##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547298_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	892574
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.256984855037228	31.0	31.0	33.0	30.0	34.0
2	31.65609238001555	31.0	31.0	34.0	30.0	34.0
3	31.733512291417853	31.0	31.0	34.0	30.0	34.0
4	35.53005016950976	37.0	35.0	37.0	33.0	37.0
5	35.43478747980559	37.0	35.0	37.0	33.0	37.0
6	35.465414632288194	37.0	35.0	37.0	33.0	37.0
7	35.870570955461396	37.0	35.0	37.0	35.0	37.0
8	35.85742582687822	37.0	35.0	37.0	35.0	37.0
9	37.56738825016189	39.0	37.0	39.0	35.0	39.0
10	36.86276544017639	39.0	37.0	39.0	32.0	39.0
11	36.62037769417438	39.0	35.0	39.0	32.0	39.0
12	36.331450389547534	38.0	35.0	39.0	32.0	39.0
13	36.25119821997952	39.0	35.0	39.0	32.0	39.0
14	37.316339037435554	39.0	36.0	41.0	32.0	41.0
15	37.40796057245674	39.0	36.0	41.0	32.0	41.0
16	37.45090827203123	39.0	36.0	41.0	32.0	41.0
17	37.33974886115885	39.0	36.0	41.0	32.0	41.0
18	37.187259543746514	39.0	36.0	41.0	32.0	41.0
19	37.12341273664705	39.0	35.0	41.0	31.0	41.0
20	36.98697026801139	39.0	35.0	41.0	31.0	41.0
21	36.86410314438915	39.0	35.0	41.0	31.0	41.0
22	36.74780466381499	38.0	35.0	41.0	31.0	41.0
23	36.60088575288996	38.0	35.0	40.0	30.0	41.0
24	36.492847651847356	38.0	35.0	40.0	30.0	41.0
25	36.36845012290298	38.0	35.0	40.0	30.0	41.0
26	36.14158041798215	38.0	35.0	40.0	30.0	41.0
27	36.166045616385865	38.0	35.0	40.0	30.0	41.0
28	36.17739705615445	38.0	35.0	40.0	30.0	41.0
29	36.1586703175311	38.0	35.0	40.0	30.0	41.0
30	36.12194171015513	38.0	35.0	40.0	30.0	41.0
31	36.03667258961162	38.0	35.0	40.0	30.0	41.0
32	35.864867226694926	38.0	35.0	40.0	29.0	41.0
33	35.6695254399075	38.0	34.0	40.0	27.0	41.0
34	35.41818157374067	38.0	34.0	40.0	26.0	41.0
35	35.252690533221894	38.0	34.0	40.0	25.0	41.0
36	35.0291281171085	38.0	34.0	40.0	24.0	41.0
37	34.99920566810147	38.0	34.0	40.0	24.0	41.0
38	34.90122723718145	38.0	34.0	40.0	24.0	41.0
39	34.753524077555475	38.0	33.0	40.0	24.0	41.0
40	34.632421513510366	38.0	33.0	40.0	23.0	41.0
41	34.59519434803165	38.0	33.0	40.0	23.0	41.0
42	34.51098396323442	37.0	33.0	40.0	23.0	41.0
43	34.384882373898414	37.0	33.0	40.0	23.0	41.0
44	34.29393305204947	37.0	33.0	40.0	23.0	41.0
45	34.13461853022831	37.0	33.0	40.0	23.0	41.0
46	34.025146374418256	37.0	33.0	40.0	23.0	41.0
47	33.97599526761927	37.0	33.0	40.0	23.0	41.0
48	33.835202459403924	36.0	33.0	40.0	22.0	41.0
49	33.689036427231805	36.0	32.0	40.0	22.0	41.0
50	33.56825876621994	36.0	32.0	40.0	21.0	41.0
51	33.078077559955815	35.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	11.0
15	38.0
16	91.0
17	267.0
18	526.0
19	1057.0
20	1719.0
21	2766.0
22	4016.0
23	5805.0
24	8135.0
25	11693.0
26	15559.0
27	18654.0
28	19979.0
29	22119.0
30	25167.0
31	29670.0
32	35862.0
33	44516.0
34	64590.0
35	79159.0
36	77528.0
37	95060.0
38	132830.0
39	195703.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.51747418141241	22.783209011241645	27.200433801567154	15.498883005778794
2	34.50212531397957	24.527938299793632	27.720502725824414	13.249433660402389
3	29.893879947208855	25.484497643892833	31.2836806808175	13.33794172808081
4	25.81522652463549	28.56614689650382	31.0877305411092	14.530896037751493
5	23.410047794356547	32.18343801186232	29.607965277948946	14.798548915832189
6	22.60585676929868	41.115246466959604	26.80763723792089	9.471259525820829
7	88.30786018862301	2.8496236726590736	7.589062643545522	1.253453495172389
8	88.22697053689666	2.5957511646093208	7.707148090802556	1.470130207691463
9	82.55808481985807	5.454785821679771	9.309816328954238	2.6773130295079177
10	36.00777078427111	36.265564535825604	15.644529193097714	12.082135486805576
