##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547301_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1378390
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.248374552920435	31.0	31.0	33.0	30.0	34.0
2	31.64376772901719	31.0	31.0	34.0	30.0	34.0
3	31.663313721080392	31.0	31.0	34.0	30.0	34.0
4	35.48548306357417	37.0	35.0	37.0	33.0	37.0
5	35.401237675839205	37.0	35.0	37.0	33.0	37.0
6	35.44278614905796	37.0	35.0	37.0	33.0	37.0
7	35.87291840480561	37.0	35.0	37.0	35.0	37.0
8	35.87121787012384	37.0	35.0	37.0	35.0	37.0
9	37.59382467951741	39.0	37.0	39.0	35.0	39.0
10	36.90505299661199	39.0	37.0	39.0	32.0	39.0
11	36.59362879881601	39.0	35.0	39.0	32.0	39.0
12	36.19492161144524	38.0	35.0	39.0	32.0	39.0
13	36.01019014937717	38.0	35.0	39.0	31.0	39.0
14	37.09501592437554	39.0	35.0	41.0	31.0	41.0
15	37.26635640130878	39.0	35.0	41.0	32.0	41.0
16	37.36455067143552	39.0	35.0	41.0	32.0	41.0
17	37.27095742133939	39.0	35.0	41.0	32.0	41.0
18	37.09264721885678	39.0	35.0	41.0	32.0	41.0
19	36.98662062261044	38.0	35.0	41.0	31.0	41.0
20	36.794980375655655	38.0	35.0	40.0	31.0	41.0
21	36.642879736504185	38.0	35.0	40.0	31.0	41.0
22	36.53446847408934	38.0	35.0	40.0	31.0	41.0
23	36.43541813274908	38.0	35.0	40.0	31.0	41.0
24	36.29987521673837	38.0	35.0	40.0	30.0	41.0
25	36.1184410798105	38.0	35.0	40.0	30.0	41.0
26	35.88040322405125	38.0	35.0	40.0	30.0	41.0
27	35.81396701949376	37.0	34.0	40.0	30.0	41.0
28	35.78367878466907	37.0	34.0	40.0	30.0	41.0
29	35.7538374480372	37.0	34.0	40.0	30.0	41.0
30	35.675421324879025	37.0	34.0	40.0	29.0	41.0
31	35.539807311428554	37.0	34.0	40.0	29.0	41.0
32	35.31101502477528	37.0	34.0	40.0	27.0	41.0
33	35.05831948867882	37.0	34.0	40.0	26.0	41.0
34	34.779088646899645	37.0	34.0	40.0	24.0	41.0
35	34.589048817823695	37.0	33.0	40.0	24.0	41.0
36	34.320140889008194	37.0	33.0	40.0	23.0	41.0
37	34.24906158634349	36.0	33.0	40.0	23.0	41.0
38	34.155337023629016	36.0	33.0	40.0	23.0	41.0
39	34.019030898366935	36.0	33.0	40.0	22.0	41.0
40	33.88434768099014	36.0	33.0	40.0	22.0	41.0
41	33.83968543010324	36.0	33.0	40.0	22.0	41.0
42	33.73352099188183	36.0	33.0	40.0	21.0	41.0
43	33.601610574655936	35.0	33.0	40.0	21.0	41.0
44	33.49377824853634	35.0	33.0	40.0	21.0	41.0
45	33.32840850557535	35.0	32.0	40.0	20.0	41.0
46	33.24207299820805	35.0	32.0	40.0	20.0	41.0
47	33.17952103541088	35.0	32.0	39.0	20.0	41.0
48	33.03847749911128	35.0	32.0	39.0	20.0	41.0
49	32.894241107378896	35.0	32.0	39.0	20.0	41.0
50	32.765060686743226	35.0	31.0	39.0	20.0	41.0
51	32.332142572131254	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	0.0
9	1.0
10	0.0
11	2.0
12	6.0
13	8.0
14	32.0
15	55.0
16	176.0
17	419.0
18	930.0
19	1745.0
20	2992.0
21	4713.0
22	6987.0
23	10392.0
24	14617.0
25	20608.0
26	27324.0
27	31721.0
28	33841.0
29	37204.0
30	41830.0
31	48856.0
32	58721.0
33	73148.0
34	110569.0
35	147994.0
36	134992.0
37	140215.0
38	177934.0
