##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547304_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2835366 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.315005893419052 31.0 31.0 33.0 30.0 34.0 2 31.716758259780217 31.0 31.0 34.0 30.0 34.0 3 31.74827447320734 31.0 31.0 34.0 30.0 34.0 4 35.557226121777575 37.0 35.0 37.0 33.0 37.0 5 35.4706902036633 37.0 35.0 37.0 33.0 37.0 6 35.50581582765682 37.0 35.0 37.0 33.0 37.0 7 35.94194788256613 37.0 35.0 37.0 35.0 37.0 8 35.94724173175526 37.0 35.0 37.0 35.0 37.0 9 37.68064651970857 39.0 37.0 39.0 35.0 39.0 10 36.98237582026447 39.0 37.0 39.0 32.0 39.0 11 36.66652629678144 39.0 35.0 39.0 32.0 39.0 12 36.494343940076874 39.0 35.0 39.0 32.0 39.0 13 36.445013095311154 39.0 35.0 39.0 32.0 39.0 14 37.561006586098586 40.0 36.0 41.0 32.0 41.0 15 37.66241712710105 40.0 36.0 41.0 32.0 41.0 16 37.696817976938426 40.0 36.0 41.0 32.0 41.0 17 37.599002033599895 40.0 36.0 41.0 32.0 41.0 18 37.42492397806844 39.0 36.0 41.0 32.0 41.0 19 37.318449187864985 39.0 36.0 41.0 32.0 41.0 20 37.13823929609087 39.0 35.0 41.0 32.0 41.0 21 37.02792866952626 39.0 35.0 41.0 31.0 41.0 22 36.93734071721252 39.0 35.0 41.0 31.0 41.0 23 36.81815046099869 38.0 35.0 40.0 31.0 41.0 24 36.719973717678776 38.0 35.0 40.0 31.0 41.0 25 36.62623696552755 38.0 35.0 40.0 31.0 41.0 26 36.42600214575473 38.0 35.0 40.0 30.0 41.0 27 36.408716546646886 38.0 35.0 40.0 30.0 41.0 28 36.42687787044071 38.0 35.0 40.0 30.0 41.0 29 36.406610292992156 38.0 35.0 40.0 30.0 41.0 30 36.355543517133235 38.0 35.0 40.0 30.0 41.0 31 36.259333715647294 38.0 35.0 40.0 30.0 41.0 32 36.041563593553704 38.0 35.0 40.0 29.0 41.0 33 35.801301137137145 38.0 35.0 40.0 28.0 41.0 34 35.51991488929472 38.0 35.0 40.0 26.0 41.0 35 35.32840592713604 38.0 34.0 40.0 25.0 41.0 36 35.099410093793885 38.0 34.0 40.0 24.0 41.0 37 35.067543308341854 38.0 34.0 40.0 24.0 41.0 38 34.97571636254367 38.0 34.0 40.0 24.0 41.0 39 34.83796589223402 38.0 34.0 40.0 23.0 41.0 40 34.75241150525188 38.0 34.0 40.0 23.0 41.0 41 34.71210312883769 38.0 34.0 40.0 23.0 41.0 42 34.62766217835722 38.0 34.0 40.0 23.0 41.0 43 34.513211698242834 38.0 33.0 40.0 23.0 41.0 44 34.42071288151159 38.0 33.0 40.0 23.0 41.0 45 34.2662421712047 38.0 33.0 40.0 22.0 41.0 46 34.197208402724726 37.0 33.0 40.0 22.0 41.0 47 34.1710650406332 37.0 33.0 40.0 22.0 41.0 48 34.0442218041692 37.0 33.0 40.0 22.0 41.0 49 33.90999715733348 37.0 33.0 40.0 21.0 41.0 50 33.802884706947886 37.0 33.0 40.0 20.0 41.0 51 33.35051030448979 36.0 32.0 40.0 20.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 3.0 11 2.0 12 6.0 13 8.0 14 26.0 15 93.0 16 250.0 17 660.0 18 1347.0 19 2591.0 20 4619.0 21 7237.0 22 11355.0 23 16904.0 24 24683.0 25 36810.0 26 51755.0 27 61388.0 28 64590.0 29 68144.0 30 75782.0 31 88802.0 32 107019.0 33 132811.0 34 189362.0 35 241418.0 36 236877.0 37 293078.0 38 427860.0 39 689652.0 40 233.