##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547291_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2733172
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29335951048818	31.0	31.0	33.0	30.0	34.0
2	31.702570127309954	31.0	31.0	34.0	30.0	34.0
3	31.766338525347106	31.0	31.0	34.0	30.0	34.0
4	35.56574997841336	37.0	35.0	37.0	33.0	37.0
5	35.47162271529198	37.0	35.0	37.0	33.0	37.0
6	35.50648038250063	37.0	35.0	37.0	33.0	37.0
7	35.9089808471622	37.0	35.0	37.0	35.0	37.0
8	35.90325453356027	37.0	35.0	37.0	35.0	37.0
9	37.61289446840521	39.0	37.0	39.0	35.0	39.0
10	36.86597440629423	39.0	37.0	39.0	32.0	39.0
11	36.650251795349874	39.0	35.0	39.0	32.0	39.0
12	36.38493186671018	39.0	35.0	39.0	32.0	39.0
13	36.29896106062846	39.0	35.0	39.0	32.0	39.0
14	37.408028839751026	39.0	36.0	41.0	32.0	41.0
15	37.48547621591323	39.0	36.0	41.0	32.0	41.0
16	37.52359492926168	39.0	36.0	41.0	32.0	41.0
17	37.40711378574053	39.0	36.0	41.0	32.0	41.0
18	37.26101686977621	39.0	36.0	41.0	32.0	41.0
19	37.1934459302232	39.0	36.0	41.0	32.0	41.0
20	37.04957975568314	39.0	35.0	41.0	31.0	41.0
21	36.916257008340494	39.0	35.0	41.0	31.0	41.0
22	36.78088901832742	39.0	35.0	41.0	31.0	41.0
23	36.63809705353341	38.0	35.0	40.0	31.0	41.0
24	36.53736244919822	38.0	35.0	40.0	30.0	41.0
25	36.43751728760576	38.0	35.0	40.0	30.0	41.0
26	36.224323972293	38.0	35.0	40.0	30.0	41.0
27	36.22721402092514	38.0	35.0	40.0	30.0	41.0
28	36.25412560936523	38.0	35.0	40.0	30.0	41.0
29	36.239183263987776	38.0	35.0	40.0	30.0	41.0
30	36.18371877071769	38.0	35.0	40.0	30.0	41.0
31	36.09764112906176	38.0	35.0	40.0	30.0	41.0
32	35.92262872589065	38.0	35.0	40.0	29.0	41.0
33	35.722845104515926	38.0	35.0	40.0	27.0	41.0
34	35.47844006890163	38.0	34.0	40.0	26.0	41.0
35	35.314366604077605	38.0	34.0	40.0	25.0	41.0
36	35.08506050844952	38.0	34.0	40.0	24.0	41.0
37	35.04615443155425	38.0	34.0	40.0	24.0	41.0
38	34.93640100220549	38.0	34.0	40.0	24.0	41.0
39	34.801085332353765	38.0	34.0	40.0	24.0	41.0
40	34.68546948380855	38.0	33.0	40.0	23.0	41.0
41	34.66013774471566	38.0	33.0	40.0	23.0	41.0
42	34.57386179867202	38.0	33.0	40.0	23.0	41.0
43	34.44828316695766	37.0	33.0	40.0	23.0	41.0
44	34.35779928961661	37.0	33.0	40.0	23.0	41.0
45	34.18769656648026	37.0	33.0	40.0	23.0	41.0
46	34.07704344988168	37.0	33.0	40.0	23.0	41.0
47	34.03097060850909	37.0	33.0	40.0	23.0	41.0
48	33.90514757212499	37.0	33.0	40.0	22.0	41.0
49	33.76905441735829	36.0	33.0	40.0	21.0	41.0
50	33.65862558229047	36.0	33.0	40.0	21.0	41.0
51	33.178250765045156	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	5.0
12	6.0
13	10.0
14	39.0
15	137.0
16	310.0
17	776.0
18	1750.0
19	3190.0
20	5345.0
21	8308.0
22	12368.0
23	17778.0
24	25355.0
25	35931.0
26	48005.0
27	55868.0
28	60056.0
29	65863.0
30	74996.0
31	88704.0
32	107111.0
33	132838.0
34	194503.0
35	244189.0
36	228875.0
37	284930.0
38	409612.0
39	626107.0
