##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547305_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	836024
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.301161210682945	31.0	31.0	33.0	30.0	34.0
2	31.706820617589926	31.0	31.0	34.0	30.0	34.0
3	31.75344128876683	31.0	31.0	34.0	30.0	34.0
4	35.580520415681846	37.0	35.0	37.0	33.0	37.0
5	35.50178703003741	37.0	35.0	37.0	33.0	37.0
6	35.53701089920864	37.0	35.0	37.0	33.0	37.0
7	35.9015375156694	37.0	35.0	37.0	35.0	37.0
8	35.890799785652085	37.0	35.0	37.0	35.0	37.0
9	37.63835009521258	39.0	37.0	39.0	35.0	39.0
10	36.978754198444065	39.0	37.0	39.0	32.0	39.0
11	36.67581672296489	39.0	35.0	39.0	32.0	39.0
12	36.40765815335445	39.0	35.0	39.0	32.0	39.0
13	36.32679085767872	39.0	35.0	39.0	32.0	39.0
14	37.42752002334861	39.0	36.0	41.0	32.0	41.0
15	37.5260183918165	39.0	36.0	41.0	32.0	41.0
16	37.57692841353837	40.0	36.0	41.0	32.0	41.0
17	37.47917284671254	39.0	36.0	41.0	32.0	41.0
18	37.30408098332105	39.0	36.0	41.0	32.0	41.0
19	37.207708151919086	39.0	36.0	41.0	32.0	41.0
20	37.02597891926548	39.0	35.0	41.0	32.0	41.0
21	36.923901706171115	39.0	35.0	41.0	31.0	41.0
22	36.82902883170818	38.0	35.0	41.0	31.0	41.0
23	36.727182473230435	38.0	35.0	40.0	31.0	41.0
24	36.59970886003273	38.0	35.0	40.0	31.0	41.0
25	36.49216410055214	38.0	35.0	40.0	30.0	41.0
26	36.25553213783336	38.0	35.0	40.0	30.0	41.0
27	36.253519037730975	38.0	35.0	40.0	30.0	41.0
28	36.27516434934882	38.0	35.0	40.0	30.0	41.0
29	36.25128465211525	38.0	35.0	40.0	30.0	41.0
30	36.208061012602506	38.0	35.0	40.0	30.0	41.0
31	36.1282570835287	38.0	35.0	40.0	30.0	41.0
32	35.918137517583226	38.0	35.0	40.0	29.0	41.0
33	35.67839440016076	38.0	35.0	40.0	27.0	41.0
34	35.383294020267364	38.0	34.0	40.0	26.0	41.0
35	35.19630776149967	38.0	34.0	40.0	25.0	41.0
36	34.957714132608636	38.0	34.0	40.0	24.0	41.0
37	34.92705472570165	38.0	34.0	40.0	24.0	41.0
38	34.83357056735213	38.0	34.0	40.0	24.0	41.0
39	34.68869434370305	38.0	34.0	40.0	23.0	41.0
40	34.56433786589859	38.0	33.0	40.0	23.0	41.0
41	34.521736218099	38.0	33.0	40.0	23.0	41.0
42	34.42562414476139	37.0	33.0	40.0	23.0	41.0
43	34.310052103767354	37.0	33.0	40.0	23.0	41.0
44	34.20190927533181	37.0	33.0	40.0	22.0	41.0
45	34.062165679454175	37.0	33.0	40.0	22.0	41.0
46	33.98128642239936	37.0	33.0	40.0	22.0	41.0
47	33.932295005884995	37.0	33.0	40.0	22.0	41.0
48	33.79354898902424	36.0	33.0	40.0	22.0	41.0
49	33.66190803134838	36.0	33.0	40.0	21.0	41.0
50	33.53274307914605	36.0	32.0	40.0	20.0	41.0
51	33.06561534118638	35.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	4.0
13	5.0
14	11.0
15	30.0
16	90.0
17	212.0
18	485.0
19	801.0
20	1513.0
21	2285.0
22	3551.0
23	5144.0
24	7534.0
25	11002.0
26	15076.0
27	17954.0
28	18872.0
29	20421.0
30	22877.0
31	27079.0
32	32745.0
33	41581.0
34	60416.0
35	74314.0
36	73186.0
37	89485.0
38	124735.0
39	184554.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.41139010363339	22.660593475785383	27.493349473220864	17.434666947360363
2	34.191721768753055	24.554319014765127	28.35026267188502	12.903696544596807
3	28.138665875620795	24.956221352497057	33.488990746677125	13.416122025205018
4	24.8839746227381	27.753987923791662	33.00228223113212	14.359755222338114
5	22.477345147986185	32.018219572643844	31.892505478311627	13.611929801058341
6	21.924729433604778	40.353985053060676	28.29799144522167	9.423294068112877
7	87.4383988976393	2.5716965063203925	8.800584672210366	1.189319923829938
8	87.55825191621294	2.311656124704554	8.839817995655627	1.2902739634268874
9	82.65922987856807	4.975216022506531	10.089782111518328	2.27577198740706