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.32315193170829 23.110455581395843 28.01871081193751 17.547681674958366 2 34.14374017322631 24.30455186385109 28.712942173955675 12.838765788966928 3 27.78621878092634 24.896538930071106 34.53652897015764 12.780713318844905 4 23.856955327813058 27.53196589082327 34.15040597933389 14.460672802029793 5 22.181369177735785 31.94705727585081 32.3674262864124 13.504147260001002 6 21.4926750197329 39.783788054170074 29.28376795094531 9.43976897515171 7 87.48775290385792 2.2347732179901993 9.249176296816708 1.0282975813351787 8 87.95686341728016 1.9103001164576285 9.057701898097106 1.0751345681650977 9 82.93119830032525 5.068234577123377 10.201716462707108 1.798850659844267 10 35.44900376177185 39.18859152575011 15.642601343177564 9.719803369300472 11 24.0276563942715 25.723557382009943 33.09773764656838 17.15104857715018 12 24.19137423528391 23.722686947646267 33.52646536637598 18.559473450693844 13 22.1291713309675 25.7276132957791 34.283510488593 17.859704884660392 14 19.017121599116305 27.723898784142857 34.34149242108426 18.917487195656573 15 18.658966778892037 26.45496207544282 37.000761101035984 17.88531004462916 16 22.359935190024853 25.708744479548674 34.83148207321383 17.09983825721265 17 21.860810914710836 25.226655042065115 32.87339271191092 20.039141331313136 18 21.80984747648099 25.150544938466496 34.4422201578209 18.597387427231617 19 23.53586803255735 26.237882516754453 31.741651695054536 18.484597755633665 20 24.663235716306115 26.801971950005747 31.160774305680466 17.374018028007672 21 24.13229896951575 25.388926861646787 33.33922322550246 17.139550943335006 22 23.64146286581697 23.620936415263497 32.65871143266866 20.078889286250874 23 20.581434636657136 27.243149561643893 32.92358023620231 19.25183556549666 24 20.064252727866528 26.52207863111852 34.66282659804766 18.750842042967292 25 21.252141698814192 27.574359006914804 32.5652843407165 18.608214953554498 26 20.479825179535904 28.916196357013526 30.805476259502303 19.798502203948274 27 19.55902694749108 28.07464715313649 33.132724311429286 19.233601587943145 28 18.811892362396954 28.301460904870833 34.1054735085347 18.781173224197513 29 20.212487558925375 26.361640789936818 33.87788384286191 19.5479878082759 30 20.70797914625484 26.664882064608236 33.37431569680952 19.25282309232741 31 22.351823362486538 26.87483732258904 31.97520179052722 18.798137524397205 32 22.79370635043236 27.554149975699787 31.181089143341634 18.471054530526217 33 21.855661667664776 27.468693636024415 31.33461429670808 19.341030399602733 34 20.187728850525826 28.3708699335465 32.2413402714147 19.200060944512984 35 20.45732367532093 28.49501616369809 31.6536912694869 19.39396889149408 36 22.122470256044547 29.256152468499657 29.47347890889571 19.147898366560085 37 21.004836765341757 29.17820838650107 30.555385089614536 19.261569758542635 38 20.83692193529865 29.833820395673786 30.22548059051283 19.10377707851473 