FastQCFastQC Report
Thu 26 May 2016
SRR1547285_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547285_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2073453
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136860.6600583664061833No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAG24680.11902849980202107No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69800.070.716341
GGGCGAT13700.065.693437
CGTTAGG4050.065.185182
TACGGGA6950.064.460434
ACGGGAT6450.063.8759735
GGCGATA4000.063.08
CGAGGGA13500.062.222224
ATAGCGG4200.061.9047662
TATAGCG1700.061.176471
TGTAGCG1700.061.176471
AGGGATC21650.060.969986
TAGGGAC17400.060.6896555
GTACGGG7450.060.6711433
TGCGGGA16100.059.8757744
CGGCATT3550.059.7183180
TAGGGAT29550.059.6954355
AAGTACT45000.058.93333480
AGTAGGG26350.058.747633
AGTGCGG4850.058.55672
CGATAGG6150.058.536592
Produced by FastQC (version 0.11.5)