FastQCFastQC Report
Thu 26 May 2016
SRR1547300_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547300_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1449957
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344512.3760014952167547No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTT14940.10303753835458568No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG207.0338976E-445.040
TAGACGC207.0338976E-445.036
TCGATAG502.1827873E-1145.01
ATACGCG207.0338976E-445.022
CGTTTTT130550.043.0181
ATTAGCG750.042.0000041
ACGTTAG603.6379788E-1241.2500041
TATTGCG1050.040.7142871
ACGTAAG1000.040.51
TGCGACG451.9288564E-840.01
AGGGTAC5550.039.729736
AGGGCGA16650.039.189196
CGTAAGG3050.039.0983622
CGTTAGG1900.039.0789452
GCGATCG1850.038.918929
GACCGAT8500.038.3823559
AGTACGG1700.038.382352
GTTTTTT150500.038.377082
ACGGGAT5100.037.9411775
TAGGGAT13400.037.6119425
Produced by FastQC (version 0.11.5)