##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547292_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2024283
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.275410108171634	31.0	31.0	33.0	30.0	34.0
2	31.67567528848486	31.0	31.0	34.0	30.0	34.0
3	31.731178397486914	31.0	31.0	34.0	30.0	34.0
4	35.56179644842149	37.0	35.0	37.0	33.0	37.0
5	35.48055385536509	37.0	35.0	37.0	33.0	37.0
6	35.507832649881465	37.0	35.0	37.0	33.0	37.0
7	35.872872024316756	37.0	35.0	37.0	35.0	37.0
8	35.86062818291711	37.0	35.0	37.0	35.0	37.0
9	37.599926986493486	39.0	37.0	39.0	35.0	39.0
10	36.92317131547318	39.0	37.0	39.0	32.0	39.0
11	36.63486380115824	39.0	35.0	39.0	32.0	39.0
12	36.295574284820844	38.0	35.0	39.0	32.0	39.0
13	36.17966559023615	38.0	35.0	39.0	31.0	39.0
14	37.2596015478073	39.0	36.0	41.0	32.0	41.0
15	37.357184741461545	39.0	36.0	41.0	32.0	41.0
16	37.41926054805578	39.0	36.0	41.0	32.0	41.0
17	37.304466816151695	39.0	35.0	41.0	32.0	41.0
18	37.12544145260322	39.0	35.0	41.0	31.0	41.0
19	37.02231901369522	39.0	35.0	41.0	31.0	41.0
20	36.8480573121446	38.0	35.0	41.0	31.0	41.0
21	36.7136635539596	38.0	35.0	40.0	31.0	41.0
22	36.616972528050674	38.0	35.0	40.0	31.0	41.0
23	36.49723778740423	38.0	35.0	40.0	30.0	41.0
24	36.34390003769236	38.0	35.0	40.0	30.0	41.0
25	36.22659381124082	38.0	35.0	40.0	30.0	41.0
26	35.97764739416376	38.0	35.0	40.0	30.0	41.0
27	35.982018818515	38.0	35.0	40.0	30.0	41.0
28	35.98221444333623	38.0	35.0	40.0	30.0	41.0
29	35.95589549484929	38.0	35.0	40.0	30.0	41.0
30	35.88817769056995	38.0	35.0	40.0	29.0	41.0
31	35.7829162226823	38.0	34.0	40.0	29.0	41.0
32	35.586483214056535	38.0	34.0	40.0	28.0	41.0
33	35.33802091901182	38.0	34.0	40.0	26.0	41.0
34	35.019701296706046	38.0	34.0	40.0	25.0	41.0
35	34.81966651896005	38.0	34.0	40.0	24.0	41.0
36	34.57686993369998	38.0	33.0	40.0	23.0	41.0
37	34.52846958651532	37.0	33.0	40.0	23.0	41.0
38	34.41650549849008	37.0	33.0	40.0	23.0	41.0
39	34.24361070067772	37.0	33.0	40.0	23.0	41.0
40	34.1234471662312	37.0	33.0	40.0	22.0	41.0
41	34.06592852876796	37.0	33.0	40.0	22.0	41.0
42	33.97032776543596	37.0	33.0	40.0	22.0	41.0
43	33.83879378525631	36.0	33.0	40.0	21.0	41.0
44	33.7243344927562	36.0	33.0	40.0	21.0	41.0
45	33.560088189250216	36.0	32.0	40.0	20.0	41.0
46	33.46396328971789	36.0	32.0	40.0	20.0	41.0
47	33.38870256777338	35.0	32.0	40.0	20.0	41.0
48	33.24332516747905	35.0	32.0	40.0	20.0	41.0
49	33.08805191764195	35.0	32.0	39.0	20.0	41.0
50	32.9721629831402	35.0	31.0	39.0	20.0	41.0
51	32.49313855819567	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	7.0
13	12.0
14	42.0
15	102.0
16	255.0
17	620.0
18	1450.0
19	2680.0
20	4293.0
21	6901.0
22	10100.0
23	14690.0
24	20645.0
25	29260.0
26	39659.0
27	46233.0
28	49517.0
29	53465.0
30	60117.0
31	70579.0
32	83871.0
33	104650.0
34	155104.0
35	186771.0
36	176642.0
37	216600.0
38	292581.0
39	397342.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.57350034555445	23.01466741557381	26.922520220739887	18.48931201813185
