##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547285_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2073453
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02967417153897	33.0	31.0	34.0	31.0	34.0
2	32.43521217987579	34.0	31.0	34.0	31.0	34.0
3	32.534349705539505	34.0	31.0	34.0	31.0	34.0
4	36.068752462679406	37.0	35.0	37.0	35.0	37.0
5	36.034731918206006	37.0	35.0	37.0	35.0	37.0
6	36.083990329175535	37.0	35.0	37.0	35.0	37.0
7	36.315659433804385	37.0	36.0	37.0	35.0	37.0
8	36.303905128305296	37.0	37.0	37.0	35.0	37.0
9	38.06079038203422	39.0	39.0	39.0	35.0	39.0
10-11	37.656403593426035	39.0	37.5	39.0	35.0	39.0
12-13	36.73844234713785	39.0	35.0	39.0	33.0	39.0
14-15	37.673306315600115	40.0	35.0	41.0	33.0	41.0
16-17	37.96039915059565	40.0	35.0	41.0	33.5	41.0
18-19	37.86469334004677	40.0	36.0	41.0	34.0	41.0
20-21	37.65948492683461	40.0	35.0	41.0	33.0	41.0
22-23	37.5369188498606	39.0	35.0	41.0	33.0	41.0
24-25	37.496223931769855	39.0	35.0	41.0	33.0	41.0
26-27	37.473078724234405	39.0	35.0	41.0	33.5	41.0
28-29	37.41117498202274	39.0	35.0	41.0	33.0	41.0
30-31	37.2393574872447	39.0	35.0	41.0	33.0	41.0
32-33	36.95758765691819	39.0	35.0	41.0	32.5	41.0
34-35	36.64961154171327	39.0	35.0	41.0	31.5	41.0
36-37	36.46079510844953	39.0	35.0	41.0	31.0	41.0
38-39	36.36578789102044	39.0	35.0	41.0	31.0	41.0
40-41	36.21025579070276	39.0	35.0	41.0	30.0	41.0
42-43	36.184077237342734	38.5	35.0	41.0	30.0	41.0
44-45	36.06972451268488	38.0	35.0	41.0	30.0	41.0
46-47	35.96715961249182	38.0	35.0	40.5	30.0	41.0
48-49	35.868988108242625	38.0	35.0	40.0	30.0	41.0
50-51	35.63447374982698	37.5	35.0	40.0	29.5	41.0
52-53	35.43254874839218	37.0	35.0	40.0	29.0	41.0
54-55	35.22016679423165	36.5	34.5	40.0	29.0	41.0
56-57	34.913511422732995	36.0	34.0	40.0	27.5	41.0
58-59	34.68330581884422	36.0	34.0	39.5	27.0	41.0
60-61	34.337626172380084	35.0	34.0	39.0	26.5	41.0
62-63	33.94569469382716	35.0	33.5	39.0	26.0	40.0
64-65	33.602314834240275	35.0	33.0	38.0	25.0	40.0
66-67	33.29719265399312	35.0	33.0	37.5	24.5	40.0
68-69	32.81840461298134	35.0	33.0	37.0	22.5	39.5
70-71	32.57778715022718	35.0	33.0	36.5	22.5	39.0
72-73	32.30741304480979	35.0	33.0	36.0	20.5	39.0
74-75	32.027614322581705	35.0	32.5	35.5	20.0	38.0
76-77	30.099245075726337	33.0	29.5	34.5	18.5	36.0
78-79	31.12628571759283	34.0	31.5	35.0	19.0	37.0
80-81	31.28236690197463	35.0	32.0	35.0	18.5	36.0
82-83	31.119606038815444	34.5	32.0	35.0	18.0	36.0
84-85	30.897013821871052	34.0	32.0	35.0	17.0	36.0
86	30.509917514407128	34.0	31.0	35.0	15.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	9.0
10	13.0
11	18.0
12	47.0
13	78.0
14	315.0
15	852.0
16	2079.0
17	4515.0
18	9308.0
19	14337.0
20	16983.0
21	17391.0
22	16188.0
23	14953.0
24	15669.0
25	17520.0
26	20868.0
27	24962.0
28	30678.0
29	38769.0
30	48609.0
31	59280.0
32	70218.0
33	93437.0
34	250969.0
35	213445.0
36	164149.0
37	292446.0
38	432211.0
39	202442.0
40	691.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.4883631314527	22.741388398965398	29.472864829827344	13.297383639754553
2	32.60247519475966	24.685150808819877	29.33377317932936	13.378600817091105
3	29.621795140762774	24.871458383672067	31.63153445002129	13.875212025543862
4	26.982429792235465	27.228782132992645	30.867205574469253	14.921582500302636
5	24.787106339039276	30.81945913411107	29.939670684601964	14.45376384224769
6	23.116800814872583	39.97872148536764	26.420806258931357	10.483671440828415
7	85.87737460169099	4.200818634422868	7.721129921922514	2.2006768419636233
8	85.96322173688046	3.575388494458278	7.895621458504245	2.5657683101570186
