##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547303_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2641284
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.375517740614036	31.0	31.0	33.0	30.0	34.0
2	31.789820784133777	31.0	31.0	34.0	30.0	34.0
3	31.88486205951348	31.0	31.0	34.0	30.0	34.0
4	35.668235979167704	37.0	35.0	37.0	33.0	37.0
5	35.57763951169204	37.0	35.0	37.0	33.0	37.0
6	35.61194820397958	37.0	35.0	37.0	33.0	37.0
7	35.955401615274994	37.0	35.0	37.0	35.0	37.0
8	35.94939695996341	37.0	35.0	37.0	35.0	37.0
9	37.68771854900874	39.0	37.0	39.0	35.0	39.0
10	37.02220889537058	39.0	37.0	39.0	33.0	39.0
11	36.74547909274429	39.0	37.0	39.0	32.0	39.0
12	36.36195161141324	38.0	35.0	39.0	32.0	39.0
13	36.20510478994307	38.0	35.0	39.0	32.0	39.0
14	37.28573754280115	39.0	36.0	41.0	32.0	41.0
15	37.43578880574751	39.0	35.0	41.0	32.0	41.0
16	37.53999683487274	39.0	35.0	41.0	32.0	41.0
17	37.451597783502265	39.0	35.0	41.0	32.0	41.0
18	37.33543004084377	39.0	36.0	41.0	32.0	41.0
19	37.2690702703685	39.0	35.0	41.0	32.0	41.0
20	37.13704281705413	39.0	35.0	41.0	32.0	41.0
21	36.98961527802387	39.0	35.0	41.0	31.0	41.0
22	36.90117571605325	39.0	35.0	41.0	31.0	41.0
23	36.789948752197795	38.0	35.0	40.0	31.0	41.0
24	36.67162789007165	38.0	35.0	40.0	31.0	41.0
25	36.579802474856926	38.0	35.0	40.0	31.0	41.0
26	36.35554222870392	38.0	35.0	40.0	30.0	41.0
27	36.336586296664805	38.0	35.0	40.0	30.0	41.0
28	36.38313941249786	38.0	35.0	40.0	30.0	41.0
29	36.399654486227156	38.0	35.0	40.0	30.0	41.0
30	36.36769086550329	38.0	35.0	40.0	30.0	41.0
31	36.30107440169251	38.0	35.0	40.0	30.0	41.0
32	36.13981343922123	38.0	35.0	40.0	30.0	41.0
33	35.98143819445391	38.0	35.0	40.0	30.0	41.0
34	35.786984284915974	38.0	35.0	40.0	29.0	41.0
35	35.66157293195279	38.0	35.0	40.0	28.0	41.0
36	35.43896718414226	38.0	34.0	40.0	27.0	41.0
37	35.41042046216916	38.0	34.0	40.0	27.0	41.0
38	35.31619545645224	38.0	34.0	40.0	27.0	41.0
39	35.183577002700204	38.0	34.0	40.0	26.0	41.0
40	35.04823108760739	38.0	34.0	40.0	25.0	41.0
41	35.008877500488396	38.0	34.0	40.0	25.0	41.0
42	34.92239001939966	38.0	34.0	40.0	25.0	41.0
43	34.81904217797102	37.0	34.0	40.0	25.0	41.0
44	34.72180840833473	37.0	34.0	40.0	24.0	41.0
45	34.565604456014576	37.0	33.0	40.0	24.0	41.0
46	34.481094422258266	37.0	33.0	40.0	24.0	41.0
47	34.42827692894819	37.0	33.0	40.0	24.0	41.0
48	34.312861850524214	36.0	33.0	40.0	23.0	41.0
49	34.171737306552416	36.0	33.0	40.0	24.0	41.0
50	34.04883382476099	36.0	33.0	40.0	23.0	41.0
51	33.576527931112295	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	4.0
13	20.0
14	47.0
15	87.0
16	198.0
17	609.0
18	1169.0
19	2315.0
20	3943.0
21	6301.0
22	9744.0
23	13792.0
24	20131.0
25	28424.0
26	37725.0
27	45662.0
28	50998.0
29	58031.0
30	68224.0
31	82461.0
32	102577.0
33	132534.0
34	203630.0
35	265066.0
36	225029.0
37	280209.0
38	395971.0
39	606197.0
