##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547297_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	619015
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.275864074376226	31.0	31.0	33.0	30.0	34.0
2	31.6546238782582	31.0	31.0	34.0	30.0	34.0
3	31.567889308013537	31.0	31.0	34.0	30.0	34.0
4	35.43557587457493	37.0	35.0	37.0	33.0	37.0
5	35.369575858420234	37.0	35.0	37.0	33.0	37.0
6	35.42124665799698	37.0	35.0	37.0	33.0	37.0
7	35.92334272998231	37.0	35.0	37.0	35.0	37.0
8	35.942594282852596	37.0	35.0	37.0	35.0	37.0
9	37.689824963853866	39.0	37.0	39.0	35.0	39.0
10	36.96225454956665	39.0	37.0	39.0	32.0	39.0
11	36.59853638441718	39.0	35.0	39.0	32.0	39.0
12	36.11389223201376	38.0	35.0	39.0	32.0	39.0
13	35.83492484027043	38.0	35.0	39.0	31.0	39.0
14	36.970297973393215	39.0	35.0	41.0	31.0	41.0
15	37.20709352762049	39.0	35.0	41.0	32.0	41.0
16	37.33345718601326	39.0	35.0	41.0	32.0	41.0
17	37.2754359748956	39.0	35.0	41.0	32.0	41.0
18	37.04985178065152	39.0	35.0	40.0	32.0	41.0
19	36.8572280154762	38.0	35.0	40.0	32.0	41.0
20	36.55450514123244	38.0	35.0	40.0	31.0	41.0
21	36.40138122662617	38.0	35.0	40.0	31.0	41.0
22	36.27797710879381	37.0	35.0	40.0	31.0	41.0
23	36.21816111079699	37.0	35.0	40.0	31.0	41.0
24	36.01843412518275	37.0	35.0	40.0	31.0	41.0
25	35.864119609379415	37.0	35.0	40.0	30.0	41.0
26	35.56272465126047	36.0	34.0	40.0	30.0	41.0
27	35.4614427760232	36.0	34.0	40.0	30.0	41.0
28	35.36226747332456	36.0	34.0	40.0	29.0	41.0
29	35.313717761282035	36.0	34.0	40.0	29.0	41.0
30	35.21458284532685	36.0	34.0	40.0	29.0	41.0
31	35.029798954791076	35.0	34.0	40.0	28.0	41.0
32	34.698918443010264	35.0	34.0	40.0	25.0	41.0
33	34.364588903338365	35.0	33.0	40.0	24.0	41.0
34	34.01418705524099	35.0	33.0	40.0	23.0	41.0
35	33.73441031315881	35.0	33.0	40.0	21.0	41.0
36	33.403242247764595	35.0	33.0	40.0	20.0	41.0
37	33.2776766314225	35.0	33.0	40.0	18.0	41.0
38	33.22276035314169	35.0	33.0	40.0	18.0	41.0
39	33.08493655242603	35.0	33.0	40.0	18.0	41.0
40	32.967546828429036	35.0	33.0	40.0	18.0	41.0
41	32.95751476135473	35.0	33.0	40.0	18.0	41.0
42	32.85481288821757	35.0	33.0	40.0	18.0	41.0
43	32.728638239784175	35.0	32.0	39.0	17.0	41.0
44	32.61550204760789	35.0	32.0	39.0	16.0	41.0
45	32.477771944137054	35.0	31.0	39.0	17.0	41.0
46	32.4128769092833	35.0	31.0	39.0	15.0	41.0
47	32.38880479471418	35.0	31.0	39.0	15.0	41.0
48	32.25887902554865	35.0	31.0	39.0	15.0	41.0
49	32.12867862652763	35.0	31.0	39.0	15.0	41.0
50	31.968960364449973	35.0	31.0	39.0	14.0	40.0
51	31.58303756774876	35.0	30.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	10.0
15	25.0
16	71.0
17	190.0
18	480.0
19	862.0
20	1580.0
21	2477.0
22	3757.0
23	5680.0
24	8259.0
25	11760.0
26	15654.0
27	16658.0
28	16725.0
29	17071.0
30	18739.0
31	22018.0
32	26461.0
33	34017.0
34	52644.0
35	79926.0
36	65766.0
37	54320.0
38	66596.0
39	97232.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.030176974709818	24.011696000904664	27.393035709958564	20.565091314426954
2	36.42173452985792	24.842370540293853	26.732470134003215	12.003424795845012
3	24.979200827120508	24.14174131483082	38.27677843024805	12.60227942780062
4	21.85439771249485	27.17866287569768	37.98114746815505	12.985791943652417
5	20.366226989652915	30.526562361170566	36.66276261479931	12.444448034377196
6	19.653966382074746	37.79262214970558	33.37657407332617	9.1768373948935
7	81.74987682043246	3.2099383698294868	13.770587142476353	1.269597667261698
8	82.1928386226505	2.8199639750248378	13.379966559776419	1.607230842548242
9	78.22718350928491	4.877264686639258	14.798025895979904	2.0975259080959265
10	41.90835440175117	27.31032365936205	20.191271617004432	10.590050321882345
