##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547302_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2043531
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.301406731779455	31.0	31.0	33.0	30.0	34.0
2	31.684695754554248	31.0	31.0	34.0	30.0	34.0
3	31.627340128434557	31.0	31.0	34.0	30.0	34.0
4	35.479373202559685	37.0	35.0	37.0	33.0	37.0
5	35.42553648562219	37.0	35.0	37.0	33.0	37.0
6	35.4889522106589	37.0	35.0	37.0	33.0	37.0
7	35.94878864083785	37.0	35.0	37.0	35.0	37.0
8	35.96669392341002	37.0	35.0	37.0	35.0	37.0
9	37.70820702010393	39.0	37.0	39.0	35.0	39.0
10	37.00340978433897	39.0	37.0	39.0	32.0	39.0
11	36.686805338406906	39.0	35.0	39.0	32.0	39.0
12	36.500925114422046	39.0	35.0	39.0	32.0	39.0
13	36.39857090496792	39.0	35.0	39.0	32.0	39.0
14	37.53170566044753	40.0	36.0	41.0	32.0	41.0
15	37.65536514983135	40.0	36.0	41.0	32.0	41.0
16	37.71901527307391	40.0	36.0	41.0	32.0	41.0
17	37.61574353410836	40.0	36.0	41.0	32.0	41.0
18	37.396405045971896	39.0	36.0	41.0	32.0	41.0
19	37.22378324576432	38.0	36.0	41.0	32.0	41.0
20	36.980428483835084	38.0	35.0	41.0	32.0	41.0
21	36.85884481321791	38.0	35.0	40.0	31.0	41.0
22	36.779296227950546	38.0	35.0	40.0	31.0	41.0
23	36.68412713093171	38.0	35.0	40.0	31.0	41.0
24	36.56332984427444	38.0	35.0	40.0	31.0	41.0
25	36.46630464622264	38.0	35.0	40.0	31.0	41.0
26	36.26829345872414	38.0	35.0	40.0	31.0	41.0
27	36.225806214831096	38.0	35.0	40.0	30.0	41.0
28	36.17869315415328	38.0	35.0	40.0	30.0	41.0
29	36.11254392519614	38.0	35.0	40.0	30.0	41.0
30	36.01587203717487	38.0	35.0	40.0	30.0	41.0
31	35.85355788583584	38.0	35.0	40.0	30.0	41.0
32	35.54430982451453	38.0	34.0	40.0	28.0	41.0
33	35.15919993384001	38.0	34.0	40.0	25.0	41.0
34	34.758498892358375	38.0	34.0	40.0	23.0	41.0
35	34.46108084487096	38.0	33.0	40.0	22.0	41.0
36	34.1820750455951	38.0	33.0	40.0	20.0	41.0
37	34.10249416328894	38.0	33.0	40.0	20.0	41.0
38	34.02122649472898	37.0	33.0	40.0	19.0	41.0
39	33.888122078891875	37.0	33.0	40.0	18.0	41.0
40	33.77992112671645	37.0	33.0	40.0	18.0	41.0
41	33.72934934679239	37.0	33.0	40.0	18.0	41.0
42	33.63899984879114	37.0	33.0	40.0	18.0	41.0
43	33.532984818923715	37.0	33.0	40.0	18.0	41.0
44	33.4396008673223	37.0	33.0	40.0	17.0	41.0
45	33.29428083058197	37.0	32.0	40.0	17.0	41.0
46	33.214120069624585	36.0	32.0	40.0	17.0	41.0
47	33.171463021603294	36.0	32.0	40.0	17.0	41.0
48	33.048637383039456	36.0	32.0	40.0	15.0	41.0
49	32.9178637368359	36.0	32.0	40.0	15.0	41.0
50	32.78746003853134	36.0	31.0	40.0	15.0	41.0
51	32.37846306221927	35.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	0.0
12	3.0
13	4.0
14	21.0
15	78.0
16	196.0
17	458.0
18	1141.0
19	2174.0
20	3849.0
21	6270.0
22	9939.0
23	15074.0
24	22775.0
25	34646.0
26	48948.0
27	54517.0
28	53480.0
29	52616.0
30	57228.0
31	65668.0
32	78705.0
33	98412.0
34	142090.0
35	191378.0
36	182722.0
37	206606.0
38	280411.0
39	434010.0
