FastQCFastQC Report
Thu 26 May 2016
SRR1547295_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547295_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2049776
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212071.034600853946968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGAG800.045.01
CGTTTTT128400.041.3376161
GGGCGAT54450.039.132237
AGGGCGA28200.039.0159576
ATTAGCG1500.039.0000041
CATACGA7550.038.74172218
GACACGA11400.038.28947425
GCGAGAC11500.038.15217621
CGTTAGG2950.038.135592
CACGACC11600.037.82327727
AACACGT10600.037.7830241
TAGGGAT26500.037.6981125
TACGGGA7400.037.3986474
TATGGGC7950.037.358494
AGGGATT28400.037.315146
AAGGGAT29600.037.246625
ACACGAC11800.036.99152426
ACGACCA11800.036.99152428
TTACGGG5050.036.9801983
ACATACG7850.036.9745217
Produced by FastQC (version 0.11.5)