##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547299_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2248753
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.290642413817793	31.0	31.0	33.0	30.0	34.0
2	31.68251648802692	31.0	31.0	34.0	30.0	34.0
3	31.62933190083571	31.0	31.0	34.0	30.0	34.0
4	35.493450814740434	37.0	35.0	37.0	33.0	37.0
5	35.44320830255702	37.0	35.0	37.0	33.0	37.0
6	35.484862054658734	37.0	35.0	37.0	33.0	37.0
7	35.90179579526964	37.0	35.0	37.0	35.0	37.0
8	35.89998234577119	37.0	35.0	37.0	35.0	37.0
9	37.64295189378291	39.0	37.0	39.0	35.0	39.0
10	36.93187157504626	39.0	37.0	39.0	32.0	39.0
11	36.68696784395618	39.0	35.0	39.0	32.0	39.0
12	36.416798554576694	39.0	35.0	39.0	32.0	39.0
13	36.319066166893386	39.0	35.0	39.0	32.0	39.0
14	37.42260666244803	40.0	36.0	41.0	32.0	41.0
15	37.510554960905	40.0	36.0	41.0	32.0	41.0
16	37.57135310102977	40.0	36.0	41.0	32.0	41.0
17	37.45838071144318	39.0	36.0	41.0	32.0	41.0
18	37.21042551138342	39.0	36.0	40.0	32.0	41.0
19	37.02799507104604	38.0	35.0	40.0	32.0	41.0
20	36.77130969919774	38.0	35.0	40.0	31.0	41.0
21	36.64179480805584	38.0	35.0	40.0	31.0	41.0
22	36.544440852330155	38.0	35.0	40.0	31.0	41.0
23	36.418134850737275	38.0	35.0	40.0	31.0	41.0
24	36.28174325948648	38.0	35.0	40.0	30.0	41.0
25	36.17290738467052	38.0	35.0	40.0	30.0	41.0
26	35.93800074974886	38.0	35.0	40.0	30.0	41.0
27	35.92271227653726	38.0	35.0	40.0	30.0	41.0
28	35.881366695230646	38.0	35.0	40.0	30.0	41.0
29	35.8252742742311	38.0	35.0	40.0	30.0	41.0
30	35.739102071236815	38.0	35.0	40.0	29.0	41.0
31	35.582024793296554	38.0	34.0	40.0	28.0	41.0
32	35.25780154601239	38.0	34.0	40.0	26.0	41.0
33	34.86891801811937	38.0	34.0	40.0	24.0	41.0
34	34.40214643404589	38.0	33.0	40.0	22.0	41.0
35	34.07052797706106	37.0	33.0	40.0	20.0	41.0
36	33.78081896944662	37.0	33.0	40.0	18.0	41.0
37	33.71348787528021	37.0	33.0	40.0	18.0	41.0
38	33.597151399019815	37.0	33.0	40.0	18.0	41.0
39	33.447811742774775	37.0	33.0	40.0	17.0	41.0
40	33.3233102968623	37.0	32.0	40.0	16.0	41.0
41	33.2630542349471	37.0	32.0	40.0	16.0	41.0
42	33.16547126340687	36.0	32.0	40.0	15.0	41.0
43	33.04512367521021	36.0	32.0	40.0	15.0	41.0
44	32.92479031712242	36.0	32.0	40.0	15.0	41.0
45	32.763093590091934	36.0	31.0	40.0	15.0	41.0
46	32.66774830317069	35.0	31.0	40.0	15.0	41.0
47	32.61712691433875	35.0	31.0	40.0	15.0	41.0
48	32.47003294714893	35.0	31.0	40.0	14.0	41.0
49	32.3267366402624	35.0	31.0	39.0	13.0	41.0
50	32.215576366101565	35.0	31.0	39.0	13.0	41.0
51	31.751722399036264	35.0	30.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	2.0
12	11.0
13	18.0
14	43.0
15	99.0
16	303.0
17	686.0
18	1688.0
19	3183.0
20	5375.0
21	8573.0
22	12933.0
23	19238.0
24	28297.0
25	42808.0
26	59096.0
27	66098.0
28	64238.0
29	63170.0
30	67061.0
31	76721.0
32	90725.0
33	111741.0
34	161035.0
35	200153.0
36	195279.0
37	227255.0
38	309681.0
39	433107.0
40	133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.05427763742839	22.18104878570479	25.132818055162126	21.631855521704697