40	109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.592455411735862	22.49615983315154	27.50371782958027	20.407666925532325
2	36.397881901473475	23.874215756942274	27.45943173849577	12.268470603088478
3	25.957472629482986	23.843973984245896	38.004121297890755	12.194432088380356
4	22.398534693136536	26.49888844358123	37.824628058003526	13.277948805278704
5	20.619701878757894	30.672057336052156	35.93094501624884	12.777295768941114
6	19.93055157959434	38.25701689869153	32.588299370060945	9.224132151653192
7	83.56555393581012	2.2606459114150947	13.15106059071284	1.0227395620619408
8	84.1360860197374	1.8364781351494057	12.880254813849165	1.147181031264023
9	79.42683521806129	5.019889593062205	13.956529164470712	1.5967460244057956
10	33.130253468139216	38.11275679204279	19.628280657352395	9.128709082465596
11	22.867428974652206	25.43778391421515	35.983060692497446	15.711726418635196
12	23.64270471062098	23.71821127254737	35.71015071462092	16.928933302210734
13	19.658424560234224	27.628403973318733	36.74116027601245	15.972011190434596
14	17.003020751826128	30.0573859657622	36.246917712527974	16.692675569883697
15	15.761150674983643	27.98352459541842	40.7198129120625	15.535511817535433
16	18.899248408759153	26.356928277574454	39.814957541627706	14.92886577203869
17	18.88725935647661	25.815952877641692	35.85338318821687	19.443404577664836
18	18.993692779801236	27.16415850799425	36.8972626302219	16.944886081982606
19	20.981722322783455	28.53076366348247	33.7295592775446	16.75795473618947
20	22.195601632664246	28.567807388290174	33.6178408842342	15.618750094811382
21	20.385988761609195	28.480409643895786	35.518179073378384	15.615422521116637
22	20.211242207727704	26.264979586803427	34.82036729562703	18.70341090984184
23	17.530441182443525	29.486364532762167	34.94329178270357	18.039902502090744
24	17.232133987690915	28.90951984579632	37.844397760542904	16.013948405969863
25	17.78475589555529	29.747187588541596	35.639195099071166	16.828861416831945
26	17.372723976293976	31.980429951882307	33.28674730160688	17.360098770216844
27	16.706524148642718	31.334684915472288	34.66113310735193	17.297657828533065
28	15.963936930734107	30.658600236551344	36.851508491919134	16.52595434079542
29	17.193866890201324	28.024580982622727	37.056447883589726	17.725104243586223
30	18.50375648815702	29.783056875574683	35.26675151979588	16.44643511647242
31	19.939310927996686	29.879458642907792	33.2794560004228	16.901774428672724
32	20.763130091982944	29.60444446401841	33.121053705571384	16.51137173842726
33	20.198714871465125	30.400077121413865	31.936926819314216	17.46428118780679
34	18.083503504473384	31.16287445602734	32.71567693369956	18.037945105799714
35	17.875334408922594	31.06608120943602	32.56730629483967	18.491278086801717
36	20.02661080257652	31.340948583603577	30.475632618247534	18.15680799557237
37	18.113109123375175	32.86306887441394	31.493527624489182	17.5302943777217
38	18.068774097383404	33.25136736364655	30.34238286573583	18.33747567323422