40	181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.42949868321619	23.388283880112855	28.43760837532049	14.744609061350463
2	32.10820949205008	25.372583940235128	28.982116273751707	13.53709029396309
3	28.856003368058868	25.897404444202138	31.52171443888654	13.724877748852451
4	25.610082066146617	28.074186645586007	31.250861323507813	15.064869964759565
5	23.26701710228813	31.948400853524273	30.147004260049282	14.637577784138319
6	22.658638752970145	40.875195548831556	26.3926181357249	10.073547562473403
7	89.22073506673269	3.0473436404415426	6.541666855968536	1.1902544368572254
8	89.51521305546848	2.4981410556380914	6.486390709972876	1.5002551789205554
9	84.20983885110424	5.186265467855785	8.068386436293864	2.5355092447461156
10	40.621455322487094	33.21903286431902	14.500409649246352	11.659102163947535
11	32.8074148785212	24.72377071151758	25.965628838095412	16.503185571865806
12	30.65331104114514	22.40879814514456	28.930247561413314	18.00764325229699
13	23.881377390693316	27.785804177059337	29.851314739346467	18.481503692900876
14	20.578324784460893	29.200494910808533	30.381019231555562	19.840161073175018
15	19.1662464165156	25.025707193925378	36.89921265566293	18.908833733896092
16	23.29351936406687	23.996056463447324	33.87962066934113	18.830803503144683
17	22.680408468002682	23.824662550486806	29.568687047663182	23.926241933847326
18	23.358525626172725	24.440840136842535	31.74766515073729	20.452969086247446
19	26.06690533846417	25.627990023034254	27.915021633417687	20.39008300508389
20	27.591694039717048	25.68356905202167	27.87894069702463	18.845796211236657
21	25.75663957378305	26.099048795964386	29.73951305501415	18.404798575238406
22	25.729152942281104	23.99427702587075	28.088914331060195	22.18765570078795
23	22.990295628944104	26.635606015861985	29.088352483110487	21.285745872083425
24	21.61270806168515	25.309092093088058	33.261701505782796	19.816498339443996
25	22.301577566062566	26.155422892805163	30.66777370400154	20.875225837130728
26	21.447182506689927	28.714367709038484	28.174251613987742	21.664198170283846
27	21.203929603935055	28.594161021684904	29.388017343080108	20.813892031299925
28	19.822934603018837	28.25852880644414	31.608111812285237	20.31042477825179
29	21.139869851178442	25.63310117352015	31.652067706463978	21.574961268837427
30	21.763770953823975	27.241788463489726	30.966719216865734	20.027721365820565
31	24.493806800026046	26.86912880250666	28.36147116326756	20.27559323419973
32	25.65165275676527	26.131116532716664	27.9935440490307	20.223686661487367
33	24.29231388975968	26.92705517468019	28.02315086147495	20.75748007408518
34	22.08346395162353	26.860913101355248	29.582543944536067	21.473079002485154
35	22.51204338495974	27.144638743883657	29.50561166462978	20.837706206526825
36	25.17907956887635	27.31277666468278	27.3743376327574	20.133806133683464
37	22.61748452646516	28.831697007970362	28.379038376789474	20.171780088775005
38	22.743748873653875	28.76597139875909	27.327504350156968	21.16277537743007
