##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547287_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1626919
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02383585169268	33.0	31.0	34.0	31.0	34.0
2	32.435617261830494	34.0	31.0	34.0	31.0	34.0
3	32.54031147217532	34.0	31.0	34.0	31.0	34.0
4	36.0803654023341	37.0	35.0	37.0	35.0	37.0
5	36.05164916016101	37.0	35.0	37.0	35.0	37.0
6	36.10876140729809	37.0	35.0	37.0	35.0	37.0
7	36.338007608246016	37.0	37.0	37.0	35.0	37.0
8	36.327895242479805	37.0	37.0	37.0	35.0	37.0
9	38.0956513508048	39.0	39.0	39.0	37.0	39.0
10-11	37.6604529789129	39.0	37.5	39.0	35.0	39.0
12-13	36.887027258271615	39.0	35.0	39.0	33.5	39.0
14-15	37.959005949282044	40.0	35.0	41.0	33.0	41.0
16-17	38.12675031762491	40.0	35.0	41.0	34.0	41.0
18-19	37.9862845046373	40.0	36.0	41.0	34.0	41.0
20-21	37.815836867108935	40.0	35.0	41.0	34.0	41.0
22-23	37.629415170638495	39.5	35.0	41.0	34.0	41.0
24-25	37.58832492582606	39.0	35.0	41.0	34.0	41.0
26-27	37.573280230915	39.0	35.0	41.0	34.0	41.0
28-29	37.494918616108116	39.0	35.0	41.0	33.5	41.0
30-31	37.32719084355152	39.0	35.0	41.0	33.0	41.0
32-33	36.96968564507514	39.0	35.0	41.0	32.5	41.0
34-35	36.623350025416144	39.0	35.0	41.0	31.5	41.0
36-37	36.424235318414745	39.0	35.0	41.0	31.0	41.0
38-39	36.33104444658892	39.0	35.0	41.0	31.0	41.0
40-41	36.202487032237	39.0	35.0	41.0	30.0	41.0
42-43	36.17938600508077	39.0	35.0	41.0	30.5	41.0
44-45	36.02656708785133	38.0	35.0	41.0	30.0	41.0
46-47	35.87865622074609	38.0	35.0	40.5	30.0	41.0
48-49	35.79256619413751	38.0	35.0	40.0	30.0	41.0
50-51	35.546585294043524	37.5	35.0	40.0	29.0	41.0
52-53	35.33823195869002	37.0	35.0	40.0	28.0	41.0
54-55	35.12119226587187	36.5	35.0	40.0	28.0	41.0
56-57	34.81064853259443	36.0	34.0	40.0	27.5	41.0
58-59	34.53979669547162	36.0	34.0	39.0	26.5	41.0
60-61	34.13024926256317	35.0	34.0	39.0	26.0	40.5
62-63	33.69537235719787	35.0	33.5	38.5	25.5	40.0
64-65	33.28559780788103	35.0	33.0	38.0	24.0	40.0
66-67	32.93236510238063	35.0	33.0	37.0	22.5	40.0
68-69	32.43637667271696	35.0	32.5	36.5	20.5	39.5
70-71	32.09098824219276	35.0	32.0	36.0	19.5	39.0
72-73	31.808174223793564	35.0	32.0	36.0	18.0	38.5
74-75	31.494930909283127	35.0	32.0	35.0	17.5	37.5
76-77	29.591490418392066	33.0	29.0	34.5	13.5	35.5
78-79	30.538663879394118	34.0	30.5	35.0	15.0	36.0
80-81	30.640831227614896	34.0	31.0	35.0	11.5	36.0
82-83	30.400553131409737	34.0	31.0	35.0	10.0	36.0
84-85	30.138495831691685	34.0	31.0	35.0	10.0	35.5
86	29.772529548182792	34.0	30.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	6.0
10	7.0
11	13.0
12	21.0
13	55.0
14	202.0
15	721.0
16	1693.0
17	4203.0
18	9083.0
19	14328.0
20	16101.0
21	15282.0
22	12746.0
23	11425.0
24	11364.0
25	12697.0
26	15308.0
27	18549.0
28	23271.0
29	30023.0
30	38873.0
31	49102.0
32	58568.0
33	73965.0
34	192313.0
35	173047.0
36	139596.0
37	243537.0
38	328671.0
39	131901.0
40	245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.04840683525117	22.98749968498739	29.3211278496348	13.642965630126639
2	32.41157058218633	23.989454914473306	30.292104278086367	13.306870225253991