39	250277.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.02780054991693	23.63293407526172	26.26847263836795	18.0707927364534
2	34.23646428079136	24.77049311152867	28.077612286798363	12.915430320881608
3	27.732426961890322	24.989734400278586	33.97021162370592	13.307627014125176
4	24.397957036832825	27.884923715349068	33.75713694962964	13.959982298188468
5	21.712940459521615	31.85840001741162	32.68537931935084	13.74328020371593
6	21.312835989814204	40.61492030557389	28.731636184243936	9.340607520367966
7	85.52340048897626	3.1374284491326834	9.840828793012138	1.4983422688789094
8	85.63860736076147	2.81589390520825	9.747168798380718	1.7983299356495623
9	80.52430734407532	5.327809981209962	11.409107727130928	2.7387749475837753
10	41.83503943005971	29.361936752297975	17.267464215497792	11.53555960214453
11	32.33932341354769	25.485167477999692	26.15827160672959	16.017237501723024
12	29.796211522138144	22.868418952546087	29.26820420925863	18.06716531605714
13	22.67398921930658	28.743534123143665	30.42941402650919	18.153062631040562
14	18.807521818933683	31.611880527281826	31.094465281959387	18.4861323718251
15	18.424683870312467	27.43918629705671	36.95187864102322	17.1842511916076
16	21.2831636909728	25.101241303259602	36.00802385391653	17.607571151851072
17	20.955535080782653	26.04400786424742	29.485196497362864	23.51526055760706
18	21.860141179201822	27.887317812810597	31.31392421593308	18.9386167920545
19	25.020422376830943	28.995784937499547	27.58065569251083	18.403136993158686
20	26.43090852371245	28.181356510131383	27.21863913696414	18.169095829192027
21	24.31800869129927	28.862658608956828	29.124413264750903	17.694919434992997
22	23.474053061905558	27.189619773794067	28.276612569737157	21.05971459456322
23	21.52380676006065	29.836403340128705	28.015801043246107	20.623988856564544
24	21.719252171011107	28.046126277758837	31.787810416500413	18.44681113472965
25	21.497761881615506	28.915038559478813	30.188408215381713	19.398791343523968
26	20.10555793353115	31.65345076502296	28.275234149986577	19.96575715145931
27	20.145241912666226	31.52119501737534	29.07319408875572	19.260368981202706
28	19.319060643214183	30.945813594120676	31.35883168043877	18.376294082226366
29	20.200306154281446	28.780969101633065	31.279608818984467	19.73911592510102
30	21.376388395156667	30.332489353521137	29.766539223296746	18.52458302802545
31	23.479494192499946	30.214888384274406	27.285891511110787	19.01972591211486
32	24.65934895058728	29.80462713745747	27.009699722139597	18.526324189815654
33	23.724562714471233	30.33394032167964	26.87417929613535	19.06731766771378
34	20.523509311588157	31.535632150552455	27.82093601955905	20.11992251830034
35	21.274530430429706	30.528660248550842	28.2741459238677	19.922663397151748
36	23.683935606033128	31.051444076059752	26.07012529110049	19.19449502680664
37	21.358323841583296	32.36188596841242	26.92075537402332	19.359034815980962
38	20.870508346694333	32.917751869935216	25.768396462539627	20.443343320830827