9	81.40001244301173	6.315889484835201	9.019736642209878	3.2643614299431913
10-11	42.26203825213304	26.11493966827317	20.00040512131213	11.622616958281668
12-13	30.909140453147476	25.716594492375762	27.638292259337444	15.735972795139316
14-15	17.126913414482992	30.151153655279384	37.052298749959604	15.669634180278019
16-17	23.490476996584924	24.56723156975345	32.26952817353468	19.672763260126946
18-19	25.395921682333768	26.060320634227057	30.70742379981606	17.836333883623116
20-21	25.51639704396483	27.762336546813454	30.338618719594802	16.382647689626918
22-23	21.409841457703646	24.35615854326093	32.941161434573154	21.292838564462276
24-25	20.853933028624233	26.511162780154653	33.62215106877272	19.012753122448398
26-27	18.425351334223635	31.810631830092124	30.37599598351156	19.38802085217268
28-29	17.597794596742727	28.413038540058537	34.14024817538666	19.84891868781207
30-31	23.29300447128534	27.487288113113728	31.114980662691654	18.10472675290928
32-33	26.883560900584673	27.654207739456837	28.205655011230057	17.256576348728426
34-35	20.598320772161223	28.60735208369806	30.769807659011324	20.024519485129392
36-37	23.583944270740645	29.42982069041353	29.410890914816974	17.575344124028856
38-39	20.756655684985382	30.23871291029987	28.189305472561955	20.81532593215279
40-41	21.313528688617488	26.417430247996943	28.925227627537254	23.34381343584832
42-43	21.050344521915857	25.592188489442492	30.224895379832578	23.132571608809073
44-45	19.705245308188804	30.195162369245892	28.359239394382225	21.740352928183086
46-47	20.604783421664248	29.152891336336058	29.132900528731543	21.109424713268158
48-49	21.82979310358132	26.64873522573215	31.458176288538976	20.063295382147558
50-51	19.501527162660548	30.822955716864577	28.41168813568477	21.263828984790106
52-53	22.186058714617594	26.429825030999016	30.45694790284612	20.92716835153727
54-55	21.99256505934786	26.491364887460676	31.014351422482207	20.501718630709256
56-57	21.798902603531403	25.848741206094374	29.835400175456112	22.51695601491811
58-59	23.403231228294057	28.217229905862347	28.132154430315037	20.24738443552856
60-61	23.596700769199977	28.670073543986767	27.370068190598	20.36315749621525
62-63	22.242655126496718	26.368984491088053	30.601489399566812	20.78687098284842
64-65	20.42865210834294	29.57677362351594	30.43671595160344	19.55785831653768
66-67	19.746625556499232	29.282240783851865	26.85474423582305	24.11638942382586
68-69	18.819355924633932	30.78690474295776	27.360856503619807	23.032882828788498
70-71	20.003322959334017	29.877190367951435	29.037552334198075	21.081934338516476
72-73	22.186975060442656	29.738629233457424	27.440409789852964	20.633985916246957
74-75	21.052635386478496	31.68675634316283	25.76043440579555	21.50017386456312
76-77	24.11834268729506	28.99766717644432	27.11351547394612	19.770474662314506
78-79	19.261010497947144	29.952909470337648	29.73240772759257	21.05367230412264
80-81	17.567386383969158	29.56117163012617	30.425719801702762	22.44572218420191
82-83	18.8140025358665	28.32256144701616	31.173506223676156	21.68992979344118
84-85	17.548818323829863	28.036589206507212	30.11269124499084	24.301901224672083
86	18.697216671899483	27.96369148468762	30.84014925826628	22.498942585146615
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	772.0
1	1246.0
2	4716.5
3	5076.5
4	2541.0
5	3013.5
6	3776.5
7	4168.0
8	4484.5
9	4654.5
10	4455.5
11	4374.5
12	4432.5
13	4519.5
14	4520.0
15	4569.5
16	4651.0
17	4770.0
18	5450.0
19	6205.0
20	6885.0
21	7403.0
22	8060.0
23	9214.0
24	10986.0
