FastQCFastQC Report
Thu 26 May 2016
SRR1547294_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547294_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2311439
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT992854.295376170428898No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT48420.20947989542445203No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT40130.1736147914783821No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC31570.13658158402622783No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25390.10984499266474261No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC453.8562575E-1045.00000433
CTACGTA406.8193913E-945.028
CGTACTC253.892035E-545.037
GCGTAAT253.892035E-545.09
CCGAATA650.044.99999616
CGTTTTT362150.043.8319781
TATTGCG2450.042.24491
CGTTAGG2900.041.1206932
GTTGATC7500.041.10000216
TGCGTAG1150.041.0869561
CGTCGTA501.0822987E-940.530
TCGACGG1000.040.52
TTAATCG4600.040.10869220
CGACGGT1800.040.00000428
TAGCACG451.9297659E-840.0000041
CACTACG451.9297659E-840.0000041
ACCGTAG451.9297659E-840.0000041
GTTTTTT402400.039.7328532
CGTAAGG2800.039.3752
CTCATGC7700.039.1558423