##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547290_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1449661
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.900358083717503	31.0	31.0	34.0	30.0	34.0
2	32.31664644354784	33.0	31.0	34.0	31.0	34.0
3	32.4372222195396	34.0	31.0	34.0	30.0	34.0
4	35.99828097741472	37.0	35.0	37.0	35.0	37.0
5	35.94622397926136	37.0	35.0	37.0	35.0	37.0
6	35.99821751430162	37.0	35.0	37.0	35.0	37.0
7	36.21589944131766	37.0	35.0	37.0	35.0	37.0
8	36.16947755371773	37.0	36.0	37.0	35.0	37.0
9	37.88360658112483	39.0	38.0	39.0	35.0	39.0
10-11	37.523902484787826	39.0	37.0	39.0	35.0	39.0
12-13	36.75646789145876	39.0	35.0	39.0	33.0	39.0
14-15	37.7957470746609	40.0	35.0	41.0	33.0	41.0
16-17	37.96997642897202	40.0	35.0	41.0	33.0	41.0
18-19	37.82216945892867	40.0	36.0	41.0	33.5	41.0
20-21	37.57765332722616	40.0	35.0	41.0	33.0	41.0
22-23	37.38169165066867	39.0	35.0	41.0	33.0	41.0
24-25	37.38114083223595	39.0	35.0	41.0	33.0	41.0
26-27	37.349777292760166	39.0	35.0	41.0	33.0	41.0
28-29	37.23090053467673	39.0	35.0	41.0	33.0	41.0
30-31	37.07261145881692	39.0	35.0	41.0	32.5	41.0
32-33	36.691460624242495	39.0	35.0	41.0	31.5	41.0
34-35	36.312720353241204	39.0	35.0	41.0	30.0	41.0
36-37	36.107439601396464	39.0	35.0	41.0	30.0	41.0
38-39	35.99765910788798	39.0	35.0	41.0	30.0	41.0
40-41	35.8728371667583	38.5	35.0	41.0	29.0	41.0
42-43	35.84085451702157	38.0	35.0	41.0	29.0	41.0
44-45	35.764562887461274	38.0	35.0	41.0	29.0	41.0
46-47	35.62820687043384	38.0	35.0	40.5	29.0	41.0
48-49	35.54700857648788	38.0	35.0	40.0	28.0	41.0
50-51	35.31349570692734	37.5	35.0	40.0	27.5	41.0
52-53	35.09424237804562	37.0	34.5	40.0	27.0	41.0
54-55	34.93769612343851	37.0	34.0	40.0	26.5	41.0
56-57	34.67205022415585	36.0	34.0	40.0	26.0	41.0
58-59	34.40333084769473	36.0	34.0	40.0	25.5	41.0
60-61	34.01951145819609	35.0	34.0	39.0	25.0	41.0
62-63	33.51701432265888	35.0	33.0	39.0	23.0	40.0
64-65	33.01734543455332	35.0	33.0	38.0	21.0	40.0
66-67	32.64172761769821	35.0	33.0	37.5	19.5	40.0
68-69	32.078388671558386	35.0	32.0	37.0	17.5	39.5
70-71	31.68553061715808	35.0	32.0	36.0	16.0	39.0
72-73	31.40485568695026	35.0	31.5	36.0	13.0	38.5
74-75	31.091443102904748	35.0	31.0	35.5	12.5	37.5
76-77	29.22766874462374	33.0	28.0	34.5	10.5	35.5
78-79	30.194460635969374	34.0	30.0	35.0	10.5	36.0
80-81	30.308423831502676	34.0	31.0	35.0	10.0	36.0
82-83	30.110010202385247	34.0	30.5	35.0	10.0	36.0
84-85	29.87547605957531	34.0	30.0	35.0	10.0	36.0
86	29.437478831257792	34.0	29.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	5.0
10	12.0
11	17.0
12	41.0
13	93.0
14	261.0
15	767.0
16	1883.0
17	4106.0
18	8055.0
19	13268.0
20	15484.0
21	15972.0
22	14290.0
23	12764.0
24	12640.0
25	14023.0
26	16374.0
27	20076.0
28	24886.0
29	31033.0
30	38616.0
31	46757.0
32	54272.0
33	66546.0
34	160002.0
35	142039.0
36	119623.0
37	205269.0
38	284151.0
39	125981.0
40	354.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.782227707029435	22.822025287291307	27.473112679447127	13.922634326232133
2	31.280485575593193	26.583801316307742	28.24287885236617	13.892834255732891
3	28.44044228271299	25.99511196065839	31.5368213672024	14.027624389426219
4	25.932338664004895	26.0049763358468	33.288679215347585	14.774005784800723
5	26.876973306172964	29.27394749531097	28.988777376227958	14.860301822288108
6	22.546995469975396	40.42427850373294	26.64181487947872	10.386911146812945
