##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547289_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2110197
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0086939750175	33.0	31.0	34.0	31.0	34.0
2	32.43654218065896	34.0	31.0	34.0	31.0	34.0
3	32.53341275719755	34.0	31.0	34.0	31.0	34.0
4	36.065687705934565	37.0	35.0	37.0	35.0	37.0
5	36.02410533234575	37.0	35.0	37.0	35.0	37.0
6	36.080201990619834	37.0	35.0	37.0	35.0	37.0
7	36.343797285277155	37.0	37.0	37.0	35.0	37.0
8	36.34676193739258	37.0	37.0	37.0	35.0	37.0
9	38.15769475551335	39.0	39.0	39.0	37.0	39.0
10-11	37.63669766377262	39.0	37.5	39.0	35.0	39.0
12-13	36.88364356503208	39.0	35.0	39.0	33.5	39.0
14-15	37.95548022293653	40.0	36.0	41.0	33.0	41.0
16-17	38.1824448143941	40.0	36.0	41.0	34.0	41.0
18-19	38.06461410948836	40.0	36.0	41.0	34.0	41.0
20-21	37.87434277463194	40.0	35.0	41.0	34.0	41.0
22-23	37.68979033711071	40.0	35.0	41.0	33.0	41.0
24-25	37.66147828851998	40.0	35.0	41.0	34.0	41.0
26-27	37.65352050069259	40.0	35.0	41.0	34.0	41.0
28-29	37.59519490360378	40.0	35.0	41.0	33.5	41.0
30-31	37.442920968990094	40.0	35.0	41.0	33.0	41.0
32-33	37.1399236658947	40.0	35.0	41.0	33.0	41.0
34-35	36.84247655550643	40.0	35.0	41.0	31.5	41.0
36-37	36.654967521989654	39.0	35.0	41.0	31.0	41.0
38-39	36.56394521459371	39.0	35.0	41.0	31.0	41.0
40-41	36.42864765706709	39.0	35.0	41.0	31.0	41.0
42-43	36.3944238381535	39.0	35.0	41.0	31.0	41.0
44-45	36.26770273107203	39.0	35.0	41.0	30.5	41.0
46-47	36.15062622115376	38.5	35.0	41.0	30.0	41.0
48-49	36.05955036425509	38.0	35.0	40.5	30.0	41.0
50-51	35.8410634173018	38.0	35.0	40.0	29.5	41.0
52-53	35.645435710504756	37.5	35.0	40.0	29.0	41.0
54-55	35.44727909289986	37.0	35.0	40.0	29.0	41.0
56-57	35.157021832558755	37.0	34.5	40.0	28.5	41.0
58-59	34.87534031182871	36.0	34.0	40.0	28.0	41.0
60-61	34.493833751066845	36.0	34.0	39.0	26.5	41.0
62-63	34.07550527273047	35.0	33.5	39.0	26.0	40.0
64-65	33.66637972663216	35.0	33.0	38.5	25.5	40.0
66-67	33.33637665108992	35.0	33.0	37.5	24.5	40.0
68-69	32.847417089494485	35.0	33.0	37.0	22.5	39.5
70-71	32.51367242015793	35.0	33.0	36.5	21.5	39.0
72-73	32.2422408903055	35.0	33.0	36.0	20.0	39.0
74-75	31.953817345015654	35.0	32.5	35.5	20.0	37.5
76-77	30.022663523832136	33.0	29.5	34.5	17.5	36.0
78-79	31.029552927996768	34.0	31.0	35.0	18.0	36.0
80-81	31.174092276692647	35.0	32.0	35.0	18.0	36.0
82-83	30.969426077280936	34.5	32.0	35.0	16.0	36.0
84-85	30.73688309669666	34.0	31.0	35.0	15.0	36.0
86	30.332663253715175	34.0	31.0	35.0	12.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	5.0
11	7.0
12	30.0
13	68.0
14	245.0
15	763.0
16	1892.0
17	4434.0
18	9710.0
19	15210.0
20	17794.0
21	18020.0
22	16182.0
23	14329.0
24	14421.0
25	16343.0
26	19531.0
27	24238.0
28	29722.0
29	37601.0
30	48247.0
31	60341.0
32	71904.0
33	90987.0
34	227548.0
35	211259.0
36	174413.0
37	321300.0
38	465665.0
39	197576.0
40	407.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.450994385832225	24.199825893032738	32.031748694553166	14.317431026581879
2	30.96118514053427	24.823464349537034	30.914886145701086	13.300464364227604
3	28.792193335503747	24.533207089195937	33.40157340760128	13.273026167699035
4	26.114623421415157	27.346830651356246	32.67467444982625	13.863871477402348
5	23.86876675495226	31.33437304668711	30.939196672159046	13.857663526201582