2	33.65043326451884	24.45848727672959	28.55208486165225	13.338994597099319
3	28.38679176775184	25.6806978075694	33.19175233897632	12.740758085702444
4	25.179483303470906	28.296339988035268	32.3514548114073	14.172721897086523
5	23.01081419939801	31.220980465675996	31.41625948545732	14.351945849468676
6	22.06104581227032	40.02938324335086	27.985662083809427	9.923908860569398
7	86.70511978809287	2.8373009109892244	9.103075014708912	1.3545042862089933
8	86.89629859066147	2.411372322941012	9.14407718683603	1.5482518995614745
9	82.3561725312123	4.55687272975172	10.585871639489143	2.5010830995468516
10	41.08970929459962	30.202743391116755	16.688575658640616	12.01897165564301
11	31.22656268911017	24.531599583655055	26.567233929248037	17.674603797986745
12	28.222980680072894	21.07215246089603	31.73311241560592	18.971754443425155
13	23.869883805772215	23.835204860190004	32.517587708833204	19.77732362520458
14	21.425759145336894	24.89395010480254	33.47185151483266	20.20843923502791
15	21.220797684908682	22.864046183265877	36.31023923038429	19.604916901441154
16	24.969927623756163	22.288928968923813	33.08168867692907	19.65945473039096
17	24.504380069387533	22.269168885970984	30.9038311342831	22.322619910358384
18	24.901952938398438	22.856240950499508	32.29084075694949	19.950965354152554
19	26.73094621651222	24.2650360646214	28.62035594825427	20.383661770612115
20	28.168492251330473	24.373321319202898	28.766284160860906	18.691902268605723
21	26.927262640648564	23.964732203945793	29.921211609246335	19.186793546159304
22	26.093930542320415	22.644511661659955	29.88169144334068	21.37986635267895
23	24.791197673447833	23.963200797516947	29.693130851763318	21.552470677271902
24	23.95796437553445	23.50046905496909	32.13508190307383	20.40648466642263
25	24.36190987129764	24.634352015009757	29.78126082173293	21.22247729195967
26	22.72740520964707	25.255707823461442	29.41836689830424	22.59852006858725
27	22.025823464406905	24.823950010942145	30.4194621009019	22.73076442374905
28	21.443197418542763	25.928637448420012	31.08527809599745	21.542887037039783
29	23.16667185368844	23.9735254408598	30.789815455645282	22.069987249806474
30	23.235436942364284	24.61627153910792	31.321905089357564	20.826386429170228
31	25.064578421100215	25.41843210657798	28.63255779947764	20.884431672844162
32	26.243514370273324	24.44351901389282	28.231329315120462	21.08163730071339
33	25.422334722961164	25.564953121673206	27.834151647768618	21.178560507597012
34	23.20762462560818	25.760083940832384	29.724055381584492	21.308236051974948
35	23.614879935265968	26.777036609999687	29.25475341145482	20.35333004327952
36	25.328276728105703	28.04341092623907	26.19362016081743	20.43469218483779
37	24.095889754545187	27.97415183548941	27.3073972364536	20.622561173511805
38	24.53821921144425	27.163840233801302	27.666240342876957	20.63170021187749