7	81.76021842347969	4.399511334029128	11.153021292564262	2.687248949926914
8	81.36171146219702	6.219040175599674	9.19870231730039	3.2205460449029117
9	76.819063215469	6.315890404722207	10.486313696788422	6.378732683020375
10-11	40.70751713676508	25.742225251282886	21.436115064142584	12.114142547809454
12-13	30.609983989360273	23.531708447699152	28.425818174042067	17.43248938889851
14-15	16.63019837051559	29.79506933000198	35.52358103032364	18.051151269158787
16-17	22.81961092972771	25.662723905795907	31.608010424506144	19.909654739970243
18-19	23.64601103292425	26.97644483779311	29.567498884221898	19.810045245060742
20-21	26.306908994585633	28.210940350882037	29.400114923420027	16.08203573111231
22-23	22.158870246216182	24.518008003250415	31.608286351084843	21.71483539944856
24-25	20.726052504689026	27.22426139628506	32.36208327326182	19.687602825764092
26-27	18.8365072937742	31.51053936058154	29.281018113890074	20.371935231754183
28-29	17.53658269071183	28.59544403829585	32.929284846595166	20.938688424397153
30-31	21.673722339222756	28.62251933383046	29.3266149810197	20.37714334592708
32-33	26.058644055403295	27.66922749525579	27.407269699605628	18.864858749735284
34-35	20.440675440671992	28.61199963301765	29.08414449999	21.863180426320362
36-37	22.378887201904448	30.297980010499003	27.80132734480682	19.521805442789727
38-39	20.406529526558277	31.00176524028721	27.154693407631164	21.437011825523346
40-41	21.042402327164762	26.440767876075853	28.12095379540458	24.3958760013548
42-43	20.762957684589708	26.025394902670346	28.676083580919954	24.53556383181999
44-45	19.65628515908202	29.239318709684543	27.308005112919503	23.796391018313937
46-47	20.716257111145296	29.466268320662554	28.109571824033342	21.707902744158808
48-49	21.16336164110092	25.98317813612976	31.073713095682372	21.779747127086953
50-51	19.17662129284019	28.749790468254304	28.758033774792864	23.315554464112644
52-53	21.47850428479486	25.442293060239603	30.527274997395942	22.5519276575696
54-55	20.93172127828506	25.46505700298208	31.679406426743906	21.923815291988955
56-57	22.152179026682788	25.372828544052712	29.399218162039265	23.075774267225235
58-59	22.58038258599769	29.1678537258021	27.370364519704953	20.881399168495253
60-61	23.133718848751535	30.01163720345653	26.226648851007234	20.627995096784694
62-63	21.707833762514134	27.836197566189615	29.584951240324465	20.87101743097179
64-65	19.647869398431773	31.563275827934945	28.16082518602625	20.62802958760703
66-67	19.587338005230187	31.122517609289346	25.544558348469053	23.745586037011414
68-69	18.049564691331284	32.88406737851125	25.744087755689087	23.322280174468375
70-71	18.77228538258255	31.189567767912635	28.099017632398194	21.939129217106622
72-73	20.78361768716962	31.891145585071268	26.390204330529688	20.93503239722942
74-75	19.432853611982388	32.78259537919555	25.276840585488607	22.507710423333453
76-77	22.28051937659908	29.748403247379905	26.059954706652107	21.911122669368908
78-79	18.872860620517486	30.732184972900562	27.47007748708146	22.924876919500488
80-81	17.281488568706752	30.253521340506506	28.663390958299907	23.801599132486835
82-83	18.21367202401113	30.09676055298446	28.884028748790236	22.80553867421418
84-85	17.592699258654264	29.752059274547637	27.694026396516154	24.96121507028195
86	18.135688274706983	30.345508363679507	28.148236035873214	23.370567325740293
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	531.0
