##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547288_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3358938
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0662697555001	33.0	31.0	34.0	31.0	34.0
2	32.49234787900223	34.0	31.0	34.0	31.0	34.0
3	32.61397054664302	34.0	31.0	34.0	31.0	34.0
4	36.12671356244146	37.0	35.0	37.0	35.0	37.0
5	36.09784044837981	37.0	35.0	37.0	35.0	37.0
6	36.14635756896972	37.0	36.0	37.0	35.0	37.0
7	36.37799953437664	37.0	37.0	37.0	35.0	37.0
8	36.38378469623434	37.0	37.0	37.0	35.0	37.0
9	38.194340592175266	39.0	39.0	39.0	37.0	39.0
10-11	37.724471544279766	39.0	37.5	39.0	35.0	39.0
12-13	37.09997177679374	39.0	35.0	39.0	33.5	39.0
14-15	38.29166421053321	40.0	37.0	41.0	33.5	41.0
16-17	38.43963270533722	40.0	37.0	41.0	34.0	41.0
18-19	38.29742778818782	40.0	36.0	41.0	34.0	41.0
20-21	38.1277356116725	40.0	35.0	41.0	34.0	41.0
22-23	37.94765875404667	40.0	35.0	41.0	34.0	41.0
24-25	37.932913915052914	40.0	35.0	41.0	34.0	41.0
26-27	37.91895801589669	40.0	35.0	41.0	34.0	41.0
28-29	37.83079160734732	40.0	35.0	41.0	34.0	41.0
30-31	37.687081303673956	40.0	35.0	41.0	33.5	41.0
32-33	37.40690926715527	40.0	35.0	41.0	33.0	41.0
34-35	37.11227849397637	40.0	35.0	41.0	32.5	41.0
36-37	36.92561577498602	40.0	35.0	41.0	32.0	41.0
38-39	36.830686663463275	40.0	35.0	41.0	31.0	41.0
40-41	36.73302141927002	40.0	35.0	41.0	31.0	41.0
42-43	36.67447776648453	39.5	35.0	41.0	31.0	41.0
44-45	36.5502565096468	39.0	35.0	41.0	31.0	41.0
46-47	36.434269998434026	39.0	35.0	41.0	31.0	41.0
48-49	36.342350022536884	39.0	35.0	41.0	31.0	41.0
50-51	36.12700502361163	38.5	35.0	40.5	30.5	41.0
52-53	35.946953620459794	38.0	35.0	40.0	30.0	41.0
54-55	35.755655805495664	38.0	35.0	40.0	29.5	41.0
56-57	35.48242599297754	37.0	35.0	40.0	29.0	41.0
58-59	35.24080974403219	37.0	35.0	40.0	28.5	41.0
60-61	34.89909876276371	36.0	34.0	40.0	28.0	41.0
62-63	34.553139414898396	36.0	34.0	39.0	27.0	41.0
64-65	34.21323168215668	35.0	34.0	39.0	26.5	40.0
66-67	33.898995754015104	35.0	34.0	38.5	26.0	40.0
68-69	33.4327107258306	35.0	33.5	37.5	25.5	40.0
70-71	33.14344891153097	35.0	33.0	37.0	25.0	39.0
72-73	32.87083328123353	35.0	33.0	36.5	25.0	39.0
74-75	32.57590226434665	35.0	33.0	36.0	24.5	38.5
76-77	30.621156448853775	33.0	30.0	34.5	20.5	36.0
78-79	31.68641844535386	34.5	32.0	35.0	22.5	37.0
80-81	31.797170712886036	35.0	32.5	35.0	22.0	36.5
82-83	31.612600321887456	35.0	32.0	35.0	20.0	36.0
84-85	31.39521479705788	34.5	32.0	35.0	20.0	36.0
86	30.95062248841747	34.0	31.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	14.0
11	16.0
12	47.0
13	143.0
14	388.0
15	1160.0
16	2711.0
17	6418.0
18	14085.0
19	23215.0
20	27769.0
21	27773.0
22	24512.0
23	21659.0
24	20663.0
25	22586.0
26	26503.0
27	32452.0
28	39657.0
29	49482.0
30	62406.0
31	78354.0
32	98677.0
33	131355.0
34	320480.0
35	307163.0
36	285672.0
37	542088.0
38	816282.0
39	374256.0
40	942.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.525887051204876	23.672273796062925	31.1691373880673	13.632701764664903
2	30.438489784568812	25.059944541995115	31.10253895725375	13.399026716182316
3	29.12036482959793	24.347249041214813	33.43800927555078	13.094376853636478
4	25.792378424371037	26.95027416403637	32.80926888200973	14.448078529582862
5	24.311225750519956	32.49613419479609	29.574347606296993	13.61829244838696
6	22.806226253655172	40.733767637271065	26.22703961787922	10.232966491194539
