FastQCFastQC Report
Thu 26 May 2016
SRR1547302_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547302_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2043531
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT509822.494799442729276No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG207.034575E-445.0000041
CCGCCTA207.034575E-445.00000420
TAATGCG302.1663345E-645.0000041
CGTTTTT230800.044.1421131
GCGATAC750.042.09
CGTAAGG3400.041.6911772
AGTCGAG603.6379788E-1241.2500041
AGGGCGA17950.041.114216
AGTCGGC556.184564E-1140.9090930
GCGAGAC5200.040.67307721
CGACGGT950.040.26315728
ACGTAAG1400.040.178571
GTACGAG1400.040.178571
CGCTATA451.929584E-840.011
CGAGGGA14300.039.4930044
TACGGGA11000.039.4772724
ACGTACT1200.039.37500420
GGGCGAT35300.039.199727
AAGGGCG7100.038.9788745
TGTTACG1100.038.8636321
Produced by FastQC (version 0.11.5)