6	22.971551945150146	39.12023379807667	27.368961286552867	10.539252970220316
7	88.61627611071383	3.0177277287381226	6.912766912283545	1.4532292482644986
8	89.43890072822585	2.1463398914888043	6.634688609641659	1.7800707706436887
9	85.30435783957611	4.675345477223217	7.619904681885151	2.400392001315517
10-11	39.782470546588776	27.34988723801617	20.425723285551065	12.441918929843991
12-13	29.927821904779506	24.580051056844457	28.35282677399314	17.139300264382896
14-15	16.191402982754692	30.437821682051485	35.4377103180414	17.933065017152426
16-17	22.044576880736727	26.407439684541302	31.781487699963556	19.76649573475841
18-19	23.829670879069585	27.685874825904882	29.947298759310147	18.537155535715385
20-21	25.38871963138987	28.085861177890024	30.002032985545902	16.52338620517421
22-23	21.28187557844125	24.190442882820893	32.495662727224044	22.032018811513808
24-25	19.76483712184218	26.87597413890741	34.241068487918426	19.118120251331984
26-27	18.560423505483136	32.55565712585128	29.692369954084857	19.191549414580724
28-29	16.95062119792607	27.909550624894266	34.929582403917735	20.210245773261928
30-31	22.038984985761992	27.687865161404364	31.02523129357117	19.247918559262477
32-33	24.372913998076957	28.311551006849122	28.899647758005532	18.415887237068386
34-35	19.38868740691035	28.580980827856354	30.787172951151003	21.243158814082285
36-37	21.964608043703976	29.258974399072695	29.725139406415607	19.051278150807722
38-39	20.081892828015583	30.100625676180943	28.79010822212334	21.027373273680137
40-41	20.68657570833434	27.370975316522582	28.834985548742605	23.107463426400475
42-43	20.5531047575179	26.80526983973534	29.01698277459403	23.624642628152728
44-45	19.683730950238296	30.135124824838627	28.320412738715866	21.860731486207214
46-47	20.963492981934863	28.738454277017738	28.88116607122463	21.41688666982277
48-49	20.8516787769104	27.338324336542986	30.610933481565937	21.199063404980674
50-51	19.019195838113692	29.979215210712557	29.028308731364895	21.97328021980886
52-53	21.206645635454887	26.547545087022677	30.943129954217547	21.302679323304886
54-55	21.13063377495087	26.92476105311495	31.14910598394368	20.795499187990504
56-57	21.205958495818162	25.97233812767244	30.1732492274418	22.648454149067597
58-59	22.96790299673443	27.90651773270458	28.603206240933904	20.52237302962709
60-61	22.93646043473666	29.033189792232665	27.469188895633916	20.561160877396755
62-63	21.576895427298968	26.825978806718048	30.721776213310893	20.875349552672095
64-65	19.282583569211784	30.37154824881279	29.744853205648575	20.601014976326855
66-67	18.98161640832586	29.291246267528575	27.016103235859024	24.711034088286542
68-69	18.067886552772087	31.324421369189704	27.047806437029337	23.559885641008872
70-71	19.19391886160392	30.004852627503496	29.184573762544446	21.61665474834814
72-73	21.34158564342571	29.877305294245037	27.845338610565744	20.935770451763506
74-75	20.389826163149696	31.068260451512348	26.514396523168216	22.02751686216974
76-77	23.06322585047747	28.892231388822935	27.400474931961327	20.64406782873826
78-79	18.52997611123511	30.317738106916085	29.294989993825222	21.85729578802358
80-81	17.343451819901176	29.593848346860508	30.34403896887352	22.718660864364796
82-83	18.58101400011468	28.57472548771513	30.525894027903554	22.318366484266637
84-85	17.03805379308188	28.45916755639402	29.552999080180665	24.949779570343434