3	29.54406457850698	23.77450874935999	33.028319172620144	13.653107499512885
4	26.016230678970498	26.40346569189984	33.08781813968612	14.492485489443544
5	24.860426364189	30.345456657645524	30.974191093717636	13.819925884447843
6	21.970116520859367	40.10722107246888	27.615326884743496	10.307335521928259
7	85.93722244315789	3.943527612622386	8.033282542031902	2.0859674021878165
8	86.03648983139296	3.6446805280410395	7.8861947029938175	2.432634937572184
9	81.32138108904007	6.064469097723981	9.35221728924427	3.2619325239916677
10-11	41.34320762127678	24.713430723963516	20.66147730772091	13.281884347038789
12-13	30.05512259676112	25.24686232074246	27.90458529281421	16.793429789682214
14-15	17.904763543851907	30.649466875732596	35.1046671653598	16.341102415055698
16-17	22.502165135449275	24.862915732129256	32.689888064494916	19.945031067926553
18-19	24.00946205680799	27.216812883739138	30.320624444117993	18.453100615334876
20-21	26.174935568396464	27.693480744892646	29.042503037950873	17.089080648760017
22-23	21.525964107616915	25.103800496521337	31.076623974518707	22.29361142134304
24-25	20.587718257639132	26.12594726596714	33.62957836253679	19.65675611385693
26-27	18.78910382139492	32.14659119476753	29.41440231505072	19.64990266878683
28-29	17.952245932342052	28.416596032131903	33.42984500150284	20.201313034023205
30-31	23.104377046429477	28.116980624112202	29.563180465653176	19.215461863805146
32-33	25.885369830950406	27.510158772501885	28.103949858597755	18.500521537949954
34-35	20.35559238044426	28.524622307564172	29.71779787438711	21.401987437604454
36-37	23.621120658127417	29.94073460325929	28.05726652648349	18.380878212129797
38-39	21.06257287547813	30.790100797888524	27.240477245640378	20.90684908099297
40-41	21.328689381585683	26.77305999868463	28.40079930223939	23.4974513174903
42-43	21.525441647678832	26.66346634343812	27.98055711439844	23.830534894484607
44-45	20.06885407325134	30.662651305934713	27.238541070575735	22.02995355023821
46-47	21.371008636570103	29.38409963864212	27.747539982015084	21.49735174277269
48-49	21.980442787870818	25.83653519320876	30.824798284364498	21.35822373455593
50-51	19.45797547388653	29.75461593355293	28.458485025990843	22.328923566569696
52-53	21.82090810913143	26.27199018512907	30.31229581804626	21.59480588769324
54-55	22.064128576776103	26.608761714627466	30.269638500748965	21.057471207847474
56-57	22.733707086830997	25.63465052654742	28.62345943467376	23.008182951947823
58-59	23.40048889957029	28.437801759030414	27.12907034707936	21.03263899431994
60-61	24.353609491314565	29.163191283647187	26.175734624772346	20.307464600265902
62-63	22.68653202771619	26.85487107840034	29.304532063366402	21.15406483051707
64-65	20.528372955260835	30.298250865593186	28.761050796013816	20.412325383132167
66-67	19.674366087063955	29.60792762270279	26.395628792828653	24.322077497404603
68-69	19.045785315679513	31.106342725114157	26.340186573517183	23.507685385689143
70-71	19.81039006858977	30.17654843295825	28.141105980076453	21.87195551837553
72-73	21.906437874288763	30.853656512708994	26.416404258601688	20.823501354400555