7	89.29009704853141	2.9994599483527233	6.384071393994173	1.3263716091216926
8	90.28383375936085	2.1477919509082932	6.086953674048166	1.4814206156826948
9	86.03159689163658	5.05150735143072	6.948863003723201	1.9680327532094966
10-11	37.98407413295512	29.6845907843491	20.46865705767716	11.862678025018623
12-13	28.618569321612963	23.88655283306807	29.35905634459463	18.135821500724337
14-15	17.379138882587295	30.287102649706544	34.563573367534616	17.770185100171542
16-17	23.492976053740797	26.821498342630917	31.174332482469165	18.511193121159128
18-19	24.00809422501993	27.637053735436616	30.082156919835974	18.27269511970748
20-21	26.047473933725477	27.545998169659576	29.934491199301682	16.472036697313257
22-23	22.522341883059465	23.550270948734394	33.071092708469166	20.856294459736976
24-25	20.269665590731357	27.396010286584627	33.311957529433414	19.022366593250606
26-27	19.00795727697266	32.08908589560153	29.858291519521945	19.04466530790387
28-29	18.15463697156661	28.123353274159868	34.5737849284506	19.148224825822922
30-31	21.900017207819854	27.558829606262456	31.333311302560514	19.20784188335718
32-33	24.25378199895324	27.71591794787519	29.52373637143645	18.50656368173512
34-35	20.20340953003598	28.27505300782569	30.885491187988585	20.63604627414975
36-37	22.32949819258349	28.852690939814906	29.890057512225592	18.927753355376016
38-39	20.73874242394471	29.634664289724906	29.095803495033252	20.53078979129713
40-41	21.07215137641719	27.607401506071266	28.71502838099423	22.605418736517315
42-43	21.078060982370022	27.072649152797702	29.246952459378527	22.60233740545375
44-45	20.044281853371512	29.66279818204444	28.609712355512368	21.68320760907168
46-47	20.854284895999868	29.169383299126096	28.663509121037663	21.31282268383638
48-49	20.93051434709423	27.23109208922582	30.823924109346468	21.014469454333483
50-51	20.01364717062357	29.453535611553416	29.091635510985913	21.4411817068371
52-53	21.44411418132755	26.873464172306843	30.192117270399155	21.490304375966453
54-55	21.675660580814533	27.456148342124802	30.470404633845575	20.39778644321509
56-57	21.453655887664492	26.63416234536035	29.685856065220612	22.226325701754543
58-59	22.517920842837828	28.34254457807795	28.469980690325336	20.669553888758887
60-61	23.02930271413167	28.526054961419355	27.899621844761647	20.545020479687327
62-63	21.648628227136076	27.076296138839123	30.512769214555313	20.762306419469486
64-65	20.353992839403407	29.621326740773423	29.89004262656828	20.134637793254893
66-67	20.1286537590155	28.81931729612157	27.683511871907136	23.368517072955797
68-69	19.5582800277945	30.29512601899767	27.23464976132337	22.91194419188446
70-71	19.88990270138955	29.75756027649215	28.919721054690502	21.4328159674278
72-73	21.301807297425555	30.38970948555764	27.883977018926814	20.424506198089993
74-75	20.60472685116546	30.746622890925646	27.110041923965255	21.538608333943646
76-77	22.732676220876954	28.581102122158846	27.55161006246617	21.134611594498022
78-79	19.6090847762001	29.567946773652864	29.105226116111698	21.717742334035343
80-81	18.3080485558233	29.477665262056043	29.609343786637325	22.604942395483334
82-83	19.572748886701692	28.736017157804046	29.58417214012286	22.107061815371406
84-85	18.213405546634085	28.660100305513232	29.08356748472285	24.042926663129833
86	19.276658277110208	28.70767486628214	29.56535666928059	22.450310187327066
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	790.0
1	1512.5
2	7307.5