11	30.042999235917694	26.3524368847849	27.125706103919672	16.47885777537773
12	27.184636792019486	23.55210884475685	30.915083791371924	18.348170571851746
13	24.379827330843156	25.47990418721585	31.8631284352894	18.277140046651592
14	21.68817375366076	27.01792792530367	31.834335304411738	19.459563016623832
15	21.680443302179988	25.50343164824429	34.58615196050972	18.229973089066004
16	25.06335609148373	24.673024309469017	32.677738764517	17.585880834530247
17	24.870767017636634	24.3772505136829	29.93073963615342	20.82124283252705
18	24.699688765301254	24.733523494970726	31.210409445043215	19.356378294684813
19	26.444306018324532	25.943283133947435	28.59942144852976	19.012989399198275
20	28.172902190742725	25.934432327179596	28.10601698010473	17.786648501972945
21	27.453522060915958	25.30087141234228	29.483045663440787	17.76256086330097
22	26.323083576263706	23.690472722709828	29.171138751520882	20.815304949505588
23	23.413408860217753	26.545025958632003	29.867887704548867	20.17367747660138
24	23.144635626849986	25.893091217086763	31.56197693412535	19.4002962219379
25	23.583702863852185	26.806740953691232	29.54981883855008	20.0597373439065
26	22.78197662042587	28.22533481817754	28.780358827391346	20.212329734005248
27	22.31445235913213	27.02341766621031	29.569985233717315	21.092144740940245
28	21.25594068390968	27.63143448050246	31.051655100865588	20.060969734722274
29	22.34571027164134	25.830127249953506	31.01165841711724	20.812504061287914
30	23.246139815858406	26.462903916089868	30.237604949281515	20.053351318770208
31	24.197321454579676	26.870713240582855	28.723556814336966	20.208408490500506
32	24.79828003056329	26.831052663420625	28.322245550508978	20.048421755507107
33	24.393383629816686	26.962918480708602	28.126855588444204	20.5168423010305
34	22.858945028647483	27.591213725696694	29.48091698839536	20.068924257260463
35	22.88516134236489	27.977288157620546	29.482597521325964	19.654952978688602
36	24.410300994651422	28.556175734448907	27.285020625740835	19.748502645158833
37	23.167490874706186	29.08879263792134	27.720502725824414	20.02321376154806
38	22.851774754810243	28.769043238991948	27.86648501972946	20.51269698646835
39	22.942523533062804	28.196877793886	27.811811681720506	21.04878699133069
40	24.37444962546523	27.12223300252976	28.20359992560841	20.2997174463966
41	21.15846977393471	27.563989092220925	29.0783733337516	22.199167800092763
42	22.779287767736907	27.538781098261882	27.42058361547614	22.261347518525074
43	22.36184338777513	27.14990577812036	28.46038535740454	22.027865476699972
44	22.03033025833152	28.069045255631465	28.09604581804982	21.804578667987194
45	22.208242677918022	27.87466361332506	27.513349033245422	22.403744675511497
46	22.589275511050065	28.086970940224564	28.039803982638972	21.283949566086395
47	22.129593736765806	27.806321940813874	28.91580978159794	21.148274540822385
48	22.563283268390073	27.51234071348706	29.34479382101652	20.579582197106348
49	22.689435273714	27.276842032145233	28.930486435858537	21.103236258282227
50	21.57031237746114	28.697340500619557	28.797724334340906	20.934622787578398
51	21.59428798060441	28.200574966333324	28.37568649770215	21.829450555360115
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	580.0
1	936.5
2	1293.0
3	7012.0
4	12731.0
5	8824.5
6	4918.0
7	4975.0
8	5032.0
9	5292.5
10	5553.0
11	5694.5
12	5836.0
13	5863.0
14	5890.0
15	5632.5
16	5375.0
17	5108.5
18	4842.0
19	4852.0
20	4862.0
21	5027.0
22	5192.0
23	5661.0
24	6130.0
25	6600.0
26	8755.0
27	10440.0
28	11927.0
29	13414.0
30	15403.5
31	17393.0
32	19764.5
33	22136.0
34	25848.5
35	29561.0
36	32038.0
37	34515.0
38	36263.5
39	38012.0