39	22.23687418694847	28.64228155700031	27.801440511508797	21.319403744542427
40	24.421720647987872	26.265142256569153	28.048062987546967	21.26507410789601
41	21.1389233418292	25.66766769495442	29.236121522713955	23.957287440502423
42	22.35019028623957	26.194570519489762	28.02103068053265	23.434208513738014
43	21.808635496977985	26.738889116051133	28.728451768155182	22.724023618815696
44	21.520972375556738	27.469897216656747	28.916049921174704	22.093080486611814
45	20.96219111613897	29.349664784248873	27.043816568002534	22.644327531609626
46	21.440254058253487	29.057306976455386	27.938457204904886	21.563981760386238
47	21.319593046412276	27.45834980259601	29.315704028798116	21.9063531221936
48	21.98832083183785	26.663092647363936	30.001279680640174	21.347306840158044
49	21.993545563445657	26.810823826593428	29.7358405987391	21.459790011221816
50	20.950492260582354	28.38388450465758	29.356744674181197	21.30887856057887
51	20.811847571105567	28.69649761252482	28.548425689929598	21.94322912644002
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2688.0
1	3971.0
2	5254.0
3	19165.0
4	33076.0
5	22526.0
6	11976.0
7	12328.0
8	12680.0
9	13869.0
10	15058.0
11	15510.5
12	15963.0
13	15721.0
14	15479.0
15	15027.0
16	14575.0
17	13662.0
18	12749.0
19	12313.5
20	11878.0
21	12650.0
22	13422.0
23	13762.0
24	14102.0
25	17245.0
26	24254.0
27	28120.0
28	34867.0
29	41614.0
30	46512.0
31	51410.0
32	60074.0
33	68738.0
34	75342.0
35	81946.0
36	89114.5
37	96283.0
38	105592.0
39	114901.0
40	127057.0
41	139213.0
42	152594.5
43	165976.0
44	171966.0
45	177956.0
46	182914.0
47	187872.0
48	186092.0
49	184312.0
50	178737.5
51	173163.0
52	166990.0
53	160817.0
54	150712.5
55	140608.0
56	134516.0
57	128424.0
58	126385.0
59	124346.0
60	110713.0
61	97080.0
62	87388.0
63	77696.0
64	69737.0
65	61778.0
66	53345.0
67	44912.0
68	38466.0
69	32020.0
70	27003.5
71	21987.0
72	19100.5
73	16214.0
74	13410.5
75	9022.5
76	7438.0
77	5423.0
78	3408.0
79	2444.5
80	1481.0
81	1220.5
82	960.0
83	643.0
84	326.0
85	283.5
86	241.0
87	164.5
88	88.0
89	60.0
90	32.0
91	23.0
92	14.0
93	12.5
94	11.0
95	7.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2641284.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.07426722033748
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.00010857377494	15.450177158125323
2	6.012446380946772	2.293660178362594
3	2.0249401660505404	1.1587274949732769
4	1.117281488942771	0.852453027217238
5	0.7496662279997305	0.7149666979464653
6	0.5348139688605074	0.6120710733128739
7	0.4451427365970146	0.594354005633063
8	0.35281966138456217	0.5383821201470499
9	0.30504030141111427	0.5236578199879094
>10	4.337774813000458	21.154569160192967
>50	2.083296859862623	28.49429818743025
>100	1.033460564327882	26.27706071810009
>500	0.002005160525666264	0.24772560970371618
>1k	0.001002580262833132	0.2801207913339536
>5k	0.0	0.0
>10k+	2.005160525666264E-4	0.8077759575331921