3	8060.0
4	4019.5
5	4948.0
6	6082.5
7	6568.0
8	6981.0
9	7466.0
10	7524.5
11	7642.0
12	7598.5
13	7531.5
14	7639.0
15	7634.5
16	7718.5
17	7675.5
18	8217.0
19	8826.5
20	9466.0
21	10169.0
22	10857.0
23	12484.0
24	13867.0
25	16807.0
26	21808.5
27	26223.5
28	34040.5
29	39948.0
30	44199.0
31	54318.5
32	63419.0
33	70100.5
34	78261.5
35	88306.5
36	93640.0
37	99062.5
38	110861.0
39	121521.0
40	130618.0
41	145077.5
42	159951.5
43	165179.0
44	168433.0
45	170884.0
46	172812.5
47	176243.5
48	169852.0
49	157423.5
50	152086.0
51	139472.5
52	118345.0
53	106809.0
54	102425.5
55	96059.5
56	84823.0
57	78591.0
58	71775.0
59	56624.0
60	41078.0
61	29583.5
62	21615.0
63	14610.0
64	11290.0
65	9419.0
66	6748.0
67	4962.5
68	3224.0
69	2227.0
70	1727.5
71	1472.0
72	1167.5
73	771.0
74	537.5
75	343.5
76	215.5
77	130.0
78	78.0
79	36.0
80	33.0
81	26.5
82	18.0
83	12.0
84	9.5
85	7.0
86	6.0
87	7.5
88	7.0
89	4.0
90	1.5
91	0.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	3358938.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.675455967219
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.2828480758852	13.220776420451374
2	8.490904202570913	2.831793983036921
3	2.6975026288638975	1.3494625892723227
4	1.2602272925215765	0.8405945890052468
5	0.7470119446568098	0.6228382395055634
6	0.5182118424307975	0.5184851256087717
7	0.3673290132568759	0.4287765150232927
8	0.29692024733044786	0.39610244081077267
9	0.2327970640846359	0.3493797472297086
>10	2.8251888016185487	11.640167707978327
>50	1.4425893122683595	17.595520668702047
>100	1.83394578786353	48.88310180904221
>500	0.003076174920871609	0.34190705581166386
>1k	0.0012666602615353684	0.30977123245631816
>5k	0.0	0.0
>10k+	1.8095146593362404E-4	0.671321876065475
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22248	0.6623522077513786	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.954262924769674E-5	0.0	0.0	0.03492175205377414	0.0
2	5.954262924769674E-5	0.0	0.0	0.1106004338275967	0.0
3	5.954262924769674E-5	0.0	0.0	0.16386131568966145	0.0
4	5.954262924769674E-5	0.0	0.0	0.2588615806543616	0.0
5	5.954262924769674E-5	0.0	0.0	0.4824739247940867	0.0
6	5.954262924769674E-5	0.0	0.0	0.6683064706761482	0.0
7	5.954262924769674E-5	0.0	0.0	0.7955490693784761	0.0
8	5.954262924769674E-5	0.0	0.0	1.0458067401065456	0.0
9	5.954262924769674E-5	0.0	0.0	1.1406283771835026	0.0
10	5.954262924769674E-5	0.0	0.0	1.3090744753252368	0.0
11	5.954262924769674E-5	0.0	0.0	1.597856227176566	0.0
12	5.954262924769674E-5	0.0	0.0	1.802801957047138	0.0
13	5.954262924769674E-5	0.0	0.0	1.8850303280382072	0.0
14	5.954262924769674E-5	0.0	0.0	1.9178085454390643	0.0
15	5.954262924769674E-5	0.0	0.0	1.964400652825387	0.0
16	5.954262924769674E-5	0.0	0.0	2.0694338508183243	0.0
17	5.954262924769674E-5	0.0	0.0	2.2188263076007955	0.0
18	5.954262924769674E-5	0.0	0.0	2.388760971473722	0.0
19	5.954262924769674E-5	0.0	0.0	2.515140202051958	0.0
20	5.954262924769674E-5	0.0	0.0	2.646878269262487	0.0
21	5.954262924769674E-5	0.0	0.0	2.802850186576829	0.0
22	5.954262924769674E-5	0.0	0.0	2.9869559962107073	0.0
23	5.954262924769674E-5	0.0	0.0	3.169037356450164	0.0
24	5.954262924769674E-5	0.0	0.0	3.3093197909577374	0.0
25	5.954262924769674E-5	0.0	0.0	3.4298340725550753	0.0
26	5.954262924769674E-5	0.0	0.0	3.5485620752749827	0.0
27	5.954262924769674E-5	0.0	0.0	3.683396359206392	0.0
28	5.954262924769674E-5	0.0	0.0	3.819064239947269	0.0
29	8.931394387154511E-5	0.0	0.0	3.9852179468629667	0.0