2	38.48532942479677	23.106050331005672	25.74155543094328	12.667064813254278
3	26.802632392263625	23.89937890021714	36.621807730773455	12.67618097674578
4	23.69442086347411	26.49108194630535	36.43668290826071	13.377814281959823
5	21.51401243266824	29.62615280557713	35.18047557913208	13.679359182622546
6	20.410556428384975	38.040193831870376	32.230618480553446	9.318631259191205
7	82.33821144429824	2.6526256996655477	13.773277901130093	1.235884954906119
8	82.59199654208355	2.3373843192204746	13.689053444286678	1.3815656944093015
9	78.0030087786431	4.495869488556547	15.058523546160918	2.4425981866394397
10	35.52497762093035	29.654790899667503	22.252154860938482	12.568076618463655
11	28.257038456424517	25.53619717238843	30.55368908901956	15.653075282167494
12	25.20641439944716	22.048486427811326	34.85167112617526	17.893428046566253
13	22.117057764903482	24.592740954653536	35.68004133846625	17.61015994197673
14	20.291690550273863	25.52023276900576	36.14412076381888	18.0439559169015
15	19.514860013527496	24.41619866654986	38.60343932837444	17.465501991548205
16	22.730153111524476	23.460468979919096	36.63279159605345	17.176586312502973
17	22.47912509733172	24.216665858811528	33.68947145373458	19.614737590122168
18	22.993054372801282	23.81173032342814	34.906412576214464	18.288802727556117
19	24.195987731867394	26.099442668892493	31.846005319392567	17.858564279847542
20	25.844501374761926	25.44343465022615	31.975632717332676	16.736431257679257
21	24.76692638097648	25.13335168424456	33.24682612985953	16.852895804919438
22	24.05755545406721	23.066695186176517	33.04280194401075	19.83294741574553
23	21.5990373331353	26.03807532441313	33.093229892300315	19.26965745015126
24	21.475502200552928	25.187648443381732	35.13293812170568	18.203911234359666
25	21.753078261596535	25.953761929389312	33.48553620606621	18.807623602947945
26	20.758037899226817	27.347467685423876	32.51135184700143	19.383142568347882
27	20.00566536209179	26.31369474548783	33.87023830540748	19.81040158701289
28	19.579429132501435	27.139085528735258	34.35526267224546	18.926222666517845
29	20.740694954047868	25.366547593266137	34.286291113341484	19.606466339344518
30	21.02840996765763	25.732617143812593	34.487291401056495	18.751681487473277
31	22.73113143150893	26.001655139537334	32.32596020994747	18.941253219006267
32	22.713343795427953	25.97421771088243	32.29469843953516	19.01774005415446
33	22.385606600635995	26.49108194630535	31.210097329497728	19.913214123560923
34	20.815091741956543	27.846499815675624	31.806427829112398	19.531980613255435
35	21.067053607043547	28.405209464979038	31.17332139190031	19.354415536077106
36	22.992387336448246	29.147265173187098	28.560762342507157	19.2995851478575
37	21.91405636812936	29.587197882559803	28.881918111949155	19.616827637361684
38	21.93015417878264	29.302062076181777	28.75967258298266	20.008111162052924
39	21.922594433448225	28.732857721590587	28.23831696944929	21.106230875511894
