FastQCFastQC Report
Thu 26 May 2016
SRR1547298_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547298_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences892574
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78750.8822797885665503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAT302.164954E-644.99999612
ACGGGTA700.041.7857135
CGTTTTT44800.041.6350441
AGGGCGA12500.039.0599986
GGGCGAT24450.038.926387
TCTAGCG700.038.5714261
GCGATAC700.038.5714269
CGGTAGT1400.038.57142612
GGCACCG4450.037.921358
GGCGATT5000.037.88
ACGGGAC2750.037.6363645
ATACGAT301.1397176E-437.49999610
TCGACGG601.546141E-1037.4999962
ATACCGG301.1397176E-437.4999962
TAGGGCG5250.037.2857175
GTAGGGC3450.037.1739124
CGGGTAC850.037.0588236
TTAGGGA12400.037.016134
GCGTAAG552.748493E-936.818181
GGCGATA4650.036.7741938
Produced by FastQC (version 0.11.5)