40	41626.0
41	45240.0
42	49591.0
43	53942.0
44	55252.5
45	56563.0
46	57989.5
47	59416.0
48	59820.0
49	60224.0
50	58080.5
51	55937.0
52	54712.0
53	53487.0
54	50373.0
55	47259.0
56	45601.0
57	43943.0
58	41486.0
59	39029.0
60	37167.0
61	35305.0
62	31831.5
63	28358.0
64	25098.5
65	21839.0
66	19180.0
67	16521.0
68	13908.5
69	11296.0
70	9766.0
71	8236.0
72	7060.0
73	5884.0
74	4976.5
75	3348.0
76	2627.0
77	1993.5
78	1360.0
79	1021.5
80	683.0
81	510.0
82	337.0
83	236.5
84	136.0
85	93.5
86	51.0
87	40.0
88	29.0
89	24.0
90	19.0
91	12.5
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	892574.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.808173726204636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.49570096866836	21.31156275150844
2	5.900766078466287	3.517821207692366
3	3.13186356838931	2.800653999999592
4	2.3041193702752714	2.747263619003141
5	1.868876683960183	2.785390043416918
6	1.5534523915092404	2.77833472568973
7	1.363132504748845	2.844274335937303
8	1.2305775269194335	2.9345014964782186
9	1.1360999894493429	3.0478559270260743
>10	9.93516458502227	52.51099489712775
>50	0.0685122029669447	1.289985720995792
>100	0.01135560931401299	0.54282509022743
>500	0.0	0.0
>1k	0.0	0.0
>5k	3.785203104670112E-4	0.8885361848971849
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7875	0.8822797885665503	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.015797009547667756	0.0
2	0.0	0.0	0.0	0.05176041426257095	0.0
3	0.0	0.0	0.0	0.08290629124307901	0.0
4	0.0	0.0	0.0	0.13533891867789113	0.0
5	0.0	0.0	0.0	0.23538664581312024	0.0
6	0.0	0.0	0.0	0.48970729597770046	0.0
7	0.0	0.0	0.0	0.6120500933255955	0.0
8	0.0	0.0	0.0	0.8884417426454277	0.0
9	0.0	0.0	0.0	1.0229964126223707	0.0
10	0.0	0.0	0.0	1.1979959084624916	0.0
11	0.0	0.0	0.0	1.3457707708268447	0.0
12	0.0	0.0	0.0	1.464416395727413	0.0
13	0.0	0.0	0.0	1.5380237380878223	0.0
14	0.0	0.0	0.0	1.5848545890872914	0.0
15	0.0	0.0	0.0	1.626979947881072	0.0
16	0.0	0.0	0.0	1.6912883413588118	0.0
17	0.0	0.0	0.0	1.756829125652327	0.0
18	0.0	0.0	0.0	1.8387270971370442	0.0
19	0.0	0.0	0.0	1.8940726483182346	0.0
20	0.0	0.0	0.0	1.9552440469921821	0.0
21	0.0	0.0	0.0	2.0344531657879346	0.0
22	0.0	0.0	0.0	2.119488132076444	0.0
23	0.0	0.0	0.0	2.214718331477278	0.0
24	0.0	0.0	0.0	2.2894460291247563	0.0
25	0.0	0.0	0.0	2.3619330161980967	0.0
26	0.0	0.0	0.0	2.433747790099196	0.0
27	0.0	0.0	0.0	2.511052304906932	0.0
28	0.0	0.0	0.0	2.588468855243375	0.0
29	0.0	0.0	0.0	2.676416745278263	0.0
30	0.0	0.0	0.0	2.7755681881838368	0.0
31	0.0	0.0	0.0	2.86833360595312	0.0
32	1.1203552870686352E-4	0.0	0.0	2.9678211554448146	0.0
33	1.1203552870686352E-4	0.0	0.0	3.066188349649441	0.0
34	1.1203552870686352E-4	0.0	0.0	3.1707174979329444	0.0
35	1.1203552870686352E-4	0.0	0.0	3.281184529237912	0.0
36	1.1203552870686352E-4	0.0	0.0	3.3899710276122765	0.0
37	1.1203552870686352E-4	0.0	0.0	3.4958446022402625	0.0
38	1.1203552870686352E-4	0.0	0.0	3.6254697089541037	0.0
39	1.1203552870686352E-4	0.0	0.0	3.810328331320428	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAAT	30	2.164954E-6	44.999996	12
ACGGGTA	70	0.0	41.785713	5
CGTTTTT	4480	0.0	41.635044	1
AGGGCGA	1250	0.0	39.059998	6
GGGCGAT	2445	0.0	38.92638	7
TCTAGCG	70	0.0	38.571426	1
GCGATAC	70	0.0	38.571426	9
CGGTAGT	140	0.0	38.571426	12
GGCACCG	445	0.0	37.92135	8
GGCGATT	500	0.0	37.8	8
ACGGGAC	275	0.0	37.636364	5
ATACGAT	30	1.1397176E-4	37.499996	10
TCGACGG	60	1.546141E-10	37.499996	2
ATACCGG	30	1.1397176E-4	37.499996	2
TAGGGCG	525	0.0	37.285717	5
GTAGGGC	345	0.0	37.173912	4
CGGGTAC	85	0.0	37.058823	6
TTAGGGA	1240	0.0	37.01613	4
GCGTAAG	55	2.748493E-9	36.81818	1
GGCGATA	465	0.0	36.774193	8
>>END_MODULE