39	20.740501599692397	32.26336523045002	26.268907928815505	20.727225241042085
40	22.674134316122434	30.345185324908048	26.761293973403756	20.21938638556577
41	20.05883675882733	29.685357554828464	27.622080833436108	22.633724852908102
42	21.37471978177439	30.458215744455487	26.331734850078714	21.835329623691408
43	21.163894108343793	30.331763869441886	26.911977016664366	21.592365005549954
44	20.620724178207908	31.384586365252215	27.057509122962298	20.93718033357758
45	19.811446687802437	33.13779119117231	25.85509181001023	21.195670311015025
46	21.435007508760222	32.19059917730105	26.39775390129064	19.97663941264809
47	21.014154194386204	30.49993107901247	27.683964625396296	20.80195010120503
48	21.713593395192945	29.73084540659755	28.4368720028439	20.118689195365608
49	20.749860344314744	30.227729452477163	27.789304913703667	21.233105289504422
50	19.90242239134062	31.886693896502443	27.93977031174051	20.271113400416425
51	19.784676325278042	32.70257329202911	26.419953714115746	21.092796668577108
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1468.0
1	1954.5
2	2441.0
3	19559.5
4	36678.0
5	23002.5
6	9327.0
7	10499.5
8	11672.0
9	14465.0
10	17258.0
11	19893.5
12	22529.0
13	22541.5
14	22554.0
15	21137.5
16	19721.0
17	17724.5
18	15728.0
19	13919.0
20	12110.0
21	11507.5
22	10905.0
23	10539.5
24	10174.0
25	11037.0
26	12922.5
27	13945.0
28	16592.0
29	19239.0
30	20318.0
31	21397.0
32	23935.5
33	26474.0
34	30026.5
35	33579.0
36	36800.5
37	40022.0
38	42345.5
39	44669.0
40	54434.0
41	64199.0
42	71261.5
43	78324.0
44	84023.5
45	89723.0
46	91982.0
47	94241.0
48	93690.0
49	93139.0
50	90324.0
51	87509.0
52	80809.0
53	74109.0
54	71040.5
55	67972.0
56	64524.0
57	61076.0
58	59260.5
59	57445.0
60	54652.0
61	51859.0
62	47435.5
63	43012.0
64	38453.5
65	33895.0
66	29956.0
67	26017.0
68	22092.0
69	18167.0
70	15102.0
71	12037.0
72	10717.0
73	9397.0
74	7252.0
75	4077.0
76	3047.0
77	2630.5
78	2214.0
79	1669.0
80	1124.0
81	825.5
82	527.0
83	383.5
84	240.0
85	175.0
86	110.0
87	72.0
88	34.0
89	35.0
90	36.0
91	24.0
92	12.0
93	14.0
94	16.0
95	13.5
96	11.0
97	6.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1378390.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.293680816111387
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.43548589415641	20.623576307575554
2	6.889871461070755	3.623201621228589
3	2.9973238139551612	2.364320269999999
4	1.7614488892608704	1.8525989947247703
5	1.1531979772691963	1.5160909766050759
6	0.7847429147781332	1.238026783432868
7	0.5741224165645729	1.0567054099366393
8	0.4347730727943644	0.9145427522795981
9	0.39070180793849424	0.9245689768991178
>10	4.978967413569438	32.2126975937172
>50	1.4426792108659345	25.940298883708945
>100	0.15418483318530443	5.13473832131833
>500	0.0019446735711026325	0.39941885699729623
>1k	2.778105101575189E-4	0.0798399434341706
>5k	0.0	0.0
>10k+	2.778105101575189E-4	2.119374308141835
