FastQCFastQC Report
Thu 26 May 2016
SRR1547286_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547286_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1758761
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163110.9274142421852656No Hit
ACGCCGGGGGAGATTTTGTGTACCCTTCTTGCCTCCATAAAAACCATCAC39700.22572708855836582No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG38690.2199844094791731TruSeq Adapter, Index 23 (95% over 23bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA30720.1746684171413853No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGC28250.16062443959128045No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC22590.1284426934643195Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACCG204.091505E-580.01
CGAAACG2600.076.9230880
CGTTTTT87050.074.899481
GGCGATT5700.071.578948
TGTTGCG1650.070.3030241
TAGGGTA10350.069.951695
CGCCGGG7400.069.1891862
GATTGCG1100.069.0909041
CGGGTAT2500.068.86
TAGGGCG3900.068.717955
GTTAGCG351.181852E-768.571431
AGTACGG2750.068.363642
GTAAGCG2300.067.826091
ACGTTAG650.067.692311
AATGCGG1950.067.692312
AAGTGCG650.067.692311
CGGGTAC1200.066.6666646
GTGCGCG303.7964219E-666.6666641
AACGTTG1100.065.4545441
ACGGGAT4950.065.4545445
Produced by FastQC (version 0.11.5)