39	24.227146105559353	27.013120201078607	26.85039591796206	21.90933777539998
40	25.274183501022335	25.72851720831524	27.752789506210345	21.244509784452077
41	21.812957970797562	26.009456187697076	28.793651875750577	23.38393396575479
42	23.196756579984125	26.410141269773053	27.14141253964984	23.251689610592983
43	22.600594877297294	25.523209946435355	28.60973490366713	23.266460272600227
44	22.93291007235648	25.848757313083198	28.169924857344554	23.048407757215763
45	22.22238688958016	26.639753433684916	27.47506154030835	23.662798136426577
46	23.002119762898765	26.906909755207153	27.456388261917926	22.63458221997616
47	22.36614149306199	26.35980245845072	29.2848381377505	21.989217910736787
48	22.94066590491547	26.007331978779646	29.32722351568432	21.724778600620564
49	23.172698678989054	25.996414532948208	28.78080782183123	22.050078966231503
50	22.06178681538105	26.905971151266893	29.252728002952153	21.7795140303999
51	21.924503639066277	26.540804818298625	28.482430569243526	23.052260973391565
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1621.0
1	2663.0
2	3705.0
3	21319.0
4	38933.0
5	26518.5
6	14104.0
7	13876.0
8	13648.0
9	14094.0
10	14540.0
11	14674.5
12	14809.0
13	14857.5
14	14906.0
15	14263.0
16	13620.0
17	13123.0
18	12626.0
19	12184.5
20	11743.0
21	11587.5
22	11432.0
23	11990.0
24	12548.0
25	13852.5
26	16638.0
27	18119.0
28	21981.0
29	25843.0
30	29252.5
31	32662.0
32	36091.0
33	39520.0
34	46546.5
35	53573.0
36	57224.5
37	60876.0
38	66960.0
39	73044.0
40	79169.0
41	85294.0
42	92950.0
43	100606.0
44	102975.0
45	105344.0
46	109948.5
47	114553.0
48	117784.0
49	121015.0
50	123758.5
51	126502.0
52	128340.5
53	130179.0
54	125392.5
55	120606.0
56	118492.0
57	116378.0
58	111888.0
59	107398.0
60	102334.5
61	97271.0
62	89426.0
63	81581.0
64	73283.5
65	64986.0
66	56887.0
67	48788.0
68	41705.5
69	34623.0
70	29716.5
71	24810.0
72	21233.0
73	17656.0
74	15088.0
75	10420.0
76	8320.0
77	6259.0
78	4198.0
79	3404.5
80	2611.0
81	1841.0
82	1071.0
83	768.5
84	466.0
85	358.0
86	250.0
87	189.0
88	128.0
89	89.5
90	51.0
91	40.5
92	30.0
93	19.5
94	9.0
95	7.0
96	5.0
97	5.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2024283.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.886709908049333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.31362453894474	19.24081745840562
2	5.400286964047082	2.687907501889203
3	2.1778067012924867	1.6259533083261588
4	1.3513016822870068	1.3451781186134315
5	1.0301619962444248	1.2818671379416007
6	0.8512567728280395	1.27109882175802
7	0.7261331891388569	1.2649746222893816
8	0.634058408082731	1.262370213337159
9	0.5707122817093443	1.2782835896275242
>10	9.109385429773997	53.432861826844324
>50	0.7958806383386956	12.333996599042
>100	0.03779175916199085	1.5136736884784847
>500	0.0011997286129441405	0.1966600692214833
>1k	1.999547688240234E-4	0.05249908224409537
>5k	0.0	0.0
>10k+	1.999547688240234E-4	1.2118579619814736