39	18.436128446302014	32.74909947536886	30.320704701812694	18.494067376516433
40	20.067422515244445	31.601918444104836	30.097023240655513	18.233635799995206
41	17.491440061344797	31.38249431988064	30.69520354719356	20.430862071581004
42	18.8282927932094	31.907516940041525	29.523506127384415	19.74068413936466
43	18.4581002196688	31.655306427942616	30.319236654594423	19.56735669779416
44	18.664458723650387	32.15434461234011	30.292811804665554	18.888384859343947
45	17.939488072361026	33.26820097174939	29.158207044571384	19.634103911318203
46	19.37078517526771	32.9235524198067	28.84786186262895	18.857800542296644
47	18.449389806173727	32.11509881670501	30.487230191271873	18.948281185849396
48	18.691715467002947	32.03142012526358	30.595131661814772	18.681732745918705
49	18.851781548701734	30.85350797223042	31.021501508907868	19.273208970159985
50	17.71096205538355	32.6260281835705	31.076797954129397	18.58621180691656
51	17.630855612173242	32.87902165418582	29.898102842579828	19.59201989106111
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1735.0
1	2131.5
2	2528.0
3	35994.5
4	69461.0
5	45704.0
6	21947.0
7	22652.5
8	23358.0
9	25988.0
10	28618.0
11	31050.0
12	33482.0
13	34422.5
14	35363.0
15	33643.0
16	31923.0
17	29981.0
18	28039.0
19	25803.5
20	23568.0
21	22879.0
22	22190.0
23	22986.0
24	23782.0
25	24515.5
26	29880.0
27	34511.0
28	39162.0
29	43813.0
30	47979.0
31	52145.0
32	59119.5
33	66094.0
34	72619.0
35	79144.0
36	83483.5
37	87823.0
38	96688.5
39	105554.0
40	116701.0
41	127848.0
42	131252.0
43	134656.0
44	135876.5
45	137097.0
46	137609.0
47	138121.0
48	139616.0
49	141111.0
50	132541.5
51	123972.0
52	116187.0
53	108402.0
54	96643.0
55	84884.0
56	73400.0
57	61916.0
58	55305.0
59	48694.0
60	41667.0
61	34640.0
62	28540.0
63	22440.0
64	18397.0
65	14354.0
66	11666.5
67	8979.0
68	7280.0
69	5581.0
70	4401.0
71	3221.0
72	3047.5
73	2874.0
74	2351.0
75	1598.5
76	1369.0
77	1070.0
78	771.0
79	494.0
80	217.0
81	176.5
82	136.0
83	89.5
84	43.0
85	33.5
86	24.0
87	19.0
88	14.0
89	10.5
90	7.0
91	5.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2043531.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.390818196912853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.34079636599685	21.184276924654778
2	7.372057026008822	4.038533474733634
3	3.2379587558239136	2.6607101882962487
4	2.0073599164597336	2.19932922110075
5	1.3248634367029883	1.8144546765234355
6	0.9122658776744428	1.4992625281576681
7	0.6572443840219042	1.2601723003580168
8	0.520517423622169	1.1405918495008245
9	0.437047599745483	1.0773982213223094
>10	4.800162383144859	30.408643853744373
>50	1.192130028061209	22.59647863073167
>100	0.19470163282275105	6.973035494127235
>500	0.00199042931648052	0.3694438728495597
>1k	7.23792478720189E-4	0.2508392058615001
>5k	0.0	0.0
>10k+	1.8094811968004726E-4	2.526829558038134
