FastQCFastQC Report
Thu 26 May 2016
SRR1547293_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547293_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences139527
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9570.6858887527145283No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTATG253.873147E-545.01
ACTATTC253.873147E-545.041
CCGATTG207.0119696E-445.011
TTAGCGG253.873147E-545.02
GGGTCGT207.0119696E-445.07
TATGGGA700.045.04
CGCATGG207.0119696E-445.02
CGCATCT253.873147E-545.042
ATCAGCA207.0119696E-445.022
CGATTAA207.0119696E-445.024
ACGGGAT207.0119696E-445.05
TAGTTAG253.873147E-545.01
TCAATTG207.0119696E-445.01
AATGCGG207.0119696E-445.02
AGCGATT207.0119696E-445.022
GCGATGA351.2016972E-745.09
GCGATAA207.0119696E-445.09
ATTGGGC207.0119696E-445.04
CATCGGC207.0119696E-445.033
TATAGCG253.873147E-545.01