FastQCFastQC Report
Thu 26 May 2016
SRR1547301_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547301_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1378390
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290142.104919507541407No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG207.0337765E-445.01
CCGTCGA207.0337765E-445.036
ATCGTAC207.0337765E-445.036
GTATCGC253.8912112E-545.041
TCGTACT207.0337765E-445.037
TATACGC207.0337765E-445.033
TCGGTAT207.0337765E-445.037
CCCGTAA302.165818E-644.99999620
CGCGTAG302.165818E-644.9999961
CGTTTTT95800.043.1210861
TACGGGT603.6379788E-1241.2499964
CACGACG1050.040.71428326
AATCGGT501.0822987E-940.526
CGAATAT451.9288564E-840.014
TACCGGG1750.039.8571433
ACGGGTA1250.039.6000025
TTACGGG2850.039.4736863
TAATGCG800.039.3751
CGAGGGT2650.039.0566024
ATAGGGA14350.038.728224
Produced by FastQC (version 0.11.5)