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24353	1.203043250375565	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.940020738207059E-5	0.0	0.0	0.02262529498098833	0.0
2	4.940020738207059E-5	0.0	0.0	0.06767828411343671	0.0
3	4.940020738207059E-5	0.0	0.0	0.10868045624055529	0.0
4	4.940020738207059E-5	0.0	0.0	0.16751610323260138	0.0
5	4.940020738207059E-5	0.0	0.0	0.28271738684759	0.0
6	4.940020738207059E-5	0.0	0.0	0.5527883206053699	0.0
7	4.940020738207059E-5	0.0	0.0	0.6960489220133746	0.0
8	4.940020738207059E-5	0.0	0.0	0.9507563912753306	0.0
9	4.940020738207059E-5	0.0	0.0	1.110615462363711	0.0
10	4.940020738207059E-5	0.0	0.0	1.3217025485072986	0.0
11	4.940020738207059E-5	0.0	0.0	1.4959864801512437	0.0
12	4.940020738207059E-5	0.0	0.0	1.6366782707753806	0.0
13	4.940020738207059E-5	0.0	0.0	1.7254504434409617	0.0
14	4.940020738207059E-5	0.0	0.0	1.7835450873222767	0.0
15	4.940020738207059E-5	0.0	0.0	1.8270172698184988	0.0
16	4.940020738207059E-5	0.0	0.0	1.8842721101743185	0.0
17	4.940020738207059E-5	0.0	0.0	1.9492333828817414	0.0
18	4.940020738207059E-5	0.0	0.0	2.030595524440012	0.0
19	4.940020738207059E-5	0.0	0.0	2.085627355463638	0.0
20	4.940020738207059E-5	0.0	0.0	2.1424375939530194	0.0
21	4.940020738207059E-5	0.0	0.0	2.2094242751631072	0.0
22	4.940020738207059E-5	0.0	0.0	2.2889092088408587	0.0
23	4.940020738207059E-5	0.0	0.0	2.3691845458367236	0.0
24	4.940020738207059E-5	0.0	0.0	2.44377885898365	0.0
25	4.940020738207059E-5	0.0	0.0	2.509481134801804	0.0
26	4.940020738207059E-5	0.0	0.0	2.573948405435406	0.0
27	4.940020738207059E-5	0.0	0.0	2.6493825221078278	0.0
28	4.940020738207059E-5	0.0	0.0	2.719975418456807	0.0
29	4.940020738207059E-5	0.0	0.0	2.802029162918426	0.0
30	4.940020738207059E-5	0.0	0.0	2.9011753791342416	0.0
31	9.880041476414118E-5	0.0	0.0	2.9844147285730305	0.0
32	9.880041476414118E-5	0.0	0.0	3.0829187420928794	0.0
33	1.4820062214621177E-4	0.0	0.0	3.173172920979922	0.0
34	1.4820062214621177E-4	0.0	0.0	3.264909106088427	0.0
35	1.4820062214621177E-4	0.0	0.0	3.3617829127646677	0.0
36	1.4820062214621177E-4	0.0	0.0	3.4539636997396115	0.0
37	1.4820062214621177E-4	0.0	0.0	3.5538015188587764	0.0
38	1.4820062214621177E-4	0.0	0.0	3.670484808695227	0.0
39	1.4820062214621177E-4	0.0	0.0	3.856723590525633	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	13410	0.0	41.37584	1
TAATGCG	50	1.0822987E-9	40.5	1
GCGTAAG	140	0.0	40.178574	1
ACGTAGG	305	0.0	39.836067	2
AGGGCGA	2155	0.0	39.153133	6
GGGCGAT	4200	0.0	38.94643	7
GGCGATA	875	0.0	38.571426	8
TAGGGCG	790	0.0	38.16456	5
TACGGGA	580	0.0	38.017242	4
TACGAAT	160	0.0	37.968754	12
CACGACC	785	0.0	37.834393	27
TCAAGCG	825	0.0	37.636364	17
ACGGGAT	670	0.0	37.27612	5
TATACGG	145	0.0	37.241383	2
GTACGGG	665	0.0	36.879696	3
GTATCGC	55	2.752131E-9	36.81818	17
TAGGGAT	2405	0.0	36.767155	5
TAAGGGA	2050	0.0	36.658535	4
GCGATAA	160	0.0	36.562504	9
GTTGATC	900	0.0	36.5	16
>>END_MODULE