##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547300_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1449957
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.309373312449956	31.0	31.0	33.0	30.0	34.0
2	31.70016972917128	31.0	31.0	34.0	30.0	34.0
3	31.688808702602905	31.0	31.0	34.0	30.0	34.0
4	35.527521850647986	37.0	35.0	37.0	33.0	37.0
5	35.45943983166397	37.0	35.0	37.0	33.0	37.0
6	35.50126383058256	37.0	35.0	37.0	33.0	37.0
7	35.91958520149218	37.0	35.0	37.0	35.0	37.0
8	35.92587918124469	37.0	35.0	37.0	35.0	37.0
9	37.65761881214409	39.0	37.0	39.0	35.0	39.0
10	36.960487793775954	39.0	37.0	39.0	32.0	39.0
11	36.68051328418705	39.0	35.0	39.0	32.0	39.0
12	36.37140204847454	38.0	35.0	39.0	32.0	39.0
13	36.265446492551156	39.0	35.0	39.0	32.0	39.0
14	37.38792115904127	39.0	36.0	41.0	32.0	41.0
15	37.49582987633426	39.0	36.0	41.0	32.0	41.0
16	37.57519154016291	40.0	36.0	41.0	32.0	41.0
17	37.48700754574101	39.0	36.0	41.0	32.0	41.0
18	37.25735383876901	39.0	36.0	41.0	32.0	41.0
19	37.11282472514702	38.0	35.0	41.0	32.0	41.0
20	36.902035715541906	38.0	35.0	41.0	32.0	41.0
21	36.78745645560524	38.0	35.0	40.0	31.0	41.0
22	36.67868150572741	38.0	35.0	40.0	31.0	41.0
23	36.57258732500343	38.0	35.0	40.0	31.0	41.0
24	36.42456845271963	38.0	35.0	40.0	31.0	41.0
25	36.283958075998115	38.0	35.0	40.0	30.0	41.0
26	36.05292087972264	38.0	35.0	40.0	30.0	41.0
27	36.00448358123724	38.0	35.0	40.0	30.0	41.0
28	35.96368375062157	38.0	35.0	40.0	30.0	41.0
29	35.92282667692904	38.0	35.0	40.0	30.0	41.0
30	35.8407414840578	38.0	35.0	40.0	30.0	41.0
31	35.72157519154016	38.0	35.0	40.0	29.0	41.0
32	35.45392035763819	38.0	34.0	40.0	27.0	41.0
33	35.15853573588734	38.0	34.0	40.0	26.0	41.0
34	34.83374541451919	37.0	34.0	40.0	24.0	41.0
35	34.58754845833359	37.0	33.0	40.0	23.0	41.0
36	34.328849062420474	37.0	33.0	40.0	22.0	41.0
37	34.27139563449123	37.0	33.0	40.0	22.0	41.0
38	34.174215511218605	37.0	33.0	40.0	22.0	41.0
39	34.025625587517425	37.0	33.0	40.0	21.0	41.0
40	33.905528232906214	37.0	33.0	40.0	21.0	41.0
41	33.84890655378056	36.0	33.0	40.0	21.0	41.0
42	33.743482048088325	36.0	33.0	40.0	20.0	41.0
43	33.613964414117106	36.0	33.0	40.0	20.0	41.0
44	33.50627570334844	36.0	33.0	40.0	19.0	41.0
45	33.357204386061106	35.0	33.0	40.0	20.0	41.0
46	33.267527243911374	35.0	32.0	40.0	20.0	41.0
47	33.214816715254315	35.0	32.0	40.0	20.0	41.0
48	33.06424466380727	35.0	32.0	40.0	18.0	41.0
49	32.92666058372765	35.0	32.0	39.0	18.0	41.0
50	32.80324106163148	35.0	31.0	39.0	18.0	41.0
51	32.36724744251036	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	2.0
12	1.0
13	8.0
14	20.0
15	68.0
16	176.0
17	427.0
18	857.0
19	1699.0
20	2965.0
21	4700.0
22	7171.0
23	10450.0
24	15416.0
25	22967.0
26	30655.0
27	35021.0
28	35721.0
29	37594.0
30	42019.0
31	48827.0
32	58923.0
33	73614.0
34	108291.0
35	144248.0
36	139012.0
37	150364.0
38	195773.0
39	282888.0
40	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.924434310810593	22.740260573244587	26.75396580726187	19.581339308682946
2	36.099691232222746	24.01857434392882	26.703274648834412	13.178459775014018
3	27.395777943759715	24.165682154712172	35.53801940333403	12.900520498194085
4	23.65663257600053	27.281084887344935	35.173387900468775	13.888894636185764
5	21.840578720610335	30.599390188812496	33.656446363581814	13.903584726995351
6	20.57454117604867	39.612140222089344	30.31779563118079	9.4955229706812
7	83.75427685096868	3.0013993518428475	11.665863194563702	1.5784606026247674
8	84.14566776807864	2.5402132614967203	11.54165261452581	1.77246635589883
9	79.0455854897766	5.0514601467491795	13.275773005682238	2.6271813577919896
10	38.92005073253896	28.927478539018743	20.094113135768854	12.058357592673438
11	29.452390657102246	25.599517778803094	28.44967126611341	16.49842029798125