74-75	20.934723855336376	31.881611807348737	25.40292417754049	21.780740159774396
76-77	24.04016426140453	29.012630622667757	25.69504074880188	21.252164367125836
78-79	19.544181363669612	30.139576709104755	28.43076391633511	21.885478010890523
80-81	17.65011042344456	29.768998948318874	29.183813084732552	23.39707754350401
82-83	19.20353748404192	29.141524562685667	29.53690380406154	22.11803414921087
84-85	17.66483150052338	28.84621176592074	28.14952680496079	25.33942992859509
86	18.953678701889892	28.774696220278944	29.6885708507922	22.58305422703896
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	458.0
1	695.5
2	3421.5
3	3790.5
4	1956.5
5	2702.0
6	3563.0
7	3964.0
8	4183.5
9	4207.5
10	3861.5
11	3663.0
12	3623.5
13	3569.0
14	3506.0
15	3658.5
16	3939.5
17	4199.5
18	4582.5
19	5061.5
20	5264.5
21	5240.0
22	5635.5
23	6557.0
24	7417.0
25	8317.0
26	10042.0
27	11768.0
28	13257.0
29	14179.0
30	16597.0
31	19331.0
32	21385.0
33	24603.5
34	30037.5
35	33878.5
36	33766.0
37	36597.0
38	43424.0
39	49928.5
40	59059.0
41	71618.5
42	81118.5
43	84681.0
44	87350.5
45	88533.5
46	90662.0
47	93567.0
48	95326.0
49	88748.5
50	79702.0
51	72068.0
52	60105.5
53	53454.5
54	49170.0
55	46808.0
56	43226.5
57	40045.0
58	36467.5
59	27751.0
60	19459.0
61	15310.0
62	12629.5
63	9134.5
64	7324.0
65	6263.5
66	4319.5
67	3114.0
68	2178.0
69	1513.0
70	1085.0
71	708.0
72	583.0
73	411.0
74	403.5
75	272.5
76	84.0
77	50.5
78	38.5
79	42.0
80	27.0
81	16.5
82	16.0
83	9.0
84	5.0
85	4.0
86	5.0
87	4.5
88	2.0
89	1.0
90	1.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1626919.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.897052780856274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.17852228858784	17.900577511711443
2	9.174823412462471	4.201528318603782
3	3.1898153445991633	2.1911211091941687
4	1.4857799056280272	1.3607992367960235
5	0.9573025395113075	1.095970338721908
6	0.6242227434079708	0.8575716661693927
7	0.4548769801438868	0.7290739556205762
8	0.36807512931352915	0.67422685305699
9	0.2837844450767642	0.584804467557781
>10	3.232108326688669	17.111013000099227
>50	1.0566960805060717	17.356313941471566
>100	0.9896687461993228	34.56152844102197
>500	0.0029727897890533513	0.4269727822488925
>1k	0.0010810144687466734	0.2933736174843477
>5k	0.0	0.0
>10k+	2.7025361718666834E-4	0.6551247602418877
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10587	0.6507392193465071	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07726260496066491	0.0
2	0.0	0.0	0.0	0.23670508488744677	0.0
3	0.0	0.0	0.0	0.34427036625670976	0.0
4	0.0	0.0	0.0	0.543112472102176	0.0
5	0.0	0.0	0.0	1.0144328021247524	0.0
6	0.0	0.0	0.0	1.3869774709128113	0.0
7	0.0	0.0	0.0	1.6407700690692038	0.0
8	0.0	0.0	0.0	2.1814239061686536	0.0
9	0.0	0.0	0.0	2.337301365341483	0.0
10	0.0	0.0	0.0	2.715992621636357	0.0
11	0.0	0.0	0.0	3.295984618779423	0.0
12	0.0	0.0	0.0	3.718992770998433	0.0
13	0.0	0.0	0.0	3.8822461351794404	0.0
14	0.0	0.0	0.0	3.938610342616934	0.0
15	0.0	0.0	0.0	4.038615321352815	0.0
16	0.0	0.0	0.0	4.276488257866556	0.0
17	0.0	0.0	0.0	4.590210084214395	0.0
18	0.0	0.0	0.0	4.955010052743868	0.0
