FastQCFastQC Report
Thu 26 May 2016
SRR1547305_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547305_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences836024
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111581.334650679884788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG302.1647884E-645.0000041
CGATCGA207.032183E-445.010
TAAGACG253.88989E-545.01
GCGATCG650.044.9999969
CGTTTTT57150.042.4803121
ATACGAG750.042.01
TACGGGA3450.041.739134
CGTTAGG1150.041.0869562
TATACGG556.002665E-1140.9090922
CGTCATA556.002665E-1140.90909238
TAGGGAT13500.039.8333325
ACTAATC3700.039.52702710
GTTAGCG403.4567165E-739.3751
TTACCGG403.4567165E-739.3752
GGGCGAT15850.039.179817
GGCGATT3650.038.8356178
CGGGATA1800.038.756
TATGGGA9700.038.7371144
AGGGATC9550.038.6387446
CGACCCG700.038.57142632
Produced by FastQC (version 0.11.5)