40	23.228873958144803	27.99342568970447	28.36732180012656	20.410378552024167
41	20.23837211112114	27.76320920972646	29.42742044146245	22.570998237689956
42	21.42445168500053	28.582151974894533	28.43669358084236	21.556702759262578
43	21.457581157201346	28.010635227612813	29.162028911134303	21.369754704051537
44	21.27067757108051	28.73877211058751	28.9046862861328	21.08586403219918
45	21.071945206965818	28.7403729978348	28.574814575011125	21.612867220188257
46	21.58436253336849	28.76309670292824	28.595270356504248	21.05727040719901
47	20.80175101489581	28.42908936641774	29.865841201768266	20.903318416918175
48	21.532956265094477	28.169923508717943	29.843073027584623	20.45404719860296
49	21.55385673748962	27.87914012788421	29.740082614675778	20.82692051995039
50	20.720239172554745	28.61372502893826	29.7605828652591	20.905452933247894
51	20.303563797357914	29.02101742610238	29.12445252991325	21.550966246626462
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1984.0
1	2787.0
2	3590.0
3	35580.0
4	67570.0
5	46162.5
6	24755.0
7	24772.5
8	24790.0
9	26133.0
10	27476.0
11	28494.5
12	29513.0
13	29310.5
14	29108.0
15	27785.0
16	26462.0
17	24666.0
18	22870.0
19	21956.0
20	21042.0
21	20426.5
22	19811.0
23	19789.5
24	19768.0
25	20478.0
26	23491.0
27	25794.0
28	28944.5
29	32095.0
30	36211.0
31	40327.0
32	46103.5
33	51880.0
34	57260.0
35	62640.0
36	66827.0
37	71014.0
38	77013.5
39	83013.0
40	90983.5
41	98954.0
42	108177.0
43	117400.0
44	125654.5
45	133909.0
46	139206.0
47	144503.0
48	143918.0
49	143333.0
50	143327.5
51	143322.0
52	136344.0
53	129366.0
54	119499.5
55	109633.0
56	106943.5
57	104254.0
58	98951.5
59	93649.0
60	88361.0
61	83073.0
62	75988.5
63	68904.0
64	60837.5
65	52771.0
66	45288.0
67	37805.0
68	31260.5
69	24716.0
70	21750.5
71	18785.0
72	16209.0
73	13633.0
74	12208.5
75	8766.0
76	6748.0
77	4967.0
78	3186.0
79	2551.5
80	1917.0
81	1348.5
82	780.0
83	587.5
84	395.0
85	258.5
86	122.0
87	89.0
88	56.0
89	48.5
90	41.0
91	25.5
92	10.0
93	9.5
94	9.0
95	7.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2248753.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.828572892942656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.30615037839081	22.574545641491685
2	6.5674106538282695	3.786581535035529
3	2.782659528380178	2.406603091504482
4	1.66675658575571	1.9220085490900287
5	1.1450624233838398	1.6505257769745294
6	0.8651484965297094	1.4964597897255885
7	0.6703837813943435	1.3528345395721357
8	0.5737156298641256	1.3231522284286763
9	0.4588603633816043	1.1905460490085862
>10	6.048425578938142	41.442630234729386
>50	0.8572956639459858	15.866530335210047
>100	0.05531073237843363	2.3664097764862544
>500	0.001880122552649599	0.3319374888890563
>1k	7.83384396937333E-4	0.27990428883460083
>5k	0.0	0.0
>10k+	1.566768793874666E-4	2.0093306750195343
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44486	1.9782519467455966	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.4469090202436636E-5	0.0	0.0	0.031039424961300775	0.0