12	26.662652754529965	22.624533003392514	32.38923637045788	18.32357787161964
13	22.18679588429174	26.565477458986713	33.331747079396145	17.9159795773254
14	19.43954200021104	28.577675062088048	33.61658311246472	18.366199825236194
15	18.38447622929508	26.172086482564655	37.88974431655559	17.553692971584674
16	21.014347322024033	25.115227555024045	36.367009504419784	17.503415618532138
17	21.30442488984156	25.888767735870786	31.647904041292257	21.158903332995393
18	21.6908501424525	26.319815001410383	33.57775437478491	18.411580481352203
19	24.068713761856387	28.11724761492927	29.714122556737887	18.099916066476453
20	25.381373378658818	27.625164056589263	29.467218683036805	17.52624388171511
21	23.998780653495242	28.002278688264546	30.49276633720862	17.50617432103159
22	23.30048408332109	26.10946393582706	30.374142129732125	20.215909851119722
23	21.36670259876672	28.585330461524034	30.154687345900605	19.893279593808643
24	21.35642643195626	26.859348242740992	33.71541362950764	18.068811695795116
25	20.935655333227125	28.36215142931825	31.308445698734516	19.393747538720113
26	20.240669206052317	30.440350989719008	30.020062663927277	19.298917140301402
27	19.863278704127087	29.77164150385149	31.011609309793325	19.353470482228094
28	19.535131041817102	29.398733893487876	32.733453474827186	18.33268158986784
29	19.92493570498987	27.847101672670295	32.61186366216378	19.61609896017606
30	20.39253577864723	28.708023755187224	31.862944901124653	19.036495565040894
31	22.988405863070422	28.688367999878615	29.29797228469534	19.025253852355622
32	23.211722830401175	29.357284388433584	29.289627209634496	18.14136557153074
33	22.806193562981523	29.190589789904113	29.155761170848514	18.847455476265846
34	20.33529270178357	30.05309812635823	30.397866971227423	19.213742200630776
35	20.699234529023965	29.892058867952638	30.126548580406176	19.28215802261722
36	22.200451461664038	30.355727790548272	27.93951820640198	19.50430254138571
37	20.745511763452296	31.760941876207365	28.62974557176523	18.863800788575112
38	20.961449201597013	31.3529297765382	27.53543725779454	20.150183764070245
39	20.564885717300584	31.387482525343856	27.471366392244732	20.576265365110828
40	22.354180158446077	29.699708336178244	27.944759741150943	20.001351764224733
41	19.655203568105815	28.943892818890493	29.13231219960316	22.268591413400536
42	20.8095826290021	30.564009829257007	27.308189139402067	21.31821840233883
43	20.66240585065626	29.68212160774423	28.76243916198894	20.893033379610568
44	20.347430992781167	30.91222705225051	28.10724731836875	20.63309463659957
45	20.094733843831232	31.431690732897593	27.750271214939477	20.723304208331694
46	20.897240400922236	30.99533296504655	27.215979508357833	20.89144712567338
47	20.497435441188948	30.529939853388754	28.605951762707445	20.366672942714853
48	21.012623132961874	29.446390479165935	29.19996937840226	20.341017009469937
49	20.470331189131816	29.446252544040963	29.166451143033896	20.916965123793325
50	19.894521009933396	31.067679938094717	29.020929586187727	20.016869465784158
51	19.363677681476073	31.1828557674469	28.282976667583938	21.170489883493097
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1558.0
1	2039.5
2	2521.0
3	24012.5
4	45504.0
5	28909.0
6	12314.0
7	12753.0
8	13192.0
9	15568.5
10	17945.0
11	19852.0
12	21759.0
13	22247.5
14	22736.0
15	21513.5
16	20291.0
17	18737.0
18	17183.0
19	15625.5
20	14068.0
21	13187.5
22	12307.0
23	11876.5
24	11446.0
25	12060.0
26	15084.5
27	17495.0
28	18297.5
29	19100.0
30	21679.0
31	24258.0
32	26972.5
33	29687.0
34	33808.0
35	37929.0
36	42085.0
37	46241.0
38	51412.5
39	56584.0
40	62698.5
41	68813.0
42	76675.5
43	84538.0
44	92456.5
45	100375.0
46	99998.0
47	99621.0
48	96150.5
49	92680.0
50	86854.0
51	81028.0
52	77568.5
53	74109.0
54	70858.0
55	67607.0
56	65141.0
57	62675.0
58	60102.5
59	57530.0
