FastQCFastQC Report
Thu 26 May 2016
SRR1547296_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547296_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1350354
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104900.7768333340738799No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT253.8911698E-545.016
TCCGTCA502.1827873E-1145.029
TTACGCG207.0337264E-445.01
TAAGCCG650.041.5384618
TACGGGA4200.040.7142834
CGTTTTT55750.040.3183861
TAGGGCG5450.039.220185
GGGCGAT26550.038.983057
AGGGCGA14400.038.593756
CGGTCTA1050.038.57142631
TCGATCA1000.038.2517
AGGGTAC5900.037.754246
TATTACG301.1400274E-437.5000041
TATCGAT301.1400274E-437.50000413
TAGCATA7050.037.34042430
GGTACCT7500.037.28
CATATGC6900.037.17391233
ATCGTTG3150.037.14285723
ATAGGGA13400.037.1082084
ACCTGGT7450.036.8456411
Produced by FastQC (version 0.11.5)