##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547293_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	139527
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.277509012592546	31.0	31.0	33.0	30.0	34.0
2	31.685989091717015	31.0	31.0	34.0	30.0	34.0
3	31.800303883836104	31.0	31.0	34.0	30.0	34.0
4	35.583564471392634	37.0	35.0	37.0	33.0	37.0
5	35.46857597454256	37.0	35.0	37.0	33.0	37.0
6	35.51034566786357	37.0	35.0	37.0	33.0	37.0
7	35.860815469407356	37.0	35.0	37.0	35.0	37.0
8	35.8482945953113	37.0	35.0	37.0	35.0	37.0
9	37.576383065643206	39.0	37.0	39.0	35.0	39.0
10	36.917886860607624	39.0	37.0	39.0	32.0	39.0
11	36.59486694331563	39.0	35.0	39.0	32.0	39.0
12	36.102704136117026	38.0	35.0	39.0	32.0	39.0
13	35.83074243694769	38.0	35.0	39.0	30.0	39.0
14	36.85218631519348	39.0	35.0	41.0	31.0	41.0
15	37.05949386140317	39.0	35.0	41.0	31.0	41.0
16	37.220251277530515	39.0	35.0	41.0	32.0	41.0
17	37.11587004665763	39.0	35.0	41.0	32.0	41.0
18	37.003268184652434	39.0	35.0	41.0	32.0	41.0
19	36.92300414973446	38.0	35.0	41.0	31.0	41.0
20	36.77467443577229	38.0	35.0	40.0	31.0	41.0
21	36.6234420578096	38.0	35.0	40.0	31.0	41.0
22	36.52998344406459	38.0	35.0	40.0	31.0	41.0
23	36.432726282368286	38.0	35.0	40.0	31.0	41.0
24	36.299418750492734	38.0	35.0	40.0	30.0	41.0
25	36.172174561196044	38.0	35.0	40.0	30.0	41.0
26	35.907831459144106	38.0	34.0	40.0	30.0	41.0
27	35.86832656045067	38.0	34.0	40.0	30.0	41.0
28	35.92610749173995	38.0	35.0	40.0	30.0	41.0
29	35.95801529453081	38.0	35.0	40.0	30.0	41.0
30	35.945566091150816	38.0	35.0	40.0	30.0	41.0
31	35.83748665132914	38.0	35.0	40.0	30.0	41.0
32	35.62982075153913	38.0	34.0	40.0	29.0	41.0
33	35.48645065112846	38.0	34.0	40.0	28.0	41.0
34	35.25723336701857	38.0	34.0	40.0	27.0	41.0
35	35.13018268865524	37.0	34.0	40.0	26.0	41.0
36	34.86251406537803	37.0	34.0	40.0	25.0	41.0
37	34.81908877851598	37.0	34.0	40.0	25.0	41.0
38	34.73594358081232	37.0	33.0	40.0	25.0	41.0
39	34.59894500705956	37.0	33.0	40.0	24.0	41.0
40	34.437900908067974	37.0	33.0	40.0	23.0	41.0
41	34.4004386247823	37.0	33.0	40.0	23.0	41.0
42	34.29643724870455	36.0	33.0	40.0	23.0	41.0
43	34.18392139155862	36.0	33.0	40.0	23.0	41.0
44	34.06844553383933	36.0	33.0	40.0	23.0	41.0
45	33.887892665935624	36.0	33.0	40.0	23.0	41.0
46	33.816178947443866	35.0	33.0	40.0	23.0	41.0
47	33.76607394984483	35.0	33.0	40.0	23.0	41.0
48	33.62579285729644	35.0	33.0	40.0	23.0	41.0
49	33.51549879234843	35.0	32.0	40.0	22.0	41.0
50	33.364624767966056	35.0	32.0	39.0	22.0	41.0
51	32.86545256473657	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	1.0
14	1.0
15	4.0
16	23.0
17	61.0
18	82.0
19	157.0
20	294.0
21	441.0
22	642.0
23	981.0
24	1322.0
25	1755.0
26	2284.0
27	2786.0
28	3047.0
29	3496.0
30	4014.0
31	4856.0
32	6034.0
33	7493.0
34	11538.0
35	15529.0
36	11585.0
37	14226.0
38	19647.0
39	27219.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.32616626172712	23.361069900449376	28.278397729471717	15.034366108351788
2	30.825574978319608	26.145477219462897	29.404344678807686	13.624603123409806
3	28.62170045940929	26.31175328072703	31.315085969023915	13.751460290839765
4	25.57282819812653	28.567230715202076	31.157410393687247	14.702530692984153
5	22.93247901839787	32.603725443820906	29.705361686268606	14.758433851512612
6	22.876575859869416	41.26154794412551	26.126126126126124	9.735750069878948
7	88.3793100976872	3.5527173951994957	6.659642936492578	1.4083295706207402
8	88.51906799400832	2.9965526385574117	6.579371734502999	1.9050076329312609
9	83.16813233281015	5.52366208690791	8.603352756097387	2.704852824184566