86	18.297628136140844	28.20608691984682	30.40744537121416	23.08883957279818
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	821.0
1	1078.5
2	4661.0
3	5102.0
4	2570.5
5	3504.5
6	4812.5
7	5539.0
8	5833.0
9	6034.0
10	5667.5
11	5123.5
12	4926.5
13	4898.5
14	4797.0
15	5002.0
16	5239.5
17	5724.5
18	6014.5
19	5815.5
20	6196.0
21	6613.0
22	6782.0
23	7707.5
24	8711.5
25	10218.5
26	12537.0
27	16225.0
28	19449.0
29	20044.0
30	23589.0
31	30127.0
32	35395.0
33	40811.5
34	44314.5
35	49278.0
36	53880.0
37	58434.5
38	67190.5
39	75886.5
40	87970.5
41	98361.0
42	104662.0
43	108162.0
44	111127.5
45	114514.5
46	117416.5
47	117425.0
48	114443.5
49	104631.0
50	95328.0
51	87513.0
52	75016.0
53	66318.0
54	57897.0
55	54427.0
56	50715.0
57	46068.0
58	42394.5
59	33099.5
60	22900.5
61	16606.5
62	12809.5
63	8876.0
64	7023.0
65	5512.5
66	3563.0
67	2442.5
68	1539.0
69	1108.5
70	694.5
71	489.0
72	351.0
73	148.5
74	91.5
75	94.0
76	74.5
77	41.5
78	25.5
79	28.0
80	21.0
81	12.5
82	11.0
83	10.5
84	12.0
85	15.0
86	16.0
87	16.0
88	11.0
89	3.5
90	0.5
91	0.5
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2110197.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.43869581184062
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.0654651951758	14.394253661436773
2	9.315176402715238	3.43519408246604
3	3.2147809722140077	1.77829105345042
4	1.571742556707072	1.1592353159058542
5	0.9007033161327257	0.8303897231443721
6	0.6169774579911582	0.6825755802396984
7	0.44495070060992314	0.5743017433868218
8	0.3441831459839999	0.5077030665889045
9	0.27543167445268396	0.4570740775961058
>10	2.566306185436518	10.982687465251665
>50	1.113498763596526	14.977686163303385
>100	1.564576020374489	48.57204724593824
>500	0.0038797553811738706	0.5019556989063475
>1k	0.0020692028699593975	0.48788663181111025
>5k	0.0	0.0
>10k+	2.586503587449247E-4	0.6587184905742967
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13812	0.6545360456867297	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.477788092770485E-5	0.0	0.0	0.0701356318865016	0.0
2	9.477788092770485E-5	0.0	0.0	0.2584118923493873	0.0
3	9.477788092770485E-5	0.0	0.0	0.3817179154363313	0.0
4	9.477788092770485E-5	0.0	0.0	0.5873859170494509	0.0
5	9.477788092770485E-5	0.0	0.0	1.0456843602753676	0.0
6	9.477788092770485E-5	0.0	0.0	1.3896332901620085	0.0
7	9.477788092770485E-5	0.0	0.0	1.6269571040049815	0.0
8	9.477788092770485E-5	0.0	0.0	2.0739769794005016	0.0
9	9.477788092770485E-5	0.0	0.0	2.210172794293613	0.0
10	9.477788092770485E-5	0.0	0.0	2.471996690356398	0.0
11	9.477788092770485E-5	0.0	0.0	2.9895313091621305	0.0
12	9.477788092770485E-5	0.0	0.0	3.3429580271415418	0.0
13	9.477788092770485E-5	0.0	0.0	3.4650319377764256	0.0
14	9.477788092770485E-5	0.0	0.0	3.5166858828820247	0.0
15	9.477788092770485E-5	0.0	0.0	3.596915359087327	0.0
16	9.477788092770485E-5	0.0	0.0	3.7724913835059	0.0
17	9.477788092770485E-5	0.0	0.0	4.004507636016922	0.0
18	9.477788092770485E-5	0.0	0.0	4.279079157064483	0.0
19	9.477788092770485E-5	0.0	0.0	4.466360249777628	0.0
20	9.477788092770485E-5	0.0	0.0	4.6658676891304465	0.0
21	9.477788092770485E-5	0.0	0.0	4.9250851934677184	0.0
22	9.477788092770485E-5	0.0	0.0	5.199941048158063	0.0
23	9.477788092770485E-5	0.0	0.0	5.483895579417466	0.0
24	9.477788092770485E-5	0.0	0.0	5.691648694410996	0.0
25	9.477788092770485E-5	0.0	0.0	5.881488789909189	0.0
