FastQCFastQC Report
Thu 26 May 2016
SRR1547304_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547304_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2835366
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT426711.5049556212496022No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTT207.035039E-445.019
CGTTTTT208050.042.912761
CGTTAGG4200.041.2500042
GCGATAC2200.039.886369
TAGTACG1250.039.61
CGGTAAT403.4606092E-739.37523
TACGGGA12800.039.199224
TGGGCGA17900.038.966486
GGGCGAT58250.038.9356237
AGGGCGA27000.038.9166686
ACGGGAT16650.038.648655
TAGGGCG8600.038.45935
GGCGATA11350.038.458158
CGAGGGA24250.038.2268034
TAGGGAT50200.038.097615
GACCGAT15900.037.9245269
TATGGGA43350.037.837374
AACGCGA301.1403441E-437.50000417
TTAGGGA39950.037.4530644
AGGGATC40750.037.4355856
Produced by FastQC (version 0.11.5)