FastQCFastQC Report
Thu 26 May 2016
SRR1547287_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547287_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1626919
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105870.6507392193465071No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACG404.1836756E-1180.01
TAACGCG204.091412E-580.01
TACGCGG850.080.02
AGTACGG1250.073.62
CGTTTTT61950.071.928971
TAAGGGA17750.066.4788744
TAGCGGG10450.065.837323
TTAGCGG2500.065.62
ATATGCG1650.065.4545441
TAGGGTA9600.064.1666645
ACGTTAG750.064.01
CGTTAGG3500.064.02
GTAAGGG22850.063.019693
AGGGACC13650.062.710626
TTGCGGG11200.062.1428573
GGGCGAT9850.062.1319777
TAACGGG4950.061.4141433
CGTAAGG1900.061.052632
GTACGAG1050.060.9523851
TAGTAGG10650.060.8450742
Produced by FastQC (version 0.11.5)