FastQCFastQC Report
Thu 26 May 2016
SRR1547306_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547306_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences970143
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91520.943366081082892No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC207.032741E-445.00000419
CCCCGTA207.032741E-445.00000435
TGTTACG207.032741E-445.0000041
CGTCGAC207.032741E-445.00000437
TAGCACG253.890355E-545.01
TCGTACT253.890355E-545.021
GACGCAC253.890355E-545.09
GCGTAAG453.8380676E-1045.01
ACGGGAT3100.042.096775
CGGGTAT750.042.0000046
CGTTTTT42150.041.5302471
TATCGCG603.6379788E-1241.2499961
TCGATAG556.002665E-1140.9090921
TACGGGA3300.040.2272724
TCGTTAG451.927765E-840.01
TTACGAG451.927765E-840.01
TAACGCC403.4574805E-739.37500412
AAATGCG403.4574805E-739.3750041
CGTTAGG1550.039.1935462
GACCGAT5450.038.807349