FastQCFastQC Report
Thu 26 May 2016
SRR1547299_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547299_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2248753
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT444861.9782519467455966No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT255950.043.391291
CGTTAGG2400.043.1249962
TACGGGA6050.040.165294
GTTTACG403.4601908E-739.3750041
GGGCGAT44900.039.2371947
AGGGCGA23050.038.1670276
GTTTTTT297400.038.0926362
GGCGATA9100.038.0769278
GCGTTAG1550.037.7419361
CGTAAGG4300.037.674422
CGCAATA301.14026945E-437.49999643
CGCGTAA301.14026945E-437.49999631
AACACGT10700.037.42990541
CACGACC11650.037.27467727
GACCGAT11950.037.0920529
ACGACCA11800.036.99152828
AGGGATC16400.036.9054876
TATGGGA20150.036.8486374
GGCGATT12400.036.8346758
TTACGCG552.752131E-936.8181841
Produced by FastQC (version 0.11.5)