##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547286_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1758761
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91008215442576	31.0	31.0	34.0	30.0	34.0
2	32.31856119165708	33.0	31.0	34.0	31.0	34.0
3	32.39922536376461	34.0	31.0	34.0	30.0	34.0
4	35.96169462479553	37.0	35.0	37.0	35.0	37.0
5	35.91994023065101	37.0	35.0	37.0	35.0	37.0
6	35.9790807278533	37.0	35.0	37.0	35.0	37.0
7	36.27488100998373	37.0	36.0	37.0	35.0	37.0
8	36.267474659717834	37.0	37.0	37.0	35.0	37.0
9	38.03450099246003	39.0	39.0	39.0	35.0	39.0
10-11	37.512451379124286	39.0	37.0	39.0	35.0	39.0
12-13	36.51960954330918	39.0	35.0	39.0	33.0	39.0
14-15	37.54806252810928	40.0	35.0	41.0	33.0	41.0
16-17	37.77357753554918	40.0	35.0	41.0	34.0	41.0
18-19	37.65954441791693	39.0	35.5	41.0	34.0	41.0
20-21	37.40258881110054	39.0	35.0	41.0	33.5	41.0
22-23	37.19524853007316	39.0	35.0	41.0	33.0	41.0
24-25	37.14083806725303	38.0	35.0	41.0	33.0	41.0
26-27	37.07759468171059	38.0	35.0	41.0	33.0	41.0
28-29	37.02267874941507	38.0	35.0	41.0	33.0	41.0
30-31	36.85374590407679	38.0	35.0	41.0	33.0	41.0
32-33	36.462195261323174	38.0	35.0	41.0	31.5	41.0
34-35	36.11372352468584	38.0	35.0	41.0	31.0	41.0
36-37	35.880741897278824	38.0	35.0	41.0	30.0	41.0
38-39	35.80517477929065	38.0	35.0	41.0	30.0	41.0
40-41	35.652487745634566	38.0	35.0	40.0	29.5	41.0
42-43	35.60461626110654	38.0	35.0	40.0	30.0	41.0
44-45	35.454874482661374	37.0	35.0	40.0	29.0	41.0
46-47	35.29576957869773	37.0	35.0	40.0	29.0	41.0
48-49	35.222278922491455	37.0	35.0	40.0	28.0	41.0
50-51	35.001614488836175	36.0	35.0	40.0	27.5	41.0
52-53	34.795317555938524	36.0	34.0	40.0	27.0	41.0
54-55	34.619406218354854	36.0	34.0	40.0	26.0	41.0
56-57	34.327305415573804	35.5	34.0	40.0	25.5	41.0
58-59	33.99604693303979	35.0	34.0	39.0	24.5	41.0
60-61	33.568005260521474	35.0	33.5	39.0	22.5	40.5
62-63	32.94350056659205	35.0	32.5	38.0	21.0	40.0
64-65	32.31249385220619	35.0	32.0	37.5	18.0	40.0
66-67	31.809686478151377	35.0	32.0	37.0	14.0	39.5
68-69	31.21056328858782	35.0	31.0	36.0	11.0	39.0
70-71	30.65454572849864	35.0	30.0	36.0	10.5	39.0
72-73	30.250262542778692	35.0	30.0	35.0	10.0	37.5
74-75	29.8871591990043	34.5	29.0	35.0	8.0	37.0
76-77	28.02105402610133	32.0	25.5	34.5	8.0	35.5
78-79	28.755358175442826	33.5	26.5	35.0	7.5	36.0
80-81	28.872205205823875	34.0	27.0	35.0	7.0	36.0
82-83	28.565013097288375	34.0	25.5	35.0	7.0	36.0
84-85	28.264990808870564	34.0	25.0	35.0	7.0	35.0
86	27.876706954498083	34.0	24.0	35.0	7.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	7.0
10	5.0
11	7.0
12	26.0
13	46.0
14	274.0
15	867.0
16	2319.0
17	5805.0
18	12708.0
19	18432.0
20	19156.0
21	18400.0
22	15231.0
23	14048.0
24	14891.0
25	17910.0
26	21760.0
27	27465.0
28	34738.0
29	44649.0
30	57794.0
31	71232.0
32	81624.0
33	95633.0
34	231373.0
35	225268.0
36	131598.0
37	199414.0
38	274680.0
39	121161.0
40	235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.077629080926858	25.671310655626318	31.14425439272306	14.106805870723765
2	30.908406543015225	25.581986409750957	30.407826873577477	13.10178017365634
3	27.54711981900895	23.890568417198242	35.01999418909107	13.542317574701737
4	24.649511786990956	26.832412135588633	34.41155449774017	14.106521579680239
5	23.83513166371099	30.101474845075593	32.51459408071932	13.548799410494091
6	21.55398033047128	38.690873859495404	28.60127100839739	11.153874801635924
7	84.38184608369187	3.648534394383319	9.60346516667131	2.3661543552534994
