FastQCFastQC Report
Thu 26 May 2016
SRR1547290_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547290_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1449661
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94650.6529112668410063No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC77480.5344697829354588No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT45390.3131076851760515No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATG23900.16486613077126308No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTC15820.1091289618745348No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT53500.070.803741
ATTGCGG2150.070.697672
ACGGGAT5100.069.8039255
TAGGGTA5250.067.8095255
CGATAGG3200.067.52
GGGCGAT6800.067.058827
GTAGGGT5850.066.324794
CGAGGGA10100.066.138614
GCTAGCG1300.064.615391
CGTAAGG2050.064.390242
TAGGGAC12750.064.05
GCGATAA750.064.09
GTACGTA251.2356351E-464.08
TGCGTAG1000.064.01
GATACCT10350.063.381645
TACGGGA5350.062.8037384
TACGCGG1150.062.6086922
CGGGATC4850.061.855676
GGGACCG4550.060.659347
ATAGCGG3300.060.606062
Produced by FastQC (version 0.11.5)