39 20.578683669057188 29.137896130517188 29.693979542676324 20.5894406577493 40 21.992963165954592 28.178055319842304 29.87476749033458 19.954214023868523 41 19.93139510031509 28.447332725298956 30.39120875400213 21.230063420383825 42 21.019261710833803 28.78912281518506 29.615541697262366 20.576073776718772 43 21.055694397125453 28.386811438100057 29.92230985347218 20.635184311302314 44 20.824930538068102 29.123153765686688 29.726744272168037 20.325171424077173 45 20.29353529667775 29.014032050888666 29.831668997935363 20.86076365449822 46 21.255174816937213 29.1530617211323 29.483847940618602 20.107915521311888 47 20.338503036292316 28.959047967705054 30.404222946878818 20.298226049123816 48 20.637300440225353 28.730012280601517 30.713742070688582 19.918945208484548 49 20.800207098483938 28.245454026041084 30.64158207441297 20.312756801062015 50 20.041327997866944 29.12805613102506 30.921757543823265 19.908858327284733 51 19.575038989675406 29.28916407969906 30.1348750037914 21.00092192683414 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1834.0 1 2783.0 2 3732.0 3 33766.0 4 63800.0 5 42900.5 6 22001.0 7 21765.5 8 21530.0 9 22544.0 10 23558.0 11 24073.0 12 24588.0 13 24489.0 14 24390.0 15 23441.0 16 22492.0 17 21636.5 18 20781.0 19 20065.5 20 19350.0 21 19232.0 22 19114.0 23 19725.0 24 20336.0 25 23456.5 26 31834.0 27 37091.0 28 43452.0 29 49813.0 30 56824.0 31 63835.0 32 75305.0 33 86775.0 34 96305.0 35 105835.0 36 114321.5 37 122808.0 38 132250.5 39 141693.0 40 154835.0 41 167977.0 42 173827.0 43 179677.0 44 189797.5 45 199918.0 46 204316.5 47 208715.0 48 207272.0 49 205829.0 50 199751.5 51 193674.0 52 181409.5 53 169145.0 54 154887.5 55 140630.0 56 128105.0 57 115580.0 58 105320.0 59 95060.0 60 83841.5 61 72623.0 62 64385.0 63 56147.0 64 47792.5 65 39438.0 66 32393.5 67 25349.0 68 20598.0 69 15847.0 70 12944.5 71 10042.0 72 8571.5 73 7101.0 74 6003.5 75 4184.0 76 3462.0 77 2307.0 78 1152.0 79 888.5 80 625.0 81 458.5 82 292.0 83 208.5 84 125.0 85 94.5 86 64.0 87 43.0 88 22.0 89 16.5 90 11.0 91 11.0 92 11.0 93 10.0 94 9.0 95 5.0 96 1.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2835366.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.718374741863325 #Duplication Level Percentage of deduplicated Percentage of total 1 77.80771734139145 17.67664880370709 2 6.436592583924064 2.9245784476457053 3 2.4189024741654555 1.6486059861633355 4 1.5288128861869361 1.3892857623433785 5 1.0425104473703803 1.184207150783394 6 0.7708592754352062 1.050760193554695 7 0.6396860150915227 1.0172838625584915 8 0.5209289155623861 0.9467726654095012 9 0.43979262626652044 0.899223632300795 >10 6.382342533823174 35.52599347485392 >50 1.8077658688866423 28.081937188103385 >100 0.20188484541724602 5.774581124203525 >500 0.0015744189135101965 0.23953989576463966 >1k 4.723256740530589E-4 0.11431529145345502 >5k 0.0 0.0 >10k+ 1.5744189135101966E-4 1.526266521154687 