8	84.27552123341376	3.356794925518589	9.2210368549223	3.14664698614536
9	80.24131760938525	4.986351187000395	11.01104698136927	3.761284222245092
10-11	44.628775598276285	22.19741624928003	20.091558773477466	13.082249378966216
12-13	28.937047159904044	29.319589188070466	26.80494962078418	14.938414031241312
14-15	14.268624332697849	34.91759255521358	36.36616913838776	14.447613973700804
16-17	15.123402213262633	25.46423305952315	36.40022720540198	23.01213752181223
18-19	21.494591931479036	28.427682897221395	30.61041267119296	19.46731250010661
20-21	23.54259617992439	29.356433307311224	29.01008152898546	18.09088898377892
22-23	18.325798673043124	29.144863912720375	27.715931840653734	24.813405573582767
24-25	16.860079339944427	28.45793146425239	35.094734304433636	19.58725489136955
26-27	15.730761598648138	36.00833200190362	30.026450438689512	18.234455960758737
28-29	14.524571559182856	28.35510339380962	37.64735515513478	19.472969891872744
30-31	20.363966451382538	30.504997552254114	30.779253121942098	18.351782874421254
32-33	23.479341422740212	28.93363566738175	28.252701759932137	19.3343211499459
34-35	17.24128520020628	29.16931294246347	30.646688208346674	22.942713648983574
36-37	21.433355640703883	28.936023712147357	30.61390945102831	19.01671119612045
38-39	18.033462192986995	31.80633980398701	28.339040949850492	21.8211570531755
40-41	19.24820939286236	27.981715537244682	28.938809764373897	23.831265305519057
42-43	21.094679720553273	26.128308508091774	28.039511906393194	24.737499864961755
44-45	17.035373197381563	33.52249111732635	26.895382601729285	22.546753083562805
46-47	20.40248788778009	30.794860700231585	27.695320739998213	21.10733067199011
48-49	19.778696480078874	25.238847120217017	32.49958351362124	22.482872886082873
50-51	17.301952908894386	30.921370214599936	28.593765724848346	23.182911151657333
52-53	21.232447160245197	25.722198752417185	31.537229902186827	21.508124185150795
54-55	20.674440700015523	26.7207141845879	31.87161302758021	20.73323208781637
56-57	21.85001259409323	24.059693158990903	30.59653926826897	23.4937549786469
58-59	22.52594297917682	28.301542961209623	28.639508153751418	20.53300590586214
60-61	22.74589895955164	31.668629222503796	25.806206755778643	19.779265062165923
62-63	20.893287945320598	28.781340955365735	30.473640250153377	19.85173084916029
64-65	16.46275417751474	35.100050546947536	29.290420927004863	19.14677434853286
66-67	15.19925106367494	32.35561852918048	25.128030471451208	27.317099935693367
68-69	15.638139576667893	35.69882434281861	24.035329416560863	24.627706663952633
70-71	16.986190846851844	33.115727492251644	27.576686087535485	22.32139557336102
72-73	20.01050739696866	33.277460666912674	26.211776358470534	20.500255577648126
74-75	19.715782872146924	35.07292918139531	23.421772486426523	21.78951546003124
76-77	24.674898977177683	31.190082108939194	24.915608203729786	19.21941071015334
78-79	16.989687626687196	32.753625990114635	29.70460454831555	20.552081834882625
80-81	14.258787862591904	31.74709355051653	30.753809073546662	23.240309513344908
82-83	16.446009435051153	29.695620951340175	31.25916483251562	22.599204781093054
84-85	13.90521509176062	30.306732978500207	29.278907139742127	26.50914478999705
86	16.119870749919972	29.361180967738083	31.44440887647611	23.074539405865835
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1114.0
1	1303.0
2	5566.5
3	5983.0
4	2830.5
5	4775.0
6	7738.0
7	9262.0
8	9648.0
9	9446.0
