	2.772872794548178	2.2054236972369825
4	1.6353896507066084	1.734291404435983
5	1.136654084874172	1.5067429101396284
6	0.813133334863822	1.2934625268406625
7	0.6297484700341536	1.1687079446869635
8	0.48156614779511464	1.0213786426098002
9	0.3996491245188775	0.95359114726815
>10	4.436158627172817	28.106040327749415
>50	1.3890250799892117	25.793129358439913
>100	0.2483561666999464	8.427639568186885
>500	0.002229428726119936	0.4072799619204241
>1k	0.001114714363059968	0.4890314862496666
>5k	0.0	0.0
>10k+	1.39339295382496E-4	2.325466993459474
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62950	2.292688345531261	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.284156776906309E-5	0.0	0.0	0.05208172095488011	0.0
2	7.284156776906309E-5	0.0	0.0	0.13708783054137674	0.0
3	7.284156776906309E-5	0.0	0.0	0.21615735235469474	0.0
4	7.284156776906309E-5	0.0	0.0	0.3248369714661369	0.0
5	7.284156776906309E-5	0.0	0.0	0.5801830872805875	0.0
6	7.284156776906309E-5	0.0	0.0	1.0642517258898962	0.0
7	7.284156776906309E-5	0.0	0.0	1.3838076836927762	0.0
8	7.284156776906309E-5	0.0	0.0	1.8788753990352134	0.0
9	7.284156776906309E-5	0.0	0.0	2.1717349222507316	0.0
10	7.284156776906309E-5	0.0	0.0	2.5394755771328468	0.0
11	7.284156776906309E-5	0.0	0.0	2.8546610408695825	0.0
12	7.284156776906309E-5	0.0	0.0	3.079705064492103	0.0
13	7.284156776906309E-5	0.0	0.0	3.222073908696737	0.0
14	7.284156776906309E-5	0.0	0.0	3.310612834320033	0.0
15	7.284156776906309E-5	0.0	0.0	3.3889539404556603	0.0
16	7.284156776906309E-5	0.0	0.0	3.513695125260181	0.0
17	7.284156776906309E-5	0.0	0.0	3.6447371056767253	0.0
18	7.284156776906309E-5	0.0	0.0	3.8126004986005313	0.0
19	7.284156776906309E-5	0.0	0.0	3.9175287769718667	0.0
20	1.0926235165359464E-4	0.0	0.0	4.025042930999004	0.0
21	1.0926235165359464E-4	0.0	0.0	4.15513797103455	0.0
22	1.0926235165359464E-4	0.0	0.0	4.301112472843753	0.0
23	1.0926235165359464E-4	0.0	0.0	4.468757341064252	0.0
24	1.0926235165359464E-4	0.0	0.0	4.603150033598173	0.0
25	1.0926235165359464E-4	0.0	0.0	4.725196080395238	0.0
26	1.0926235165359464E-4	0.0	0.0	4.841123435499703	0.0
27	1.0926235165359464E-4	0.0	0.0	4.97154626259021	0.0
28	1.0926235165359464E-4	0.0	0.0	5.0988369022666475	0.0
29	1.0926235165359464E-4	0.0	0.0	5.2440829883981595	0.0
30	1.0926235165359464E-4	0.0	0.0	5.426623957227432	0.0
31	1.0926235165359464E-4	0.0	0.0	5.580392506787923	0.0
32	1.0926235165359464E-4	0.0	0.0	5.729572037578965	0.0
33	1.0926235165359464E-4	0.0	0.0	5.87998987502208	0.0
34	1.8210391942265775E-4	0.0	0.0	6.034851048099108	0.0
35	1.8210391942265775E-4	0.0	0.0	6.205445999814254	0.0
36	1.8210391942265775E-4	0.0	0.0	6.358959603887555	0.0
37	1.8210391942265775E-4	0.0	0.0	6.52802488267955	0.0
38	1.8210391942265775E-4	0.0	0.0	6.719488943560532	0.0
39	1.8210391942265775E-4	0.0	0.0	7.000074662606964	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCCG	50	2.1827873E-11	45.0	1
CGTTTTT	27525	0.0	43.32425	1
AATAGCG	90	0.0	40.0	1
TATAGCG	145	0.0	38.793102	1
CGTTAGG	425	0.0	38.647057	2
TACGGGA	800	0.0	38.25	4
TAATGCG	165	0.0	38.18182	1
GTTTTTT	32085	0.0	38.029457	2
AGTACGG	265	0.0	37.35849	2
AGGGCGA	2360	0.0	37.277542	6
TAAGGGA	2955	0.0	37.15736	4
GACCGAT	1860	0.0	37.137093	9
ACGTTAG	170	0.0	37.058826	1
GGGCGAT	4945	0.0	37.03741	7
TTACGGG	815	0.0	36.993862	3
ACGGGAT	980	0.0	36.964287	5
TACGGGT	295	0.0	36.61017	4
TTATACG	80	0.0	36.5625	1
AAGGGAT	3235	0.0	36.44513	5
GGCGATA	945	0.0	36.19048	8
>>END_MODULE