1	812.5
2	3376.0
3	3778.5
4	2054.0
5	2646.0
6	3488.5
7	3894.0
8	3968.0
9	4032.5
10	3865.5
11	3705.0
12	3642.0
13	3567.0
14	3552.0
15	3645.5
16	4048.5
17	4177.5
18	3992.0
19	3993.0
20	4027.5
21	4056.0
22	4670.0
23	5719.0
24	6237.5
25	7122.0
26	9258.0
27	11819.5
28	13173.0
29	13300.0
30	16115.0
31	20404.0
32	22694.5
33	24717.5
34	28603.5
35	33704.0
36	36125.0
37	38597.0
38	42707.5
39	47853.0
40	53628.5
41	63231.5
42	69564.5
43	68563.0
44	72193.0
45	75259.5
46	75648.5
47	76371.0
48	74561.0
49	66516.5
50	60052.0
51	55582.5
52	50258.0
53	48627.5
54	45508.0
55	41937.0
56	38933.0
57	37156.0
58	34245.0
59	27287.0
60	20335.5
61	15988.5
62	13491.0
63	10278.5
64	8121.0
65	6959.5
66	4868.0
67	3538.5
68	2591.5
69	1799.5
70	1317.0
71	1079.0
72	962.5
73	647.5
74	371.5
75	208.5
76	132.5
77	90.5
78	49.5
79	60.0
80	54.5
81	51.5
82	45.5
83	23.5
84	6.0
85	2.0
86	2.0
87	3.0
88	4.5
89	3.0
90	1.5
91	1.5
92	0.5
93	0.0
94	1.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1449661.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.097053167800155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.3812477834362	16.747104050134716
2	8.160808379986193	3.4433801656959555
3	2.7073232059900576	1.7134962485757428
4	1.3671457173222845	1.1537098354591406
5	0.8566961204351966	0.9036881800734734
6	0.5428203525873201	0.6871145903459231
7	0.4153535265060543	0.6133914802492064
8	0.34501326713498104	0.5823010592274507
9	0.2666990020122479	0.5063906722726471
>10	3.429072297773126	17.93211848342872
>50	1.4869143556524997	22.918408395820702
>100	1.0336430885931291	29.881825053528754
>500	0.003301319350345137	0.5090580349529905
>1k	0.003301319350345137	1.209160835246801
>5k	6.602638700690274E-4	1.1988529149877993
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9465	0.6529112668410063	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC	7748	0.5344697829354588	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT	4539	0.3131076851760515	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATG	2390	0.16486613077126308	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTC	1582	0.1091289618745348	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.898164467416864E-5	0.0	0.0	0.09816088037134199	0.0
2	6.898164467416864E-5	0.0	0.0	0.36360224907754296	0.0
3	6.898164467416864E-5	0.0	0.0	0.5239155913003108	0.0
4	6.898164467416864E-5	0.0	0.0	0.8778604101234703	0.0
5	6.898164467416864E-5	0.0	0.0	1.8063533474377802	0.0
6	6.898164467416864E-5	0.0	0.0	2.260804422551203	0.0
7	6.898164467416864E-5	0.0	0.0	2.6617947230421457	0.0
8	6.898164467416864E-5	0.0	0.0	3.278214699850517	0.0
9	6.898164467416864E-5	0.0	0.0	3.4384590604286105	0.0
10	6.898164467416864E-5	0.0	0.0	4.268860099016253	0.0
11	6.898164467416864E-5	0.0	0.0	4.780221030985865	0.0
12	6.898164467416864E-5	0.0	0.0	5.318622767667751	0.0
13	6.898164467416864E-5	0.0	0.0	5.468588863189394	0.0
14	6.898164467416864E-5	0.0	0.0	5.542123296412058	0.0
15	6.898164467416864E-5	0.0	0.0	5.691192630552936	0.0
16	6.898164467416864E-5	0.0	0.0	5.897792656352071	0.0
17	6.898164467416864E-5	0.0	0.0	6.117913084507343	0.0
18	6.898164467416864E-5	0.0	0.0	6.371282665395565	0.0
19	6.898164467416864E-5	0.0	0.0	6.6152017609634255	0.0
20	6.898164467416864E-5	0.0	0.0	6.816697145056672	0.0
21	6.898164467416864E-5	0.0	0.0	7.056546323588757	0.0
22	6.898164467416864E-5	0.0	0.0	7.334266425046959	0.0
23	6.898164467416864E-5	0.0	0.0	7.588532767315945	0.0