10	7788.5
11	6116.5
12	5358.5
13	5271.0
14	5339.0
15	5424.0
16	5510.0
17	5728.0
18	6280.5
19	6525.0
20	6620.0
21	6806.0
22	6857.5
23	6976.0
24	7535.5
25	8990.5
26	10334.5
27	11601.5
28	14958.0
29	17429.0
30	19044.0
31	21283.0
32	22604.5
33	26918.5
34	31262.0
35	34987.5
36	37986.0
37	42727.0
38	50922.0
39	60753.5
40	75539.0
41	91288.0
42	106669.5
43	114710.0
44	119819.5
45	123071.5
46	120654.0
47	118053.5
48	107737.5
49	84817.5
50	70173.0
51	62640.0
52	49831.5
53	39887.0
54	32817.5
55	30894.5
56	29428.5
57	27485.0
58	25859.0
59	18721.0
60	10747.5
61	7320.0
62	5436.5
63	4120.0
64	3721.0
65	2981.0
66	1594.0
67	811.5
68	563.0
69	408.5
70	311.5
71	256.0
72	220.0
73	125.0
74	46.0
75	30.5
76	33.0
77	19.5
78	14.5
79	21.0
80	14.0
81	3.5
82	2.0
83	3.0
84	1.0
85	0.5
86	1.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1758761.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.185910828251373
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.28947689044341	17.45655097487513
2	10.688664547062398	4.956528461225612
3	4.046061777776842	2.814348827553902
4	2.0012449413800804	1.8560274702531077
5	1.2278860902461644	1.423482869784889
6	0.8688508636316838	1.2087059188328049
7	0.6270570213090649	1.017722172621063
8	0.4629097718660837	0.8586387753610562
9	0.3640809090513818	0.7597392742380632
>10	2.8051246718019387	13.598893194323605
>50	0.6514609363825287	10.881521689215928
>100	0.9558348938677839	39.55330554864604
>500	0.007646679143429103	1.1199910670911084
>1k	0.00345333896800024	1.5616849347898587
>5k	0.0	0.0
>10k+	2.4666706914287426E-4	0.9328588211879216
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16311	0.9274142421852656	No Hit
ACGCCGGGGGAGATTTTGTGTACCCTTCTTGCCTCCATAAAAACCATCAC	3970	0.22572708855836582	No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG	3869	0.2199844094791731	TruSeq Adapter, Index 23 (95% over 23bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3072	0.1746684171413853	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGC	2825	0.16062443959128045	No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	2259	0.1284426934643195	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.6858208704878036E-5	0.0	0.0	0.13407165612610242	0.0
2	5.6858208704878036E-5	0.0	0.0	0.6319789897547193	0.0
3	5.6858208704878036E-5	0.0	0.0	0.9992830179882315	0.0
4	5.6858208704878036E-5	0.0	0.0	1.5455198290159948	0.0
5	5.6858208704878036E-5	0.0	0.0	2.817096808491887	0.0
6	5.6858208704878036E-5	0.0	0.0	3.8172895578193966	0.0
7	5.6858208704878036E-5	0.0	0.0	4.450576286374329	0.0
8	5.6858208704878036E-5	0.0	0.0	5.604627348457238	0.0
9	5.6858208704878036E-5	0.0	0.0	5.989784854224082	0.0
10	5.6858208704878036E-5	0.0	0.0	6.787278089518701	0.0
11	5.6858208704878036E-5	0.0	0.0	8.021328651249373	0.0
12	5.6858208704878036E-5	0.0	0.0	8.86880025199558	0.0
13	5.6858208704878036E-5	0.0	0.0	9.196132959509564	0.0
14	5.6858208704878036E-5	0.0	0.0	9.314113742572186	0.0
15	5.6858208704878036E-5	0.0	0.0	9.513174331247964	0.0
16	5.6858208704878036E-5	0.0	0.0	9.998800291796327	0.0
17	5.6858208704878036E-5	0.0	0.0	10.575399386272496	0.0
18	5.6858208704878036E-5	0.0	0.0	11.280384316004278	0.0
19	5.6858208704878036E-5	0.0	0.0	11.694141500749675	0.0
20	5.6858208704878036E-5	0.0	0.0	12.074124909524375	0.0
21	5.6858208704878036E-5	0.0	0.0	12.60102992959248	0.0
22	5.6858208704878036E-5	0.0	0.0	13.132369889939566	0.0
23	5.6858208704878036E-5	0.0	0.0	13.762586275224434	0.0