40	205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.196054986660194	23.47045850023343	26.541651970677293	15.79183454242909
2	34.605176695795215	24.270408155798464	27.305745851340497	13.818669297065828
3	29.085363087284666	25.611633662279576	31.4226107980032	13.880392452432558
4	26.00549837331862	28.232983507807045	30.84050326872952	14.921014850144815
5	23.006345740407117	31.960374246479915	30.12821732404693	14.905062689066037
6	22.266875264344872	40.50696406958655	27.607666111024116	9.61849455504447
7	88.2134018642076	2.9172697510438423	7.661025358082112	1.208303026666452
8	88.36637430794696	2.543967229285241	7.711369793046321	1.3782886697214811
9	83.04175514749895	5.245443755460688	9.379943889371031	2.332857207669331
10	34.27376689063111	35.77100160546062	17.812746508452452	12.14248499545583
11	29.682617852078096	27.277463694198534	27.234144064113053	15.805774389610313
12	26.80369182766397	24.534313976581057	30.68043284506061	17.98156135069436
13	23.397685912192866	27.22250923103266	31.74201989483282	17.637784961941655
14	21.783224765949598	28.74224527398934	31.55085007456538	17.92367988549568
15	21.437070188045247	26.050464442047556	34.838348995233375	17.674116374673822
16	24.1053984161992	25.42115900499493	33.73432041598553	16.73912216282034
17	23.720790349088897	24.565120672976306	30.602428240886415	21.111660737048382
18	24.147400895369923	25.107128274400587	31.175974289214142	19.569496541015347
19	26.494893113203265	26.309284596798154	28.58393105154012	18.611891238458465
20	28.6680823599832	25.91487107287796	28.135880215368807	17.28116635177003
21	26.992520046305174	25.98596063474966	29.67544669709773	17.346072621847437
22	25.502712599133897	24.19485491582674	29.58847814919807	20.713954335841287
23	22.83789677341931	27.958028254350626	29.438761995220208	19.765312977009863
24	22.11573219687601	26.93950472198603	32.465574797341695	18.47918828379626
25	22.523390404994636	27.276183130809184	30.784341417225114	19.416085046971066
26	21.204739401691512	29.094839256365862	29.32256001451793	20.377861327424693
27	21.0384490987029	28.304658470085307	30.724593988230524	19.93229844298127
28	20.441011396282416	28.865033009265424	32.119749507166034	18.57420608728613
29	21.554625907187695	26.838889027108433	31.07272429250702	20.533760773196857
30	22.13644073625809	27.191007371654617	31.427184238679455	19.245367653407836
31	23.667994549922213	27.743698530498627	29.394417914423244	19.193889005155913
32	24.161084629873276	27.128771990932147	29.296399933849752	19.41374344534482
33	23.27299562559546	27.232826913198295	28.730061628027805	20.764115833178444
34	21.394701833620424	28.42429967817613	30.293263651171603	19.887734837031847
35	21.946112429075082	28.12567229577941	29.821467511009185	20.106747764136323
36	23.870177215338074	29.409638324993814	27.59826311699373	19.121921342674373
37	22.7379762415245	29.665311952559154	27.770919649403698	19.82579215651265
38	22.773246616019772	29.61390647935805	27.79532352885219	19.817523375769987