10	38.178927877668585	35.96380008229429	15.626465268939649	10.23080677109748
11	28.48781853152541	24.97547917284671	29.417337301321496	17.119364994306384
12	27.43031300536827	22.992162904414226	31.270992220319034	18.30653186989847
13	23.957087356343838	24.823928499660298	32.34237294623121	18.876611197764657
14	21.243528893907353	26.656172550070334	32.342971015186166	19.757327540836148
15	20.381113460857584	25.393290144780533	35.236069777901115	18.989526616460772
16	23.903141536606604	24.95777633177995	33.116393787738154	18.022688343875295
17	23.950508597839296	24.693788695061386	30.732730160856626	20.62297254624269
18	23.740347167066975	24.882778484828187	32.15864616326804	19.2182281848368
19	25.14389539056295	26.154751538233352	29.515779451307615	19.18557361989608
20	26.282857908385402	26.130350324871053	29.796154177392037	17.7906375893515
21	25.89722304622834	25.56673014171842	30.652828148474203	17.883218663579036
22	24.92727481507708	23.96223074935648	30.678664727328403	20.43182970823804
23	23.14395280518263	26.210013109671493	30.452355434772205	20.193678650373673
24	22.279982392849966	25.71433355980211	32.828244165239276	19.17743988210865
25	23.049218682717243	26.96788608939456	30.11468570280279	19.868209525085405
26	22.14445996765643	28.015583284690393	29.50656918940126	20.333387558251918
27	21.171401777939387	27.474570108035174	30.837870683138284	20.51615743088715
28	20.597016353597507	27.481746935494677	31.819660679597717	20.101576031310106
29	21.43060486301829	25.985976479144142	31.662727385816673	20.9206912720209
30	21.657033769365473	26.57674899285188	32.111877170990304	19.654340066792344
31	23.840583523917974	26.937863027855656	29.770437212328833	19.451116235897533
32	24.225620317120082	26.547802455431903	29.338033357894034	19.888543869553985
33	23.632694755174494	27.45531228768552	29.002277446580482	19.909715510559504
34	21.53765920595581	27.737481220634812	29.93335119565945	20.79150837774992
35	22.24995933131106	28.3734677473374	29.086246327856617	20.290326593494925
36	23.155555342908816	29.163875678210193	27.424332315818685	20.256236663062303
37	22.57602652555429	29.075241859085388	27.78580519219544	20.562926423164885
38	22.49050266499526	29.03899888041492	27.753629082418684	20.716869372171136
39	22.44815938298422	28.80910117412897	27.608776781527805	21.133962661359003
40	23.270384582260796	27.704707041903102	28.208400715768928	20.816507660067174
41	21.30142196874731	27.79704888854865	28.746543161440343	22.154985981263696
42	22.207974890672997	27.874917466484217	27.695377166205752	22.221730476637035
43	21.700812416868416	27.42971493641331	28.655397452704705	22.214075194013567
44	21.58909313608222	27.80195305397931	28.631235466924394	21.97771834301408
45	21.279413031204847	28.624058639464895	28.00146885735338	22.09505947197688
46	21.85523382103863	28.977876233218186	28.037113767068888	21.129776178674298
47	21.577849439729	28.10062869008545	28.99653598461288	21.324985885572666
48	21.704998899553125	27.465240232337827	29.94052802311895	20.889232844990097
49	22.175200711941283	27.474211266662202	28.803718553534345	21.546869467862166
50	20.87428112111614	28.479325952365002	29.597236442972928	21.04915648354593
51	21.123795489124713	28.444518339186438	28.669990335205686	21.761695836483163
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	548.0
1	863.0
2	1178.0
3	8959.5
4	16741.0
5	11325.0
6	5909.0
7	5840.5
8	5772.0
9	6131.0
10	6490.0
11	6745.5
12	7001.0
13	6914.0
14	6827.0
15	6473.5
16	6120.0
17	5763.5
18	5407.0
19	5265.0
20	5123.0
21	5036.0
22	4949.0
23	5428.0
24	5907.0
25	6539.0
26	8026.0
27	8881.0
28	10750.0
29	12619.0
30	14609.5
31	16600.0