19	0.0	0.0	0.0	5.190117024879542	0.0
20	0.0	0.0	0.0	5.426699178016853	0.0
21	0.0	0.0	0.0	5.756463597757479	0.0
22	0.0	0.0	0.0	6.118743465409157	0.0
23	0.0	0.0	0.0	6.541014027127349	0.0
24	0.0	0.0	0.0	6.826707414444112	0.0
25	0.0	0.0	0.0	7.078717502223528	0.0
26	0.0	0.0	0.0	7.3164060411120655	0.0
27	0.0	0.0	0.0	7.561839280259189	0.0
28	0.0	0.0	0.0	7.836591741813821	0.0
29	0.0	0.0	0.0	8.142200072652665	0.0
30	0.0	0.0	0.0	8.459425453879389	0.0
31	0.0	0.0	0.0	8.76079263933853	0.0
32	0.0	0.0	0.0	9.052140887161562	0.0
33	0.0	0.0	0.0	9.339493853105164	0.0
34	0.0	0.0	0.0	9.625986296797812	0.0
35	0.0	0.0	0.0	9.944686859026172	0.0
36	0.0	0.0	0.0	10.250848382740628	0.0
37	0.0	0.0	0.0	10.567766434592011	0.0
38	0.0	0.0	0.0	10.9277105989911	0.0
39	0.0	0.0	0.0	11.372231807483962	0.0
40	0.0	0.0	0.0	11.709802393358244	0.0
41	0.0	0.0	0.0	12.044176753728982	0.0
42	0.0	0.0	0.0	12.370560550340858	0.0
43	0.0	0.0	0.0	12.680164163059132	0.0
44	0.0	0.0	0.0	12.97765899838898	0.0
45	0.0	0.0	0.0	13.290151507235455	0.0
46	0.0	0.0	0.0	13.627906490734942	0.0
47	0.0	0.0	0.0	13.961973521730338	0.0
48	0.0	0.0	0.0	14.293274588347668	0.0
49	0.0	0.0	0.0	14.629431459095382	0.0
50	0.0	0.0	0.0	14.975976062729613	0.0
51	0.0	0.0	0.0	15.325163698991775	0.0
52	0.0	0.0	0.0	15.714058290547962	0.0
53	0.0	0.0	0.0	16.062139541058897	0.0
54	6.146587506815029E-5	0.0	0.0	16.402783420686585	0.0
55	6.146587506815029E-5	0.0	0.0	16.738387098558686	0.0
56	6.146587506815029E-5	0.0	0.0	17.0855463609436	0.0
57	6.146587506815029E-5	0.0	0.0	17.430185522450717	0.0
58	6.146587506815029E-5	0.0	0.0	17.763330565320093	0.0
59	6.146587506815029E-5	0.0	0.0	18.105941352949962	0.0
60	6.146587506815029E-5	0.0	0.0	18.467053368975346	0.0
61	6.146587506815029E-5	0.0	0.0	18.841257616390244	0.0
62	6.146587506815029E-5	0.0	0.0	19.233778694575452	0.0
63	6.146587506815029E-5	0.0	0.0	19.65223837203942	0.0
64	6.146587506815029E-5	0.0	0.0	20.012612797563985	0.0
65	6.146587506815029E-5	0.0	0.0	20.355838243944536	0.0
66	6.146587506815029E-5	0.0	0.0	20.692240978192523	0.0
67	6.146587506815029E-5	0.0	0.0	21.037925059575798	0.0
68	6.146587506815029E-5	0.0	0.0	21.436469793517684	0.0
69	6.146587506815029E-5	0.0	0.0	21.94178075245295	0.0
70	6.146587506815029E-5	0.0	0.0	22.307072447982968	0.0
71	1.2293175013630058E-4	0.0	0.0	22.65521516436897	0.0
72	1.2293175013630058E-4	0.0	0.0	23.002312960878815	0.0
73	1.2293175013630058E-4	0.0	0.0	23.351131801890567	0.0
74	1.8439762520445086E-4	0.0	0.0	23.731789966187623	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACG	40	4.1836756E-11	80.0	1
TAACGCG	20	4.091412E-5	80.0	1
TACGCGG	85	0.0	80.0	2
AGTACGG	125	0.0	73.6	2
CGTTTTT	6195	0.0	71.92897	1
TAAGGGA	1775	0.0	66.478874	4
TAGCGGG	1045	0.0	65.83732	3
TTAGCGG	250	0.0	65.6	2
ATATGCG	165	0.0	65.454544	1
TAGGGTA	960	0.0	64.166664	5
ACGTTAG	75	0.0	64.0	1
CGTTAGG	350	0.0	64.0	2
GTAAGGG	2285	0.0	63.01969	3
AGGGACC	1365	0.0	62.71062	6
TTGCGGG	1120	0.0	62.142857	3
GGGCGAT	985	0.0	62.131977	7
TAACGGG	495	0.0	61.414143	3
CGTAAGG	190	0.0	61.05263	2
GTACGAG	105	0.0	60.952385	1
TAGTAGG	1065	0.0	60.845074	2
>>END_MODULE