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 42671 1.5049556212496022 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.053763076795023E-5 0.0 0.0 0.022148816061136375 0.0 2 7.053763076795023E-5 0.0 0.0 0.059921717337373726 0.0 3 7.053763076795023E-5 0.0 0.0 0.09808257558283481 0.0 4 7.053763076795023E-5 0.0 0.0 0.1557823575510181 0.0 5 7.053763076795023E-5 0.0 0.0 0.27703654484112455 0.0 6 7.053763076795023E-5 0.0 0.0 0.5435982515132085 0.0 7 7.053763076795023E-5 0.0 0.0 0.7044240496641351 0.0 8 7.053763076795023E-5 0.0 0.0 0.9898193037512617 0.0 9 7.053763076795023E-5 0.0 0.0 1.1469418762868708 0.0 10 7.053763076795023E-5 0.0 0.0 1.3390158448679994 0.0 11 1.0580644615192536E-4 0.0 0.0 1.505308309403442 0.0 12 1.0580644615192536E-4 0.0 0.0 1.6378485176164206 0.0 13 1.0580644615192536E-4 0.0 0.0 1.725138835691759 0.0 14 1.0580644615192536E-4 0.0 0.0 1.7865771120906437 0.0 15 1.0580644615192536E-4 0.0 0.0 1.83694098045896 0.0 16 1.0580644615192536E-4 0.0 0.0 1.9063500091346233 0.0 17 1.0580644615192536E-4 0.0 0.0 1.988737961871589 0.0 18 1.0580644615192536E-4 0.0 0.0 2.093662687638915 0.0 19 1.0580644615192536E-4 0.0 0.0 2.170372361099061 0.0 20 1.0580644615192536E-4 0.0 0.0 2.2474699915284306 0.0 21 1.0580644615192536E-4 0.0 0.0 2.3428015995113154 0.0 22 1.0580644615192536E-4 0.0 0.0 2.4501951423555197 0.0 23 1.0580644615192536E-4 0.0 0.0 2.5636196526303836 0.0 24 1.0580644615192536E-4 0.0 0.0 2.6614553465055306 0.0 25 1.0580644615192536E-4 0.0 0.0 2.7526252342731063 0.0 26 1.0580644615192536E-4 0.0 0.0 2.8358596385792874 0.0 27 1.0580644615192536E-4 0.0 0.0 2.9319318916852355 0.0 28 1.0580644615192536E-4 0.0 0.0 3.032060058560341 0.0 29 1.0580644615192536E-4 0.0 0.0 3.144214891481382 0.0 30 1.0580644615192536E-4 0.0 0.0 3.2902983248018067 0.0 31 1.4107526153590047E-4 0.0 0.0 3.414903049553391 0.0 32 1.4107526153590047E-4 0.0 0.0 3.5375327206434726 0.0 33 1.4107526153590047E-4 0.0 0.0 3.661396800271993 0.0 34 1.4107526153590047E-4 0.0 0.0 3.793407976254212 0.0 35 1.4107526153590047E-4 0.0 0.0 3.9359997968516236 0.0 36 1.4107526153590047E-4 0.0 0.0 4.074676778941414 0.0 37 1.4107526153590047E-4 0.0 0.0 4.205453546385193 0.0 38 1.4107526153590047E-4 0.0 0.0 4.361412248013131 0.0 39 1.763440769198756E-4 0.0 0.0 4.5844875053167735 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCGTT 20 7.035039E-4 45.0 19 CGTTTTT 20805 0.0 42.91276 1 CGTTAGG 420 0.0 41.250004 2 GCGATAC 220 0.0 39.88636 9 TAGTACG 125 0.0 39.6 1 CGGTAAT 40 3.4606092E-7 39.375 23 TACGGGA 1280 0.0 39.19922 4 TGGGCGA 1790 0.0 38.96648 6 GGGCGAT 5825 0.0 38.935623 7 AGGGCGA 2700 0.0 38.916668 6 ACGGGAT 1665 0.0 38.64865 5 TAGGGCG 860 0.0 38.4593 5 GGCGATA 1135 0.0 38.45815 8 CGAGGGA 2425 0.0 38.226803 4 TAGGGAT 5020 0.0 38.09761 5 GACCGAT 1590 0.0 37.924526 9 TATGGGA 4335 0.0 37.83737 4 AACGCGA 30 1.1403441E-4 37.500004 17 TTAGGGA 3995 0.0 37.453064 4 AGGGATC 4075 0.0 37.435585 6 >>END_MODULE