32	18787.5
33	20975.0
34	23265.0
35	25555.0
36	28154.5
37	30754.0
38	33333.5
39	35913.0
40	38919.5
41	41926.0
42	45282.0
43	48638.0
44	49442.5
45	50247.0
46	51105.0
47	51963.0
48	53597.5
49	55232.0
50	55054.5
51	54877.0
52	52692.5
53	50508.0
54	47925.5
55	45343.0
56	44294.0
57	43245.0
58	40661.5
59	38078.0
60	35234.0
61	32390.0
62	29237.5
63	26085.0
64	22786.5
65	19488.0
66	16804.0
67	14120.0
68	12110.5
69	10101.0
70	8200.0
71	6299.0
72	5366.5
73	4434.0
74	3611.5
75	2354.5
76	1920.0
77	1446.5
78	973.0
79	752.5
80	532.0
81	363.0
82	194.0
83	152.5
84	111.0
85	84.0
86	57.0
87	37.0
88	17.0
89	15.0
90	13.0
91	8.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	836024.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.718718088935077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.84487113548035	22.471084955932348
2	6.629852059780046	4.205808169110177
3	3.519883402533498	3.349385680526449
4	2.5074236588258585	3.181290566552941
5	1.9976362071331046	3.1681229849152235
6	1.702979967660149	3.240980490318962
7	1.455505136998148	3.2316729982208763
8	1.2418309469223727	3.1511428575636833
9	1.1088095391723376	3.1653015468596455
>10	8.926312100225857	47.772989863332256
>50	0.051233565619235644	1.0233081524808083
>100	0.013282771880979545	0.6957674946038016
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	3.7950776802794176E-4	1.343144239582808
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11158	1.334650679884788	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.023563916825354296	0.0
2	0.0	0.0	0.0	0.05837153000392333	0.0
3	0.0	0.0	0.0	0.10215017750686584	0.0
4	0.0	0.0	0.0	0.14580921121881668	0.0
5	0.0	0.0	0.0	0.24161985780312528	0.0
6	0.0	0.0	0.0	0.46685262624039503	0.0
7	0.0	0.0	0.0	0.5968728170483144	0.0
8	0.0	0.0	0.0	0.8167229648909601	0.0
9	0.0	0.0	0.0	0.9491354315187124	0.0
10	0.0	0.0	0.0	1.127120752514282	0.0
11	0.0	0.0	0.0	1.2942212185296116	0.0
12	0.0	0.0	0.0	1.4323751471249628	0.0
13	0.0	0.0	0.0	1.537156828033645	0.0
14	0.0	0.0	0.0	1.6164607714611063	0.0
15	0.0	0.0	0.0	1.679856080686679	0.0
16	0.0	0.0	0.0	1.7567677482943074	0.0
17	0.0	0.0	0.0	1.8359520779307772	0.0
18	0.0	0.0	0.0	1.934394227916902	0.0
19	0.0	0.0	0.0	2.0011387232902407	0.0
20	0.0	0.0	0.0	2.079724983971752	0.0
21	0.0	0.0	0.0	2.1678803479325954	0.0
22	0.0	0.0	0.0	2.2567533946393885	0.0
23	0.0	0.0	0.0	2.3551955446255133	0.0
24	0.0	0.0	0.0	2.445982411988173	0.0
25	0.0	0.0	0.0	2.523252920968776	0.0
26	0.0	0.0	0.0	2.6069825746629283	0.0
27	0.0	0.0	0.0	2.6994440350994706	0.0
28	0.0	0.0	0.0	2.789752447298164	0.0
29	0.0	0.0	0.0	2.889988804149163	0.0
30	0.0	0.0	0.0	3.009602595140809	0.0
31	0.0	0.0	0.0	3.116896165660316	0.0
32	0.0	0.0	0.0	3.2295723567744465	0.0
33	0.0	0.0	0.0	3.334952106638087	0.0
34	0.0	0.0	0.0	3.4502598011540337	0.0
35	0.0	0.0	0.0	3.575973895486254	0.0
36	0.0	0.0	0.0	3.6957073002688916	0.0
37	0.0	0.0	0.0	3.816636842961446	0.0
38	0.0	0.0	0.0	3.961010688688363	0.0
39	0.0	0.0	0.0	4.1596891955254875	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	30	2.1647884E-6	45.000004	1
CGATCGA	20	7.032183E-4	45.0	10
TAAGACG	25	3.88989E-5	45.0	1
GCGATCG	65	0.0	44.999996	9
CGTTTTT	5715	0.0	42.480312	1
ATACGAG	75	0.0	42.0	1
TACGGGA	345	0.0	41.73913	4
CGTTAGG	115	0.0	41.086956	2
TATACGG	55	6.002665E-11	40.909092	2
CGTCATA	55	6.002665E-11	40.909092	38
TAGGGAT	1350	0.0	39.833332	5
ACTAATC	370	0.0	39.527027	10
GTTAGCG	40	3.4567165E-7	39.375	1
TTACCGG	40	3.4567165E-7	39.375	2
GGGCGAT	1585	0.0	39.17981	7
GGCGATT	365	0.0	38.835617	8
CGGGATA	180	0.0	38.75	6
TATGGGA	970	0.0	38.737114	4
AGGGATC	955	0.0	38.638744	6
CGACCCG	70	0.0	38.571426	32
>>END_MODULE