FastQCFastQC Report
Thu 26 May 2016
SRR1547303_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547303_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2641284
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211200.799611098238584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAC5800.040.3448265
CGTTTTT106850.039.883011
TGCGATA403.4605E-739.3751
GGGCGAT47150.038.7486767
CGTAAGC356.251372E-638.57142637
CGTCGAA356.251372E-638.57142631
AGGGCGA26300.038.0703436
TTAGGGA29150.037.590054
TAAGGGA34000.037.5882384
TAACGCC900.037.512
CGAATAT2100.037.514
TAGTACG900.037.51
AGGGATT39050.037.336756
CGTTAGG3150.037.1428572
TATCACG850.037.0588231
CATACGA9400.036.86170218
TACGGGA9150.036.6393434
TAACGGG7400.036.4864853
AGTAGGG38400.036.2109383
GTTTACG1000.036.0000041
Produced by FastQC (version 0.11.5)