11	31.873217934945036	25.33670428018707	28.872482896214148	13.917594888653747
12	28.77086984968054	24.87144899558169	31.378884195051814	14.978796959685953
13	18.478873694498517	36.65840084650615	31.126386274969104	13.736339184026235
14	15.052139285800829	39.24573717922829	31.53057680347003	14.171546731500854
15	13.578507790602812	29.91866109868097	43.10945615211263	13.393374958603587
16	14.998667237465973	28.196731904719595	42.08702535479754	14.717575503016889
17	15.395265058197296	28.61465392599533	31.66385305687261	24.32622795893476
18	18.485174026477548	30.923321728875713	34.31710055491386	16.274403689732882
19	21.536473268014507	33.1410385854947	29.028698819899358	16.29378932659144
20	23.09701703512839	31.854801579929404	28.82353416314629	16.22464722179592
21	18.619096467775417	33.946834890915405	31.32977391501014	16.104294726299038
22	19.59387090781322	31.8221690912175	29.79572385160295	18.788236149366334
23	15.945170957085047	36.01705936043553	28.670710725911324	19.367058956568094
24	16.962270704264032	32.61471854478486	36.24274048286391	14.180270268087202
25	15.48670064538016	34.13923733673659	33.82874405305203	16.545317964831224
26	15.181053770910236	39.18321850035944	29.500900624379057	16.134827104351267
27	15.926754602069417	37.59682721743415	30.978732340896425	15.49768583960001
28	13.826320848444706	36.12868831934606	36.04064521861344	14.004345613595792
29	13.963797323166643	33.15800101774594	35.71690508307553	17.16129657601189
30	15.427412905987739	35.837742219493876	33.02682487500303	15.70801999951536
31	20.92146393867677	34.65667229388625	28.01628393496119	16.405579832475787
32	21.425652043973088	34.52840399667213	28.859559138308445	15.186384821046339
33	19.417946253321812	34.93841021623062	28.03389255510771	17.609750975339857
34	16.379247675742914	35.3862184276633	29.76115279920519	18.473381097388593
35	16.513654757962247	34.789463906367374	30.294580906763162	18.402300428907214
36	22.275712220220832	34.040370588757945	27.755547119213592	15.92837007180763
37	16.850641745353506	37.97807807565245	29.411080506934407	15.760199672059644
38	17.15515779100668	38.23299920034248	26.709692010694408	17.902150997956433
39	16.97164042874567	37.806353642480396	28.268943402017722	16.95306252675622
40	19.191134302076687	35.84226553476087	26.61599476587805	18.350605397284394
41	15.390257102008837	33.87543112848638	28.809641123397654	21.92467064610712
42	18.43751766920026	35.86181271859325	25.82990719126354	19.87076242094295
43	18.68581536796362	35.51981777501353	27.132298894210965	18.662067962811886
44	17.044821207886724	38.432348166037976	27.272037026566398	17.2507935995089
45	15.179922942093487	40.788995420143294	24.97710071646083	19.05398092130239
46	18.84849317060168	37.96903144511845	25.994038916665996	17.188436467613872
47	18.05513598216521	36.31107485279032	27.096597012996455	18.537192152048014
48	19.625211020734554	33.47576391525246	28.271043512677394	18.62798155133559
49	17.674692858816023	33.1494390281334	28.898330412025558	20.277537701025018
50	16.35453098874825	38.22831433810166	27.436007204995033	17.981147468155054
51	15.589121426782873	38.88080256536595	26.03167936156636	19.498396646284824
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	950.0
1	1047.5
2	1145.0
3	12983.5
4	24822.0
5	15405.0
6	5988.0
7	6940.0
8	7892.0
9	10736.0
10	13580.0
11	15961.0
12	18342.0
13	18418.0
14	18494.0
15	17072.0
16	15650.0
17	13661.0
18	11672.0
19	10272.0
20	8872.0
21	8014.0
22	7156.0
23	6832.0
24	6508.0
25	6583.5
26	7092.5
27	7526.0
28	7793.0
29	8060.0
30	9238.5
31	10417.0
32	11148.0
33	11879.0
34	13710.5
35	15542.0
36	16158.5
37	16775.0
38	19682.0
39	22589.0
40	28570.5
41	34552.0
42	39073.5
43	43595.0
44	45691.0
45	47787.0