30	8.931394387154511E-5	0.0	0.0	4.167626791563285	0.0
31	8.931394387154511E-5	0.0	0.0	4.345540167755404	0.0
32	8.931394387154511E-5	0.0	0.0	4.506900693016662	0.0
33	8.931394387154511E-5	0.0	0.0	4.661324501970563	0.0
34	8.931394387154511E-5	0.0	0.0	4.827269809683894	0.0
35	8.931394387154511E-5	0.0	0.0	5.019919986614817	0.0
36	8.931394387154511E-5	0.0	0.0	5.200036440089099	0.0
37	8.931394387154511E-5	0.0	0.0	5.3861666991174	0.0
38	8.931394387154511E-5	0.0	0.0	5.604271350051713	0.0
39	8.931394387154511E-5	0.0	0.0	5.910618177531113	0.0
40	8.931394387154511E-5	0.0	0.0	6.1382198778304335	0.0
41	1.1908525849539348E-4	0.0	0.0	6.354657335145811	0.0
42	1.1908525849539348E-4	0.0	0.0	6.562580196478768	0.0
43	1.1908525849539348E-4	0.0	0.0	6.766781643483744	0.0
44	1.1908525849539348E-4	0.0	0.0	6.959640219617033	0.0
45	1.1908525849539348E-4	0.0	0.0	7.159227112855313	0.0
46	1.1908525849539348E-4	0.0	0.0	7.3844173366701025	0.0
47	1.1908525849539348E-4	0.0	0.0	7.616722904679991	0.0
48	1.1908525849539348E-4	0.0	0.0	7.83455961378269	0.0
49	1.1908525849539348E-4	0.0	0.0	8.05349786152647	0.0
50	1.1908525849539348E-4	0.0	0.0	8.286249999255718	0.0
51	1.4885657311924184E-4	0.0	0.0	8.51974641985056	0.0
52	1.4885657311924184E-4	0.0	0.0	8.793195944670607	0.0
53	1.4885657311924184E-4	0.0	0.0	9.038303177968752	0.0
54	1.4885657311924184E-4	0.0	0.0	9.278706543556327	0.0
55	1.4885657311924184E-4	0.0	0.0	9.520925959335957	0.0
56	1.4885657311924184E-4	0.0	0.0	9.76826604123089	0.0
57	1.7862788774309022E-4	0.0	0.0	10.020369533465637	0.0
58	1.7862788774309022E-4	2.977131462384837E-5	0.0	10.265953107797761	0.0
59	1.7862788774309022E-4	2.977131462384837E-5	0.0	10.527434564139023	0.0
60	1.7862788774309022E-4	2.977131462384837E-5	0.0	10.802908538353492	0.0
61	1.7862788774309022E-4	2.977131462384837E-5	0.0	11.074303842464493	0.0
62	1.7862788774309022E-4	2.977131462384837E-5	0.0	11.383657572720901	0.0
63	1.7862788774309022E-4	2.977131462384837E-5	0.0	11.696941116507658	0.0
64	1.7862788774309022E-4	2.977131462384837E-5	0.0	11.996232142421206	0.0
65	1.7862788774309022E-4	2.977131462384837E-5	0.0	12.28242378990026	0.0
66	1.7862788774309022E-4	2.977131462384837E-5	0.0	12.547567117940254	0.0
67	1.7862788774309022E-4	2.977131462384837E-5	0.0	12.824678514459034	0.0
68	1.7862788774309022E-4	2.977131462384837E-5	0.0	13.140581933932689	0.0
69	1.7862788774309022E-4	2.977131462384837E-5	0.0	13.563007117130473	0.0
70	1.7862788774309022E-4	2.977131462384837E-5	0.0	13.867180638642333	0.0
71	1.7862788774309022E-4	2.977131462384837E-5	0.0	14.162154823935422	0.0
72	1.7862788774309022E-4	2.977131462384837E-5	0.0	14.453526680159026	0.0
73	1.7862788774309022E-4	2.977131462384837E-5	0.0	14.739896955525824	0.0
74	1.7862788774309022E-4	2.977131462384837E-5	0.0	15.075657841853586	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	12315	0.0	72.756805	1
TACGGGA	1710	0.0	69.94152	4
CGTTAGG	710	0.0	68.169014	2
ACGGGAT	1770	0.0	64.858765	5
GTAGGGA	5275	0.0	64.75829	4
GCGGGAT	3020	0.0	64.50331	5
GGCGATA	530	0.0	62.641514	8
TAGGGAT	6095	0.0	62.47744	5
TAGGGAC	4655	0.0	62.126747	5
TCGTTAG	200	0.0	62.0	1
GTACGGG	1395	0.0	61.935486	3
AGGGATT	7030	0.0	61.90612	6
CGGGATT	1760	0.0	61.590908	6
TACGCGG	260	0.0	61.538464	2
CGTAAGG	615	0.0	61.13821	2
CGGGAAT	845	0.0	61.06509	6
TTGCGGG	2160	0.0	60.370373	3
GGGCGAT	2270	0.0	60.088104	7
AGCGGGA	3185	0.0	60.031395	4
CGGGATA	1020	0.0	60.0	6
>>END_MODULE