10	44.64512244941839	28.906949909336543	14.552738896414313	11.89518874483075
11	35.600994789538944	25.016663441484443	23.723007016563102	15.659334752413512
12	31.905652669375822	22.21003820049166	27.65199567108875	18.23231345904377
13	24.13583034108094	29.167114608642052	28.757158112766707	17.9398969375103
14	20.0620668401098	31.43620947916891	30.256509492786343	18.245214187934952
15	18.401456348950383	25.49829065342192	38.1503221598687	17.949930837759002
16	22.432217420284246	22.4644692425122	36.48541142574555	18.617901911458
17	22.543307030180536	22.8658252524601	28.480509148766902	26.110358568592456
18	23.636285450127932	24.156614848738954	31.75586087280598	20.451238828327135
19	27.540189354031835	24.896973345660697	27.33019415596981	20.232643144337654
20	28.414572089989754	25.78067327470669	26.628537845721617	19.176216789581947
21	25.654532814437346	25.981351279680638	29.51471758154336	18.849398324338658
22	25.466755538354587	23.541680104925927	28.069835945730937	22.921728410988553
23	22.589176288460298	27.143133587047668	27.447734130311694	22.819955994180336
24	21.755645860657793	26.060189067348972	32.073362144961195	20.110802927032044
25	22.83357343023214	25.007346248396367	31.41972521447462	20.739355106896873
26	21.825883162398675	28.4095551398654	27.526571917980032	22.23798977975589
27	20.889146903466713	29.400761143004576	28.573681079647667	21.13641087388104
28	20.102202441104588	27.221971374716002	32.296258071914394	20.37956811226501
29	21.036071871394064	25.10123488643775	32.07766238792492	21.785030854243264
30	21.709059895217415	26.624954309918508	30.89437886573925	20.77160692912483
31	26.351172174561192	24.913457610354985	27.43053315845679	21.304837056627033
32	26.869351451690353	25.711152680126425	27.361012563876525	20.058483304306694
33	26.161961484157185	25.846610333483845	26.835666215141156	21.155761967217813
34	22.5554910519111	26.449361055566307	28.267647122062396	22.7275007704602
35	22.069563597009896	26.481612877794262	29.65590889218574	21.7929146330101
36	27.999598643990055	25.41300250130799	26.81488170748314	19.772517147218817
37	23.371103800698073	29.03237366244526	26.94030546059186	20.65621707626481
38	23.43775756663585	30.00566198656891	25.207307546209694	21.34927290058555
39	22.56194141635669	28.830978950310694	26.693758197338152	21.91332143599447
40	25.577128441090252	25.939065557203982	26.592702487690556	21.89110351401521
41	21.437427881341964	25.581428684053982	28.37730331763745	24.60384011696661
42	23.268614676729236	26.222881592809994	27.032043977151375	23.476459753309395
43	22.40569925534126	25.654532814437346	27.735850408881436	24.203917521339953
44	21.709059895217415	27.27859124040508	28.411705261347265	22.60064360303024
45	20.462706142897076	29.565603789947463	26.8270657292137	23.14462433794176
46	22.57985909537222	28.093487282031436	27.382513778695163	21.94413984390118
47	22.196420764439857	26.81918195044687	28.3486350312126	22.63576225390068
48	23.136740559174925	25.538426254416706	29.810000931719312	21.514832254689058
49	23.014183634708694	25.03816465630308	29.504683681294658	22.442968027693563
50	21.051839428927735	28.872547965626726	28.243279078601276	21.832333526844266
51	21.14071111684477	30.06228185225799	26.279501458499073	22.517505572398175
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	189.0
1	255.5
2	322.0
3	916.5
4	1511.0
5	1051.0
6	591.0
7	625.0
8	659.0
9	754.0
10	849.0
11	888.0
12	927.0
13	935.5
14	944.0
15	857.0
16	770.0
17	789.5
18	809.0
19	763.0
20	717.0
21	714.0
22	711.0
23	725.0
24	739.0
25	885.0