39	22.606809962929518	28.943513251269952	27.681499737301568	20.76817704849896
40	23.37185511925338	28.128196835032703	28.32825742397478	20.171690621739135
41	20.926381508371957	27.96512623428017	28.973222321902902	22.13526993544497
42	22.559355942472703	28.26510735511706	27.818154144708053	21.357382557702188
43	21.969418682761273	27.57748140256083	28.51646365468401	21.936636259993882
44	21.57112688114762	29.326401704685985	28.161381720579605	20.941089693586793
45	21.89265805445102	28.902974273115632	27.618422843494667	21.585944828938683
46	22.401700295480858	28.89902282037135	28.053704633297865	20.645572250849927
47	21.815860838615354	28.23799599878822	29.25227537820525	20.693867784391177
48	22.344148118010867	27.999225807962326	29.17288044806547	20.483745625961337
49	21.960418151510407	28.637861063994507	29.036848028590956	20.36487275590413
50	21.380579048812148	29.206614146493525	28.753184944086946	20.659621860607384
51	21.556272345831143	28.808175994778228	28.283657230499948	21.35189442889068
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2153.0
1	3280.5
2	4408.0
3	20552.0
4	36696.0
5	26046.0
6	15396.0
7	15432.5
8	15469.0
9	16285.0
10	17101.0
11	17764.5
12	18428.0
13	18792.5
14	19157.0
15	18908.0
16	18659.0
17	17619.5
18	16580.0
19	16100.5
20	15621.0
21	16048.0
22	16475.0
23	18041.0
24	19607.0
25	22820.0
26	30434.5
27	34836.0
28	39315.0
29	43794.0
30	50700.5
31	57607.0
32	68464.5
33	79322.0
34	86003.0
35	92684.0
36	100681.0
37	108678.0
38	118182.0
39	127686.0
40	138938.0
41	150190.0
42	163395.0
43	176600.0
44	177922.5
45	179245.0
46	182458.0
47	185671.0
48	185563.0
49	185455.0
50	185098.0
51	184741.0
52	174851.5
53	164962.0
54	154316.0
55	143670.0
56	132323.0
57	120976.0
58	114550.0
59	108124.0
60	99616.5
61	91109.0
62	82443.0
63	73777.0
64	63053.0
65	52329.0
66	45503.5
67	38678.0
68	33726.5
69	28775.0
70	24775.5
71	20776.0
72	18217.5
73	15659.0
74	13507.5
75	9391.0
76	7426.0
77	5399.5
78	3373.0
79	2725.5
80	2078.0
81	1543.5
82	1009.0
83	705.5
84	402.0
85	340.5
86	279.0
87	168.5
88	58.0
89	48.5
90	39.0
91	26.0
92	13.0
93	10.0
94	7.0
95	6.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2733172.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.300029431793075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.41091651268279	17.12754888357599
2	5.600160715882464	2.3856717614213574
3	1.9953714677520107	1.2750441297143393
4	1.1374249534492655	0.9690873993970092
5	0.7776850812476458	0.8282357559620621
6	0.5866260905008618	0.7497091795875638
7	0.4669829819388229	0.6962725881610395
8	0.40324056005215486	0.6871228637762896
9	0.3412891756354382	0.6542522537208506
>10	5.84843020668146	31.485653460851122
>50	1.960135115918189	29.158772858684923
>100	0.4696537067212671	12.816693130139372
>500	0.001562573653220726	0.2340894824473861
>1k	3.472385896046058E-4	0.09374673586163093
>5k	0.0	0.0
>10k+	1.736192948023029E-4	0.8380995166990696