24	6.898164467416864E-5	0.0	0.0	7.780646647733505	0.0
25	6.898164467416864E-5	0.0	0.0	7.953997520799691	0.0
26	6.898164467416864E-5	0.0	0.0	8.12824515524664	0.0
27	6.898164467416864E-5	0.0	0.0	8.34602020748299	0.0
28	6.898164467416864E-5	0.0	0.0	8.528752584224863	0.0
29	6.898164467416864E-5	0.0	0.0	8.763635084340407	0.0
30	6.898164467416864E-5	0.0	0.0	9.000518052151502	0.0
31	6.898164467416864E-5	0.0	0.0	9.227191736550822	0.0
32	6.898164467416864E-5	0.0	0.0	9.414752828419886	0.0
33	6.898164467416864E-5	0.0	0.0	9.612316258766704	0.0
34	6.898164467416864E-5	0.0	0.0	9.821882495286829	0.0
35	6.898164467416864E-5	0.0	0.0	10.050487665737023	0.0
36	1.379632893483373E-4	0.0	0.0	10.263365021201508	0.0
37	1.379632893483373E-4	0.0	0.0	10.485623880341679	0.0
38	1.379632893483373E-4	0.0	0.0	10.736786048600328	0.0
39	1.379632893483373E-4	0.0	0.0	11.142880990797158	0.0
40	1.379632893483373E-4	0.0	0.0	11.396112608396033	0.0
41	1.379632893483373E-4	0.0	0.0	11.660726197366143	0.0
42	1.379632893483373E-4	0.0	0.0	11.921752740813197	0.0
43	1.379632893483373E-4	0.0	0.0	12.134147224764963	0.0
44	1.379632893483373E-4	0.0	0.0	12.35419867127556	0.0
45	1.379632893483373E-4	0.0	0.0	12.565282503978516	0.0
46	1.379632893483373E-4	0.0	0.0	12.80713215020615	0.0
47	1.379632893483373E-4	0.0	0.0	13.0561558874799	0.0
48	1.379632893483373E-4	0.0	0.0	13.28434716806205	0.0
49	1.379632893483373E-4	0.0	0.0	13.526541722513057	0.0
50	1.379632893483373E-4	0.0	0.0	13.763493671968826	0.0
51	2.0694493402250596E-4	0.0	0.0	14.004101648592327	0.0
52	2.0694493402250596E-4	0.0	0.0	14.31989961791067	0.0
53	2.0694493402250596E-4	0.0	0.0	14.565060383082665	0.0
54	2.0694493402250596E-4	0.0	0.0	14.819671633575021	0.0
55	2.0694493402250596E-4	0.0	0.0	15.06469443545767	0.0
56	2.0694493402250596E-4	0.0	0.0	15.325445052326026	0.0
57	2.0694493402250596E-4	0.0	0.0	15.589644751428093	0.0
58	2.0694493402250596E-4	0.0	0.0	15.824941141411681	0.0
59	2.759265786966746E-4	0.0	0.0	16.064100503497023	0.0
60	2.759265786966746E-4	0.0	0.0	16.33243910127954	0.0
61	2.759265786966746E-4	0.0	0.0	16.608158735042192	0.0
62	2.759265786966746E-4	0.0	0.0	16.939960445924942	0.0
63	2.759265786966746E-4	0.0	0.0	17.268795946086705	0.0
64	2.759265786966746E-4	0.0	0.0	17.559208670164956	0.0
65	2.759265786966746E-4	0.0	0.0	17.83230700142999	0.0
66	2.759265786966746E-4	0.0	0.0	18.085745563962885	0.0
67	2.759265786966746E-4	0.0	0.0	18.34594432767385	0.0
68	3.4490822337084325E-4	0.0	0.0	18.654982095814123	0.0
69	3.4490822337084325E-4	0.0	0.0	19.101086391921974	0.0
70	3.4490822337084325E-4	0.0	0.0	19.389222721725975	0.0
71	3.4490822337084325E-4	0.0	0.0	19.667839584564945	0.0
72	3.4490822337084325E-4	0.0	0.0	19.936936980438876	0.0
73	3.4490822337084325E-4	0.0	0.0	20.198239450464627	0.0
74	3.4490822337084325E-4	0.0	0.0	20.50741518189425	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	5350	0.0	70.80374	1
ATTGCGG	215	0.0	70.69767	2
ACGGGAT	510	0.0	69.803925	5
TAGGGTA	525	0.0	67.809525	5
CGATAGG	320	0.0	67.5	2
GGGCGAT	680	0.0	67.05882	7
GTAGGGT	585	0.0	66.32479	4
CGAGGGA	1010	0.0	66.13861	4
GCTAGCG	130	0.0	64.61539	1
CGTAAGG	205	0.0	64.39024	2
TAGGGAC	1275	0.0	64.0	5
GCGATAA	75	0.0	64.0	9
GTACGTA	25	1.2356351E-4	64.0	8
TGCGTAG	100	0.0	64.0	1
GATACCT	1035	0.0	63.38164	5
TACGGGA	535	0.0	62.803738	4
TACGCGG	115	0.0	62.608692	2
CGGGATC	485	0.0	61.85567	6
GGGACCG	455	0.0	60.65934	7
ATAGCGG	330	0.0	60.60606	2
>>END_MODULE