60	54746.0
61	51962.0
62	47557.5
63	43153.0
64	38444.0
65	33735.0
66	29461.0
67	25187.0
68	20902.5
69	16618.0
70	14235.5
71	11853.0
72	10021.5
73	8190.0
74	7018.0
75	4741.5
76	3637.0
77	2820.0
78	2003.0
79	1492.5
80	982.0
81	701.5
82	421.0
83	377.0
84	333.0
85	242.5
86	152.0
87	105.0
88	58.0
89	45.5
90	33.0
91	27.0
92	21.0
93	11.5
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1449957.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.40769896294405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.62082253289307	21.766212608075865
2	7.164311003350429	4.070431805201733
3	3.137575664972413	2.6739391489198594
4	1.891451450885249	2.149271336790876
5	1.2469573021890674	1.7711593830115941
6	0.8803332219121022	1.500494469309457
7	0.7014443741496286	1.394849443406534
8	0.5674838640467014	1.2896728620933569
9	0.4983359473049854	1.2740919816108216
>10	6.451232086316222	43.00637987505706
>50	0.8025846655198423	14.504973544437142
>100	0.034774116906166185	1.558928854699213
>500	0.0012244407064264029	0.24299783130114472
>1k	0.0012244407064264029	0.3999411772545895
>5k	0.0	0.0
>10k+	2.448881412852806E-4	2.396655678830729
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34451	2.3760014952167547	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTT	1494	0.10303753835458568	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05531198511404131	0.0
2	0.0	0.0	0.0	0.15807365321868166	0.0
3	0.0	0.0	0.0	0.24924877082561758	0.0
4	0.0	0.0	0.0	0.36759710805216983	0.0
5	0.0	0.0	0.0	0.6470536712468025	0.0
6	0.0	0.0	0.0	1.2572786641259017	0.0
7	0.0	0.0	0.0	1.6238412587407764	0.0
8	0.0	0.0	0.0	2.173926537131791	0.0
9	0.0	0.0	0.0	2.4964878268803834	0.0
10	0.0	0.0	0.0	2.8487051684981	0.0
11	0.0	0.0	0.0	3.1477485194388524	0.0
12	0.0	0.0	0.0	3.396376582202093	0.0
13	0.0	0.0	0.0	3.559071062107359	0.0
14	0.0	0.0	0.0	3.6569360332754695	0.0
15	0.0	0.0	0.0	3.742662713445985	0.0
16	0.0	0.0	0.0	3.8561833212984937	0.0
17	0.0	0.0	0.0	3.9774972637119586	0.0
18	0.0	0.0	0.0	4.132536344181241	0.0
19	0.0	0.0	0.0	4.229918542411947	0.0
20	6.896756248633581E-5	0.0	0.0	4.329645637767189	0.0
21	1.3793512497267162E-4	0.0	0.0	4.45178719093049	0.0
22	1.3793512497267162E-4	0.0	0.0	4.574135646781249	0.0
23	1.3793512497267162E-4	0.0	0.0	4.725795316688702	0.0
24	1.3793512497267162E-4	0.0	0.0	4.852695631663559	0.0
25	1.3793512497267162E-4	0.0	0.0	4.959871223767325	0.0
26	1.3793512497267162E-4	0.0	0.0	5.071115902057785	0.0
27	1.3793512497267162E-4	0.0	0.0	5.180912261536032	0.0
28	1.3793512497267162E-4	0.0	0.0	5.293743193763677	0.0
29	1.3793512497267162E-4	0.0	0.0	5.418022741364054	0.0
30	1.3793512497267162E-4	0.0	0.0	5.576786070207599	0.0
31	1.3793512497267162E-4	0.0	0.0	5.709755530681255	0.0
32	1.3793512497267162E-4	0.0	0.0	5.849001039341167	0.0
33	1.3793512497267162E-4	0.0	0.0	5.974039230128893	0.0
34	1.3793512497267162E-4	0.0	0.0	6.106319014977686	0.0
35	1.3793512497267162E-4	0.0	0.0	6.243771367012953	0.0
36	1.3793512497267162E-4	0.0	0.0	6.381085783923248	0.0
37	1.3793512497267162E-4	0.0	0.0	6.529779848643788	0.0
38	1.3793512497267162E-4	0.0	0.0	6.684405123738152	0.0
39	1.3793512497267162E-4	0.0	0.0	6.872617601763363	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	20	7.0338976E-4	45.0	40
TAGACGC	20	7.0338976E-4	45.0	36
TCGATAG	50	2.1827873E-11	45.0	1
ATACGCG	20	7.0338976E-4	45.0	22
CGTTTTT	13055	0.0	43.018	1
ATTAGCG	75	0.0	42.000004	1
ACGTTAG	60	3.6379788E-12	41.250004	1
TATTGCG	105	0.0	40.714287	1
ACGTAAG	100	0.0	40.5	1
TGCGACG	45	1.9288564E-8	40.0	1
AGGGTAC	555	0.0	39.72973	6
AGGGCGA	1665	0.0	39.18919	6
CGTAAGG	305	0.0	39.098362	2
CGTTAGG	190	0.0	39.078945	2
GCGATCG	185	0.0	38.91892	9
GACCGAT	850	0.0	38.382355	9
AGTACGG	170	0.0	38.38235	2
GTTTTTT	15050	0.0	38.37708	2
ACGGGAT	510	0.0	37.941177	5
TAGGGAT	1340	0.0	37.611942	5
>>END_MODULE