24	5.6858208704878036E-5	0.0	0.0	14.181119549501041	0.0
25	5.6858208704878036E-5	0.0	0.0	14.558260047840497	0.0
26	5.6858208704878036E-5	0.0	0.0	14.89946615827847	0.0
27	5.6858208704878036E-5	0.0	0.0	15.223557947896275	0.0
28	5.6858208704878036E-5	0.0	0.0	15.575282826944651	0.0
29	5.6858208704878036E-5	0.0	0.0	15.931215213437186	0.0
30	5.6858208704878036E-5	0.0	0.0	16.33291845793715	0.0
31	5.6858208704878036E-5	0.0	0.0	16.721714889061108	0.0
32	1.1371641740975607E-4	0.0	0.0	17.07867072331033	0.0
33	1.1371641740975607E-4	0.0	0.0	17.446145326169958	0.0
34	1.1371641740975607E-4	0.0	0.0	17.798666220140202	0.0
35	1.1371641740975607E-4	0.0	0.0	18.196844255700462	0.0
36	1.1371641740975607E-4	0.0	0.0	18.543167604921873	0.0
37	1.1371641740975607E-4	0.0	0.0	18.908367879433307	0.0
38	1.1371641740975607E-4	0.0	0.0	19.26856463157871	0.0
39	1.1371641740975607E-4	0.0	0.0	19.63234345087252	0.0
40	1.1371641740975607E-4	0.0	0.0	20.01187199397758	0.0
41	1.1371641740975607E-4	0.0	0.0	20.380085753550368	0.0
42	1.1371641740975607E-4	0.0	0.0	20.740964804200228	0.0
43	1.7057462611463412E-4	0.0	0.0	21.089050757891492	0.0
44	1.7057462611463412E-4	0.0	0.0	21.428494263859616	0.0
45	1.7057462611463412E-4	0.0	0.0	21.791647642857672	0.0
46	1.7057462611463412E-4	0.0	0.0	22.130863715990973	0.0
47	1.7057462611463412E-4	0.0	0.0	22.501806669581597	0.0
48	1.7057462611463412E-4	0.0	0.0	22.863652309779443	0.0
49	1.7057462611463412E-4	0.0	0.0	23.260579464748194	0.0
50	1.7057462611463412E-4	0.0	0.0	23.653355970481492	0.0
51	1.7057462611463412E-4	0.0	0.0	24.014803603218404	0.0
52	1.7057462611463412E-4	0.0	0.0	24.404396049264225	0.0
53	1.7057462611463412E-4	0.0	0.0	24.772552950628313	0.0
54	1.7057462611463412E-4	0.0	0.0	25.16453344143974	0.0
55	1.7057462611463412E-4	0.0	0.0	25.547473477067093	0.0
56	1.7057462611463412E-4	0.0	0.0	25.914379497839672	0.0
57	1.7057462611463412E-4	0.0	0.0	26.274576249985074	0.0
58	1.7057462611463412E-4	0.0	0.0	26.605377308230054	0.0
59	1.7057462611463412E-4	0.0	0.0	26.967393523054014	0.0
60	1.7057462611463412E-4	0.0	0.0	27.34243026767139	0.0
61	1.7057462611463412E-4	0.0	0.0	27.744645236049696	0.0
62	1.7057462611463412E-4	0.0	0.0	28.161813913317385	0.0
63	1.7057462611463412E-4	0.0	0.0	28.636068232124774	0.0
64	2.2743283481951214E-4	0.0	0.0	29.046243349721763	0.0
65	3.4114925222926824E-4	0.0	0.0	29.40928301230241	0.0
66	3.4114925222926824E-4	0.0	0.0	29.750261689905564	0.0
67	3.4114925222926824E-4	0.0	0.0	30.0945381436136	0.0
68	3.4114925222926824E-4	0.0	0.0	30.489645835903797	0.0
69	3.4114925222926824E-4	0.0	0.0	30.892997968456203	0.0
70	3.9800746093414624E-4	0.0	0.0	31.243756257956594	0.0
71	3.9800746093414624E-4	0.0	0.0	31.621749629426624	0.0
72	3.9800746093414624E-4	0.0	0.0	31.986267605433596	0.0
73	3.9800746093414624E-4	0.0	0.0	32.34697608145734	0.0
74	3.9800746093414624E-4	0.0	0.0	32.74447181851315	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTACCG	20	4.091505E-5	80.0	1
CGAAACG	260	0.0	76.92308	80
CGTTTTT	8705	0.0	74.89948	1
GGCGATT	570	0.0	71.57894	8
TGTTGCG	165	0.0	70.303024	1
TAGGGTA	1035	0.0	69.95169	5
CGCCGGG	740	0.0	69.189186	2
GATTGCG	110	0.0	69.090904	1
CGGGTAT	250	0.0	68.8	6
TAGGGCG	390	0.0	68.71795	5
GTTAGCG	35	1.181852E-7	68.57143	1
AGTACGG	275	0.0	68.36364	2
GTAAGCG	230	0.0	67.82609	1
ACGTTAG	65	0.0	67.69231	1
AATGCGG	195	0.0	67.69231	2
AAGTGCG	65	0.0	67.69231	1
CGGGTAC	120	0.0	66.666664	6
GTGCGCG	30	3.7964219E-6	66.666664	1
AACGTTG	110	0.0	65.454544	1
ACGGGAT	495	0.0	65.454544	5
>>END_MODULE