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22663	0.8291830883676549	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.097625762301092E-4	0.0	0.0	0.014891122841884813	0.0
2	1.097625762301092E-4	0.0	0.0	0.041526841340391314	0.0
3	1.097625762301092E-4	0.0	0.0	0.07054074899055017	0.0
4	1.097625762301092E-4	0.0	0.0	0.12560497473265494	0.0
5	1.097625762301092E-4	0.0	0.0	0.21561028724134448	0.0
6	1.097625762301092E-4	0.0	0.0	0.44044063088601815	0.0
7	1.097625762301092E-4	0.0	0.0	0.5504227322685875	0.0
8	1.097625762301092E-4	0.0	0.0	0.7644231683918904	0.0
9	1.097625762301092E-4	0.0	0.0	0.8690634910645946	0.0
10	1.097625762301092E-4	0.0	0.0	1.0219627597531367	0.0
11	1.097625762301092E-4	0.0	0.0	1.156824378414531	0.0
12	1.097625762301092E-4	0.0	0.0	1.2694041941012129	0.0
13	1.097625762301092E-4	0.0	0.0	1.3396156553630727	0.0
14	1.097625762301092E-4	0.0	0.0	1.380996146601824	0.0
15	1.097625762301092E-4	0.0	0.0	1.4179495472659607	0.0
16	1.097625762301092E-4	0.0	0.0	1.474550449075287	0.0
17	1.097625762301092E-4	0.0	0.0	1.5368224173231688	0.0
18	1.097625762301092E-4	0.0	0.0	1.615302659327697	0.0
19	1.097625762301092E-4	0.0	0.0	1.66868385890094	0.0
20	1.097625762301092E-4	0.0	0.0	1.7222114085758233	0.0
21	1.097625762301092E-4	0.0	0.0	1.7917277068548925	0.0
22	1.097625762301092E-4	0.0	0.0	1.8643539447938147	0.0
23	1.097625762301092E-4	0.0	0.0	1.947078339745907	0.0
24	1.4635010164014558E-4	0.0	0.0	2.016558050499566	0.0
25	1.4635010164014558E-4	0.0	0.0	2.08069598254336	0.0
26	1.4635010164014558E-4	0.0	0.0	2.14102881194451	0.0
27	1.4635010164014558E-4	0.0	0.0	2.2068131826317554	0.0
28	1.4635010164014558E-4	0.0	0.0	2.2762197183345942	0.0
29	1.8293762705018197E-4	0.0	0.0	2.350931445221889	0.0
30	1.8293762705018197E-4	0.0	0.0	2.4451443231527326	0.0
31	1.8293762705018197E-4	0.0	0.0	2.532222633628619	0.0
32	1.8293762705018197E-4	0.0	0.0	2.627459962270944	0.0
33	1.8293762705018197E-4	0.0	0.0	2.712782071527149	0.0
34	1.8293762705018197E-4	0.0	0.0	2.8040679474251893	0.0
35	1.8293762705018197E-4	0.0	0.0	2.901939577897037	0.0
36	1.8293762705018197E-4	0.0	0.0	2.993444978947538	0.0
37	1.8293762705018197E-4	0.0	0.0	3.0834868789816374	0.0
38	1.8293762705018197E-4	0.0	0.0	3.2041891253093477	0.0
39	1.8293762705018197E-4	0.0	0.0	3.3468804744084895	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	14925	0.0	41.351757	1
TACGGGA	1000	0.0	40.95	4
CGTAAGG	410	0.0	40.609753	2
AGGGCGA	3490	0.0	39.777935	6
CGCGTAA	40	3.4605546E-7	39.375	31
ATAGTCG	75	0.0	39.0	1
ACGGGAT	1100	0.0	38.863636	5
GCGAGAC	2025	0.0	38.666668	21
TCGACGC	35	6.25145E-6	38.571426	36
GACACGA	2020	0.0	38.5396	25
CACGACC	2050	0.0	38.195126	27
ACACGAC	2045	0.0	38.06846	26
TCAAGCG	2040	0.0	38.05147	17
CACGTGA	1880	0.0	37.81915	43
GGGCGAT	6920	0.0	37.814304	7
CGTTAGG	460	0.0	37.663044	2
AACGGGA	1190	0.0	37.62605	4
AACACGT	1910	0.0	37.578533	41
CGACCAA	2075	0.0	37.30121	29
TAAGGGA	3525	0.0	37.021275	4
>>END_MODULE