FastQCFastQC Report
Thu 26 May 2016
SRR1547288_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547288_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3358938
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222480.6623522077513786No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT123150.072.7568051
TACGGGA17100.069.941524
CGTTAGG7100.068.1690142
ACGGGAT17700.064.8587655
GTAGGGA52750.064.758294
GCGGGAT30200.064.503315
GGCGATA5300.062.6415148
TAGGGAT60950.062.477445
TAGGGAC46550.062.1267475
TCGTTAG2000.062.01
GTACGGG13950.061.9354863
AGGGATT70300.061.906126
CGGGATT17600.061.5909086
TACGCGG2600.061.5384642
CGTAAGG6150.061.138212
CGGGAAT8450.061.065096
TTGCGGG21600.060.3703733
GGGCGAT22700.060.0881047
AGCGGGA31850.060.0313954
CGGGATA10200.060.06
Produced by FastQC (version 0.11.5)