26	1127.0
27	1223.0
28	1479.5
29	1736.0
30	2100.0
31	2464.0
32	2711.5
33	2959.0
34	3214.0
35	3469.0
36	3793.0
37	4117.0
38	4753.0
39	5389.0
40	6542.5
41	7696.0
42	8651.0
43	9606.0
44	9510.0
45	9414.0
46	9413.5
47	9413.0
48	9583.5
49	9754.0
50	9633.0
51	9512.0
52	9102.0
53	8692.0
54	8331.5
55	7971.0
56	7295.5
57	6620.0
58	6358.5
59	6097.0
60	5627.0
61	5157.0
62	4811.0
63	4465.0
64	3959.5
65	3454.0
66	3088.5
67	2723.0
68	2529.5
69	2336.0
70	2029.5
71	1723.0
72	1402.5
73	1082.0
74	931.5
75	599.0
76	417.0
77	333.5
78	250.0
79	197.0
80	144.0
81	101.0
82	58.0
83	41.0
84	24.0
85	16.0
86	8.0
87	6.0
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	139527.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.58588660259304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.2134412398385	26.607036630902982
2	7.971683594952293	6.311323256430655
3	5.158148208498543	6.125696101829754
4	3.8799268553219997	6.143613780845284
5	3.1231329096735645	6.18159926035821
6	2.5799793601651184	6.127846223311617
7	2.16356163887531	5.995255398596687
8	1.739901870258722	5.5100446508561065
9	1.3307261962956929	4.741017867509514
>10	4.823203519635	25.179355966945465
>50	0.012673582821863741	0.302450421782164
>100	0.0018105118316948201	0.08887168791703398
>500	0.0018105118316948201	0.6858887527145283
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	957	0.6858887527145283	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04228572247665326	0.0
2	0.0	0.0	0.0	0.12757387459058103	0.0
3	0.0	0.0	0.0	0.1834770331190379	0.0
4	0.0	0.0	0.0	0.30961749338837646	0.0
5	0.0	0.0	0.0	0.5095787912017029	0.0
6	0.0	0.0	0.0	0.8887168791703398	0.0
7	0.0	0.0	0.0	1.0442423330251493	0.0
8	0.0	0.0	0.0	1.4154966422269526	0.0
9	0.0	0.0	0.0	1.5767557533667318	0.0
10	0.0	0.0	0.0	1.831903502547894	0.0
11	0.0	0.0	0.0	2.0748672299984947	0.0
12	0.0	0.0	0.0	2.2726784063299577	0.0
13	0.0	0.0	0.0	2.371583994495689	0.0
14	0.0	0.0	0.0	2.410286181169236	0.0
15	0.0	0.0	0.0	2.4540053179671317	0.0
16	0.0	0.0	0.0	2.5471772488478934	0.0
17	0.0	0.0	0.0	2.6482329584954885	0.0
18	0.0	0.0	0.0	2.775090125925448	0.0
19	0.0	0.0	0.0	2.848194256308815	0.0
20	0.0	0.0	0.0	2.929898872619636	0.0
21	0.0	0.0	0.0	3.016620439054807	0.0
22	0.0	0.0	0.0	3.1169594415417805	0.0
23	0.0	0.0	0.0	3.2273323442774515	0.0
24	0.0	0.0	0.0	3.322654396640077	0.0
25	0.0	0.0	0.0	3.406509134432762	0.0
26	0.0	0.0	0.0	3.483196800619235	0.0
27	0.0	0.0	0.0	3.5828190959455877	0.0
28	0.0	0.0	0.0	3.643739204598393	0.0
29	0.0	0.0	0.0	3.7369111354791547	0.0
30	0.0	0.0	0.0	3.8551678169816594	0.0
31	0.0	0.0	0.0	3.9469063335411785	0.0
32	0.0	0.0	0.0	4.04007826442194	0.0
33	0.0	0.0	0.0	4.123216295054004	0.0
34	0.0	0.0	0.0	4.233589197789675	0.0
35	0.0	0.0	0.0	4.3561461222559075	0.0
36	0.0	0.0	0.0	4.459351953385366	0.0
37	0.0	0.0	0.0	4.563991198836067	0.0
38	0.0	0.0	0.0	4.688698244784164	0.0
39	0.0	0.0	0.0	4.928795143592279	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTATG	25	3.873147E-5	45.0	1
ACTATTC	25	3.873147E-5	45.0	41
CCGATTG	20	7.0119696E-4	45.0	11
TTAGCGG	25	3.873147E-5	45.0	2
GGGTCGT	20	7.0119696E-4	45.0	7
TATGGGA	70	0.0	45.0	4
CGCATGG	20	7.0119696E-4	45.0	2
CGCATCT	25	3.873147E-5	45.0	42
ATCAGCA	20	7.0119696E-4	45.0	22
CGATTAA	20	7.0119696E-4	45.0	24
ACGGGAT	20	7.0119696E-4	45.0	5
TAGTTAG	25	3.873147E-5	45.0	1
TCAATTG	20	7.0119696E-4	45.0	1
AATGCGG	20	7.0119696E-4	45.0	2
AGCGATT	20	7.0119696E-4	45.0	22
GCGATGA	35	1.2016972E-7	45.0	9
GCGATAA	20	7.0119696E-4	45.0	9
ATTGGGC	20	7.0119696E-4	45.0	4
CATCGGC	20	7.0119696E-4	45.0	33
TATAGCG	25	3.873147E-5	45.0	1
>>END_MODULE