FastQCFastQC Report
Thu 26 May 2016
SRR1547291_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547291_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2733172
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226630.8291830883676549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT149250.041.3517571
TACGGGA10000.040.954
CGTAAGG4100.040.6097532
AGGGCGA34900.039.7779356
CGCGTAA403.4605546E-739.37531
ATAGTCG750.039.01
ACGGGAT11000.038.8636365
GCGAGAC20250.038.66666821
TCGACGC356.25145E-638.57142636
GACACGA20200.038.539625
CACGACC20500.038.19512627
ACACGAC20450.038.0684626
TCAAGCG20400.038.0514717
CACGTGA18800.037.8191543
GGGCGAT69200.037.8143047
CGTTAGG4600.037.6630442
AACGGGA11900.037.626054
AACACGT19100.037.57853341
CGACCAA20750.037.3012129
TAAGGGA35250.037.0212754
Produced by FastQC (version 0.11.5)