FastQCFastQC Report
Thu 26 May 2016
SRR1547292_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547292_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2024283
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243531.203043250375565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT134100.041.375841
TAATGCG501.0822987E-940.51
GCGTAAG1400.040.1785741
ACGTAGG3050.039.8360672
AGGGCGA21550.039.1531336
GGGCGAT42000.038.946437
GGCGATA8750.038.5714268
TAGGGCG7900.038.164565
TACGGGA5800.038.0172424
TACGAAT1600.037.96875412
CACGACC7850.037.83439327
TCAAGCG8250.037.63636417
ACGGGAT6700.037.276125
TATACGG1450.037.2413832
GTACGGG6650.036.8796963
GTATCGC552.752131E-936.8181817
TAGGGAT24050.036.7671555
TAAGGGA20500.036.6585354
GCGATAA1600.036.5625049
GTTGATC9000.036.516
Produced by FastQC (version 0.11.5)