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29014	2.104919507541407	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.254840792518808E-5	0.0	0.0	0.06398769579001588	0.0
2	7.254840792518808E-5	0.0	0.0	0.18238669752392284	0.0
3	7.254840792518808E-5	0.0	0.0	0.29962492473102675	0.0
4	7.254840792518808E-5	0.0	0.0	0.41911215258381157	0.0
5	7.254840792518808E-5	0.0	0.0	0.6887745848417356	0.0
6	7.254840792518808E-5	0.0	0.0	1.1948722785278476	0.0
7	7.254840792518808E-5	0.0	0.0	1.5056696580793534	0.0
8	7.254840792518808E-5	0.0	0.0	2.0013203810242386	0.0
9	7.254840792518808E-5	0.0	0.0	2.271490652137639	0.0
10	7.254840792518808E-5	0.0	0.0	2.5981761330247606	0.0
11	7.254840792518808E-5	0.0	0.0	2.9135440622755535	0.0
12	7.254840792518808E-5	0.0	0.0	3.16296548872235	0.0
13	7.254840792518808E-5	0.0	0.0	3.322644534565689	0.0
14	7.254840792518808E-5	0.0	0.0	3.414128076959351	0.0
15	7.254840792518808E-5	0.0	0.0	3.4973411008495416	0.0
16	7.254840792518808E-5	0.0	0.0	3.62205181407294	0.0
17	7.254840792518808E-5	0.0	0.0	3.7563389171424633	0.0
18	7.254840792518808E-5	0.0	0.0	3.9267551273587302	0.0
19	7.254840792518808E-5	0.0	0.0	4.024477832833958	0.0
20	7.254840792518808E-5	0.0	0.0	4.134896509696095	0.0
21	7.254840792518808E-5	0.0	0.0	4.2614209331176225	0.0
22	7.254840792518808E-5	0.0	0.0	4.405719716480822	0.0
23	7.254840792518808E-5	0.0	0.0	4.554008662279906	0.0
24	7.254840792518808E-5	0.0	0.0	4.676397826449699	0.0
25	7.254840792518808E-5	0.0	0.0	4.781738114757071	0.0
26	7.254840792518808E-5	0.0	0.0	4.88337843426026	0.0
27	7.254840792518808E-5	0.0	0.0	4.987630496448755	0.0
28	7.254840792518808E-5	0.0	0.0	5.085353201923984	0.0
29	7.254840792518808E-5	0.0	0.0	5.203824752065816	0.0
30	7.254840792518808E-5	0.0	0.0	5.355813666669085	0.0
31	7.254840792518808E-5	0.0	0.0	5.470004860743331	0.0
32	7.254840792518808E-5	0.0	0.0	5.586590152279109	0.0
33	7.254840792518808E-5	0.0	0.0	5.704844057197165	0.0
34	7.254840792518808E-5	0.0	0.0	5.827523414998658	0.0
35	7.254840792518808E-5	0.0	0.0	5.961592872844405	0.0
36	1.4509681585037616E-4	0.0	0.0	6.087609457410458	0.0
37	1.4509681585037616E-4	0.0	0.0	6.221606366848279	0.0
38	1.4509681585037616E-4	0.0	0.0	6.36046401961709	0.0
39	1.4509681585037616E-4	0.0	0.0	6.530590036201655	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	20	7.0337765E-4	45.0	1
CCGTCGA	20	7.0337765E-4	45.0	36
ATCGTAC	20	7.0337765E-4	45.0	36
GTATCGC	25	3.8912112E-5	45.0	41
TCGTACT	20	7.0337765E-4	45.0	37
TATACGC	20	7.0337765E-4	45.0	33
TCGGTAT	20	7.0337765E-4	45.0	37
CCCGTAA	30	2.165818E-6	44.999996	20
CGCGTAG	30	2.165818E-6	44.999996	1
CGTTTTT	9580	0.0	43.121086	1
TACGGGT	60	3.6379788E-12	41.249996	4
CACGACG	105	0.0	40.714283	26
AATCGGT	50	1.0822987E-9	40.5	26
CGAATAT	45	1.9288564E-8	40.0	14
TACCGGG	175	0.0	39.857143	3
ACGGGTA	125	0.0	39.600002	5
TTACGGG	285	0.0	39.473686	3
TAATGCG	80	0.0	39.375	1
CGAGGGT	265	0.0	39.056602	4
ATAGGGA	1435	0.0	38.72822	4
>>END_MODULE