26	9.477788092770485E-5	0.0	0.0	6.062846265064352	0.0
27	1.4216682139155728E-4	0.0	0.0	6.263159316405056	0.0
28	1.4216682139155728E-4	0.0	0.0	6.465652259007097	0.0
29	1.4216682139155728E-4	0.0	0.0	6.722026426916539	0.0
30	1.4216682139155728E-4	0.0	0.0	6.99166949815586	0.0
31	1.4216682139155728E-4	0.0	0.0	7.233921761807073	0.0
32	1.4216682139155728E-4	0.0	0.0	7.442954378193126	0.0
33	1.4216682139155728E-4	0.0	0.0	7.655304220411649	0.0
34	1.4216682139155728E-4	0.0	0.0	7.888410418553339	0.0
35	1.4216682139155728E-4	0.0	0.0	8.154025429853231	0.0
36	1.4216682139155728E-4	0.0	0.0	8.380781509972765	0.0
37	1.4216682139155728E-4	0.0	0.0	8.630094725753093	0.0
38	1.4216682139155728E-4	0.0	0.0	8.90509274726483	0.0
39	1.4216682139155728E-4	0.0	0.0	9.314580581812978	0.0
40	1.4216682139155728E-4	0.0	0.0	9.59569177664455	0.0
41	1.4216682139155728E-4	0.0	0.0	9.864387069074594	0.0
42	1.4216682139155728E-4	0.0	0.0	10.137442144027311	0.0
43	1.4216682139155728E-4	0.0	0.0	10.405995269635962	0.0
44	1.4216682139155728E-4	0.0	0.0	10.658151821844122	0.0
45	1.4216682139155728E-4	0.0	0.0	10.926088891226744	0.0
46	1.4216682139155728E-4	0.0	0.0	11.203551137642599	0.0
47	1.4216682139155728E-4	0.0	0.0	11.502575351969508	0.0
48	1.4216682139155728E-4	0.0	0.0	11.783354824217835	0.0
49	1.4216682139155728E-4	0.0	0.0	12.078682701188562	0.0
50	1.4216682139155728E-4	0.0	0.0	12.372588909945375	0.0
51	1.4216682139155728E-4	0.0	0.0	12.660524112203742	0.0
52	1.4216682139155728E-4	0.0	0.0	13.015372498397069	0.0
53	1.4216682139155728E-4	0.0	0.0	13.328755561684526	0.0
54	1.4216682139155728E-4	0.0	0.0	13.643181181662186	0.0
55	1.4216682139155728E-4	0.0	0.0	13.945190899238318	0.0
56	1.4216682139155728E-4	0.0	0.0	14.250233509004136	0.0
57	1.4216682139155728E-4	0.0	0.0	14.574610806479205	0.0
58	1.4216682139155728E-4	0.0	0.0	14.878705637435747	0.0
59	1.4216682139155728E-4	0.0	0.0	15.197159317352835	0.0
60	1.4216682139155728E-4	0.0	0.0	15.546415808571426	0.0
61	1.4216682139155728E-4	0.0	0.0	15.896335744956513	0.0
62	1.895557618554097E-4	0.0	0.0	16.280280940594647	0.0
63	1.895557618554097E-4	0.0	0.0	16.66474741457788	0.0
64	1.895557618554097E-4	0.0	0.0	17.040257378813447	0.0
65	1.895557618554097E-4	0.0	0.0	17.376576689285407	0.0
66	2.3694470231926213E-4	0.0	0.0	17.682614466800967	0.0
67	2.8433364278311455E-4	0.0	0.0	18.010498545870362	0.0
68	3.31722583246967E-4	0.0	0.0	18.400556914828332	0.0
69	3.31722583246967E-4	0.0	0.0	18.930602213916522	0.0
70	3.31722583246967E-4	0.0	0.0	19.28255987474155	0.0
71	3.31722583246967E-4	0.0	0.0	19.64480093564724	0.0
72	3.31722583246967E-4	0.0	0.0	19.989508088581303	0.0
73	3.791115237108194E-4	0.0	0.0	20.339712358609173	0.0
74	3.791115237108194E-4	0.0	0.0	20.732851008697292	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGCG	15	0.0013099434	80.00001	1
CGTAAGG	440	0.0	78.181816	2
GCGATAT	190	0.0	75.78947	9
TTTAGCG	160	0.0	75.00001	1
CGTTTTT	7520	0.0	72.606384	1
GCGTAAG	205	0.0	72.19512	1
ACGGGTA	120	0.0	70.00001	5
ATCGTAG	115	0.0	69.565216	1
GCGGGAT	1665	0.0	68.46847	5
GGCGATA	410	0.0	68.29269	8
CGTAGGG	1135	0.0	65.19824	3
TTTGCGG	775	0.0	65.03226	2
CGTATGG	225	0.0	64.0	2
ACGGGAC	660	0.0	63.636364	5
TTACGGG	730	0.0	63.561646	3
TTGCGGG	1670	0.0	63.47305	3
CGATAGG	550	0.0	63.272728	2
ACGGGAT	1135	0.0	62.73128	5
AGGGATT	4015	0.0	62.66501	6
GCGGGGT	1015	0.0	62.660095	5
>>END_MODULE