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50982	2.494799442729276	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.8934907275690946E-5	0.0	0.0	0.03738626915862788	0.0
2	4.8934907275690946E-5	0.0	0.0	0.11005460646302895	0.0
3	4.8934907275690946E-5	0.0	0.0	0.18017832858909408	0.0
4	4.8934907275690946E-5	0.0	0.0	0.26855477112899195	0.0
5	4.8934907275690946E-5	0.0	0.0	0.4821067064801072	0.0
6	4.8934907275690946E-5	0.0	0.0	0.8956556078669714	0.0
7	4.8934907275690946E-5	0.0	0.0	1.168859195187154	0.0
8	4.8934907275690946E-5	0.0	0.0	1.5924886874728106	0.0
9	4.8934907275690946E-5	0.0	0.0	1.803789617089244	0.0
10	4.8934907275690946E-5	0.0	0.0	2.0446961656074705	0.0
11	4.8934907275690946E-5	0.0	0.0	2.2717541353666766	0.0
12	4.8934907275690946E-5	0.0	0.0	2.456630215054237	0.0
13	4.8934907275690946E-5	0.0	0.0	2.5774015662106424	0.0
14	4.8934907275690946E-5	0.0	0.0	2.6450296080656472	0.0
15	4.8934907275690946E-5	0.0	0.0	2.7055131534584014	0.0
16	4.8934907275690946E-5	0.0	0.0	2.804312731248021	0.0
17	4.8934907275690946E-5	0.0	0.0	2.9307605316484064	0.0
18	4.8934907275690946E-5	0.0	0.0	3.092049986029084	0.0
19	4.8934907275690946E-5	0.0	0.0	3.192219741222423	0.0
20	4.8934907275690946E-5	0.0	0.0	3.289159792535567	0.0
21	4.8934907275690946E-5	0.0	0.0	3.4194734506107323	0.0
22	4.8934907275690946E-5	0.0	0.0	3.5660334979014263	0.0
23	4.8934907275690946E-5	0.0	0.0	3.7240443134946326	0.0
24	4.8934907275690946E-5	0.0	0.0	3.851911226206013	0.0
25	4.8934907275690946E-5	0.0	0.0	3.9718017490314557	0.0
26	4.8934907275690946E-5	0.0	0.0	4.080339373368939	0.0
27	4.8934907275690946E-5	0.0	0.0	4.196412973426877	0.0
28	4.8934907275690946E-5	0.0	0.0	4.326384087151112	0.0
29	4.8934907275690946E-5	0.0	0.0	4.466044312515934	0.0
30	4.8934907275690946E-5	0.0	0.0	4.634869742617068	0.0
31	4.8934907275690946E-5	0.0	0.0	4.787448783502673	0.0
32	4.8934907275690946E-5	0.0	0.0	4.938608712077282	0.0
33	4.8934907275690946E-5	0.0	0.0	5.086587871678971	0.0
34	4.8934907275690946E-5	0.0	0.0	5.246702888285032	0.0
35	4.8934907275690946E-5	0.0	0.0	5.416898495789885	0.0
36	4.8934907275690946E-5	0.0	0.0	5.579704932296109	0.0
37	4.8934907275690946E-5	0.0	0.0	5.7479920784172105	0.0
38	4.8934907275690946E-5	0.0	0.0	5.924010939887871	0.0
39	4.8934907275690946E-5	0.0	0.0	6.138296898848121	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	20	7.034575E-4	45.000004	1
CCGCCTA	20	7.034575E-4	45.000004	20
TAATGCG	30	2.1663345E-6	45.000004	1
CGTTTTT	23080	0.0	44.142113	1
GCGATAC	75	0.0	42.0	9
CGTAAGG	340	0.0	41.691177	2
AGTCGAG	60	3.6379788E-12	41.250004	1
AGGGCGA	1795	0.0	41.11421	6
AGTCGGC	55	6.184564E-11	40.90909	30
GCGAGAC	520	0.0	40.673077	21
CGACGGT	95	0.0	40.263157	28
ACGTAAG	140	0.0	40.17857	1
GTACGAG	140	0.0	40.17857	1
CGCTATA	45	1.929584E-8	40.0	11
CGAGGGA	1430	0.0	39.493004	4
TACGGGA	1100	0.0	39.477272	4
ACGTACT	120	0.0	39.375004	20
GGGCGAT	3530	0.0	39.19972	7
AAGGGCG	710	0.0	38.978874	5
TGTTACG	110	0.0	38.863632	1
>>END_MODULE