FastQCFastQC Report
Thu 26 May 2016
SRR1547289_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547289_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2110197
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138120.6545360456867297No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG150.001309943480.000011
CGTAAGG4400.078.1818162
GCGATAT1900.075.789479
TTTAGCG1600.075.000011
CGTTTTT75200.072.6063841
GCGTAAG2050.072.195121
ACGGGTA1200.070.000015
ATCGTAG1150.069.5652161
GCGGGAT16650.068.468475
GGCGATA4100.068.292698
CGTAGGG11350.065.198243
TTTGCGG7750.065.032262
CGTATGG2250.064.02
ACGGGAC6600.063.6363645
TTACGGG7300.063.5616463
TTGCGGG16700.063.473053
CGATAGG5500.063.2727282
ACGGGAT11350.062.731285
AGGGATT40150.062.665016
GCGGGGT10150.062.6600955
Produced by FastQC (version 0.11.5)