2	4.4469090202436636E-5	0.0	0.0	0.08916052585588546	0.0
3	4.4469090202436636E-5	0.0	0.0	0.14919379762917492	0.0
4	4.4469090202436636E-5	0.0	0.0	0.2167868147368786	0.0
5	4.4469090202436636E-5	0.0	0.0	0.3982207027628201	0.0
6	4.4469090202436636E-5	0.0	0.0	0.8273474232163337	0.0
7	4.4469090202436636E-5	0.0	0.0	1.0933615208073098	0.0
8	4.4469090202436636E-5	0.0	0.0	1.4956733798687538	0.0
9	4.4469090202436636E-5	0.0	0.0	1.7521710921564084	0.0
10	4.4469090202436636E-5	0.0	0.0	2.058785469102209	0.0
11	4.4469090202436636E-5	0.0	0.0	2.2914477490413576	0.0
12	4.4469090202436636E-5	0.0	0.0	2.480485851491916	0.0
13	4.4469090202436636E-5	0.0	0.0	2.6170948965938012	0.0
14	4.4469090202436636E-5	0.0	0.0	2.7116806514543836	0.0
15	4.4469090202436636E-5	0.0	0.0	2.7808300867191726	0.0
16	4.4469090202436636E-5	0.0	0.0	2.8674558744335195	0.0
17	4.4469090202436636E-5	0.0	0.0	2.964176145623819	0.0
18	4.4469090202436636E-5	0.0	0.0	3.0894455727240833	0.0
19	4.4469090202436636E-5	0.0	0.0	3.172736178673247	0.0
20	4.4469090202436636E-5	0.0	0.0	3.2639422826784443	0.0
21	4.4469090202436636E-5	0.0	0.0	3.3732028373058314	0.0
22	4.4469090202436636E-5	0.0	0.0	3.490289951808847	0.0
23	4.4469090202436636E-5	0.0	0.0	3.6268989969107324	0.0
24	4.4469090202436636E-5	0.0	0.0	3.7411845587309944	0.0
25	4.4469090202436636E-5	0.0	0.0	3.8502227679073693	0.0
26	4.4469090202436636E-5	0.0	0.0	3.956326017130383	0.0
27	4.4469090202436636E-5	0.0	0.0	4.068521531711131	4.4469090202436636E-5
28	4.4469090202436636E-5	0.0	0.0	4.184852671680705	4.4469090202436636E-5
29	4.4469090202436636E-5	0.0	0.0	4.306431164294167	4.4469090202436636E-5
30	4.4469090202436636E-5	0.0	0.0	4.465941790850307	4.4469090202436636E-5
31	4.4469090202436636E-5	0.0	0.0	4.599793752359641	4.4469090202436636E-5
32	4.4469090202436636E-5	0.0	0.0	4.760949735253272	4.4469090202436636E-5
33	4.4469090202436636E-5	0.0	0.0	4.907341980199693	4.4469090202436636E-5
34	4.4469090202436636E-5	0.0	0.0	5.047908774329595	4.4469090202436636E-5
35	4.4469090202436636E-5	0.0	0.0	5.197147041048972	4.4469090202436636E-5
36	4.4469090202436636E-5	0.0	0.0	5.335579318849158	4.4469090202436636E-5
37	4.4469090202436636E-5	0.0	0.0	5.48855298914554	4.4469090202436636E-5
38	4.4469090202436636E-5	0.0	0.0	5.6721213935011985	4.4469090202436636E-5
39	4.4469090202436636E-5	0.0	0.0	5.9276407858044	4.4469090202436636E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	25595	0.0	43.39129	1
CGTTAGG	240	0.0	43.124996	2
TACGGGA	605	0.0	40.16529	4
GTTTACG	40	3.4601908E-7	39.375004	1
GGGCGAT	4490	0.0	39.237194	7
AGGGCGA	2305	0.0	38.167027	6
GTTTTTT	29740	0.0	38.092636	2
GGCGATA	910	0.0	38.076927	8
GCGTTAG	155	0.0	37.741936	1
CGTAAGG	430	0.0	37.67442	2
CGCAATA	30	1.14026945E-4	37.499996	43
CGCGTAA	30	1.14026945E-4	37.499996	31
AACACGT	1070	0.0	37.429905	41
CACGACC	1165	0.0	37.274677	27
GACCGAT	1195	0.0	37.092052	9
ACGACCA	1180	0.0	36.991528	28
AGGGATC	1640	0.0	36.905487	6
TATGGGA	2015	0.0	36.848637	4
GGCGATT	1240	0.0	36.834675	8
TTACGCG	55	2.752131E-9	36.818184	1
>>END_MODULE