46	47813.5
47	47840.0
48	47144.0
49	46448.0
50	43283.5
51	40119.0
52	36745.0
53	33371.0
54	29192.0
55	25013.0
56	21263.0
57	17513.0
58	15178.0
59	12843.0
60	11654.5
61	10466.0
62	8768.5
63	7071.0
64	5611.0
65	4151.0
66	3495.0
67	2839.0
68	2306.0
69	1773.0
70	1507.5
71	1242.0
72	980.0
73	718.0
74	623.5
75	432.0
76	335.0
77	234.5
78	134.0
79	111.0
80	88.0
81	58.0
82	28.0
83	24.5
84	21.0
85	14.0
86	7.0
87	6.5
88	6.0
89	5.5
90	5.0
91	3.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	619015.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.11288910632406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.15256213879859	25.97783907403289
2	8.650398013019666	5.901801362874118
3	3.9897780339267905	4.083085668905767
4	2.2520285643707916	3.0729280272262
5	1.4034035703013785	2.3937075182555096
6	0.9263626677274064	1.8960544173854121
7	0.7315148524503143	1.7467859528886607
8	0.5143594615548686	1.4037029818247828
9	0.42186546923234597	1.2951944972739347
>10	4.372934279714054	35.00509042506167
>50	0.5499724878354411	11.777869521668949
>100	0.03338948321619962	1.7475694384339868
>500	9.539852347485605E-4	0.26327442750336977
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.7699261737428026E-4	3.435096686664795
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21111	3.4104181643417366	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	845	0.1365071928790094	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	773	0.12487581076387486	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.029563096209300258	0.0
2	0.0	0.0	0.0	0.1287529381355864	0.0
3	0.0	0.0	0.0	0.24878233968482186	0.0
4	0.0	0.0	0.0	0.39417461612400345	0.0
5	0.0	0.0	0.0	0.7382696703634	0.0
6	0.0	0.0	0.0	1.4613539251875964	0.0
7	0.0	0.0	0.0	1.9041541804318152	0.0
8	0.0	0.0	0.0	2.6201303684078736	0.0
9	0.0	0.0	0.0	3.0734311769504776	0.0
10	0.0	0.0	0.0	3.558879833283523	0.0
11	0.0	0.0	0.0	4.019450255648086	0.0
12	0.0	0.0	0.0	4.3511061929032415	0.0
13	0.0	0.0	0.0	4.579210519938935	0.0
14	0.0	0.0	0.0	4.687931633320678	0.0
15	0.0	0.0	0.0	4.787121475246965	0.0
16	0.0	0.0	0.0	4.933967674450538	0.0
17	0.0	0.0	0.0	5.099553322617384	0.0
18	0.0	0.0	0.0	5.295671348836458	0.0
19	0.0	0.0	0.0	5.414893015516586	0.0
20	0.0	0.0	0.0	5.534276229170537	0.0
21	0.0	0.0	0.0	5.673852814552151	0.0
22	0.0	0.0	0.0	5.8434771370645295	0.0
23	0.0	0.0	0.0	6.0239251068229365	0.0
24	0.0	0.0	0.0	6.179171748665218	0.0
25	0.0	0.0	0.0	6.308086233774626	0.0
26	0.0	0.0	0.0	6.424723148873614	0.0
27	0.0	0.0	0.0	6.548306583846918	0.0
28	0.0	0.0	0.0	6.66914372026526	0.0
29	0.0	0.0	0.0	6.812758979992407	0.0
30	0.0	0.0	0.0	7.003545956075378	0.0
31	0.0	0.0	0.0	7.152492265938628	0.0
32	0.0	0.0	0.0	7.297238354482524	0.0
33	0.0	0.0	0.0	7.444569194607562	0.0
34	0.0	0.0	0.0	7.594646333287562	0.0
35	0.0	0.0	0.0	7.753447008553913	0.0
36	0.0	0.0	0.0	7.919840391589864	0.0
37	0.0	0.0	0.0	8.079771895672964	0.0
38	0.0	0.0	0.0	8.2490731242377	0.0
39	0.0	0.0	0.0	8.40658142371348	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGCGAC	30	2.1638716E-6	45.000004	29
TCTAGCG	30	2.1638716E-6	45.000004	1
CCCGTTG	30	2.1638716E-6	45.000004	37
CCGTAGG	30	2.1638716E-6	45.000004	2
TTCGAAG	30	2.1638716E-6	45.000004	1
AGTACGG	60	0.0	45.000004	2
TCACGAC	40	6.8066583E-9	45.0	25
AGGTACG	20	7.030762E-4	45.0	1
CGAGTTA	25	3.8887123E-5	45.0	31
GTCGACA	20	7.030762E-4	45.0	13
CGACGGT	40	6.8066583E-9	45.0	28
TCCGACC	20	7.030762E-4	45.0	18
GGCGTAT	35	1.2107193E-7	45.0	8
GTTAGCG	25	3.8887123E-5	45.0	1
TACGGGT	35	1.2107193E-7	45.0	4
CGACACC	20	7.030762E-4	45.0	15
CGTTCGA	20	7.030762E-4	45.0	14
CGTTCAT	20	7.030762E-4	45.0	32
TTGAGCG	25	3.8887123E-5	45.0	1
TAACCGG	25	3.8887123E-5	45.0	1
>>END_MODULE