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21120	0.799611098238584	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.786037396962992E-5	0.0	0.0	0.01855158324511866	0.0
2	3.786037396962992E-5	0.0	0.0	0.0726161972737502	0.0
3	3.786037396962992E-5	0.0	0.0	0.10949220152016974	0.0
4	3.786037396962992E-5	0.0	0.0	0.17756515391756433	0.0
5	3.786037396962992E-5	0.0	0.0	0.2963331470602934	0.0
6	3.786037396962992E-5	0.0	0.0	0.4986589855539957	0.0
7	3.786037396962992E-5	0.0	0.0	0.5991025576954239	0.0
8	3.786037396962992E-5	0.0	0.0	0.7968851513127706	0.0
9	3.786037396962992E-5	0.0	0.0	0.8877121884659128	0.0
10	3.786037396962992E-5	0.0	0.0	1.0393429862142807	0.0
11	3.786037396962992E-5	0.0	0.0	1.2242151923079836	0.0
12	3.786037396962992E-5	0.0	0.0	1.3669488021734884	0.0
13	3.786037396962992E-5	0.0	0.0	1.4400950446828134	0.0
14	3.786037396962992E-5	0.0	0.0	1.478485463888018	0.0
15	3.786037396962992E-5	0.0	0.0	1.5235014485379081	0.0
16	3.786037396962992E-5	0.0	0.0	1.6028567923782524	0.0
17	3.786037396962992E-5	0.0	0.0	1.6929644824259715	0.0
18	3.786037396962992E-5	0.0	0.0	1.803213891425534	0.0
19	3.786037396962992E-5	0.0	0.0	1.8712868438229286	0.0
20	3.786037396962992E-5	0.0	0.0	1.9450767126897373	0.0
21	3.786037396962992E-5	0.0	0.0	2.041658526686263	0.0
22	3.786037396962992E-5	0.0	0.0	2.1428214459331145	0.0
23	3.786037396962992E-5	0.0	0.0	2.2544338283955834	0.0
24	3.786037396962992E-5	0.0	0.0	2.3490090425717187	0.0
25	3.786037396962992E-5	0.0	0.0	2.433929861385599	0.0
26	3.786037396962992E-5	0.0	0.0	2.5095370282029497	0.0
27	7.572074793925984E-5	0.0	0.0	2.5960858430975238	0.0
28	7.572074793925984E-5	0.0	0.0	2.6785457376033777	0.0
29	7.572074793925984E-5	0.0	0.0	2.7764526646888408	0.0
30	7.572074793925984E-5	0.0	0.0	2.909001833956515	0.0
31	7.572074793925984E-5	0.0	0.0	3.017017480891869	0.0
32	7.572074793925984E-5	0.0	0.0	3.1220421582836226	0.0
33	7.572074793925984E-5	0.0	0.0	3.2265367904398015	0.0
34	7.572074793925984E-5	0.0	0.0	3.339095682251511	0.0
35	7.572074793925984E-5	0.0	0.0	3.4687674630974934	0.0
36	7.572074793925984E-5	0.0	0.0	3.579509056958661	0.0
37	7.572074793925984E-5	0.0	0.0	3.6889255377308916	0.0
38	7.572074793925984E-5	0.0	0.0	3.822194054103989	0.0
39	7.572074793925984E-5	0.0	0.0	4.02868453373435	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGAC	580	0.0	40.344826	5
CGTTTTT	10685	0.0	39.88301	1
TGCGATA	40	3.4605E-7	39.375	1
GGGCGAT	4715	0.0	38.748676	7
CGTAAGC	35	6.251372E-6	38.571426	37
CGTCGAA	35	6.251372E-6	38.571426	31
AGGGCGA	2630	0.0	38.070343	6
TTAGGGA	2915	0.0	37.59005	4
TAAGGGA	3400	0.0	37.588238	4
TAACGCC	90	0.0	37.5	12
CGAATAT	210	0.0	37.5	14
TAGTACG	90	0.0	37.5	1
AGGGATT	3905	0.0	37.33675	6
CGTTAGG	315	0.0	37.142857	2
TATCACG	85	0.0	37.058823	1
CATACGA	940	0.0	36.861702	18
TACGGGA	915	0.0	36.639343	4
TAACGGG	740	0.0	36.486485	3
AGTAGGG	3840	0.0	36.210938	3
GTTTACG	100	0.0	36.000004	1
>>END_MODULE