25	12724.0
26	15216.0
27	18440.5
28	21022.5
29	22409.0
30	26136.0
31	29733.0
32	32225.0
33	38012.0
34	43126.5
35	48621.0
36	52166.0
37	54275.0
38	61644.5
39	71233.5
40	81364.0
41	93394.0
42	104602.5
43	109583.0
44	109081.0
45	108990.0
46	111768.5
47	115629.5
48	112286.0
49	99362.0
50	91275.0
51	81418.5
52	67662.0
53	62464.0
54	59214.0
55	56541.5
56	53793.0
57	51765.0
58	47819.0
59	37320.0
60	26371.5
61	18834.5
62	13045.0
63	8909.5
64	7422.0
65	6444.0
66	4837.0
67	3569.0
68	2552.5
69	1865.5
70	1187.5
71	819.0
72	729.5
73	536.0
74	336.5
75	232.5
76	177.5
77	94.0
78	42.0
79	27.0
80	30.5
81	22.5
82	13.0
83	11.5
84	6.0
85	5.0
86	6.0
87	5.0
88	2.5
89	1.5
90	2.5
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2073453.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.232881503346768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.94686422537461	14.394288204820613
2	8.860170401814312	3.2309287407148135
3	2.9129297037132384	1.5933330634614746
4	1.4203033785658548	1.0358489280077718
5	0.8204640725364181	0.7479712106154909
6	0.5318122986648771	0.5817882374147499
7	0.4170019101341206	0.5322202489901274
8	0.30490593373546937	0.4447451007572892
9	0.2481844739591344	0.40726062942006136
>10	2.675210129435894	11.431730274636058
>50	1.2236330133231688	16.338187875293784
>100	1.6334575397782645	47.70969653864651
>500	0.0026646941919340715	0.30943820538604794
>1k	0.002131755353547257	0.5776277003979783
>5k	0.0	0.0
>10k+	2.664694191934071E-4	0.6649350414371883
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13686	0.6600583664061833	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAG	2468	0.11902849980202107	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.645745526906084E-5	0.0	0.0	0.07393463946373513	0.0
2	9.645745526906084E-5	0.0	0.0	0.23550087703941203	0.0
3	9.645745526906084E-5	0.0	0.0	0.3435814556683947	0.0
4	9.645745526906084E-5	0.0	0.0	0.5186999657093746	0.0
5	9.645745526906084E-5	0.0	0.0	0.8727470552744625	0.0
6	9.645745526906084E-5	0.0	0.0	1.1543545959324855	0.0
7	9.645745526906084E-5	0.0	0.0	1.3491986555759885	0.0
8	9.645745526906084E-5	0.0	0.0	1.742600386890853	0.0
9	9.645745526906084E-5	0.0	0.0	1.862786376156103	0.0
10	9.645745526906084E-5	0.0	0.0	2.1021455514062772	0.0
11	9.645745526906084E-5	0.0	0.0	2.4913513834169376	0.0
12	9.645745526906084E-5	0.0	0.0	2.79615694206717	0.0
13	9.645745526906084E-5	0.0	0.0	2.926615650318575	0.0
14	9.645745526906084E-5	0.0	0.0	2.98309149037861	0.0
15	9.645745526906084E-5	0.0	0.0	3.0727004663235675	0.0
16	9.645745526906084E-5	0.0	0.0	3.2496516680146597	0.0
17	9.645745526906084E-5	0.0	0.0	3.4674043732845643	0.0
18	9.645745526906084E-5	0.0	0.0	3.722437885015961	0.0
19	9.645745526906084E-5	0.0	0.0	3.896254219410809	0.0
20	9.645745526906084E-5	0.0	0.0	4.079185783328583	0.0
21	9.645745526906084E-5	0.0	0.0	4.298433579155158	0.0
22	9.645745526906084E-5	0.0	0.0	4.526169631045411	0.0
23	9.645745526906084E-5	0.0	0.0	4.740160495559822	0.0
24	9.645745526906084E-5	0.0	0.0	4.915761292877147	0.0
25	9.645745526906084E-5	0.0	0.0	5.076073583534327	0.0
26	9.645745526906084E-5	0.0	0.0	5.239617198943019	0.0
27	9.645745526906084E-5	0.0	0.0	5.453511606002162	0.0
28	9.645745526906084E-5	0.0	0.0	5.63287424407498	0.0
29	1.4468618290359124E-4	0.0	0.0	5.8888241016314335	0.0
30	1.4468618290359124E-4	0.0	0.0	6.135369357299153	0.0
31	1.4468618290359124E-4	0.0	0.0	6.347961588712162	0.0
32	1.4468618290359124E-4	0.0	0.0	6.534896137023603	0.0
33	1.4468618290359124E-4	0.0	0.0	6.723277547164079	0.0
34	1.4468618290359124E-4	0.0	0.0	6.945901353925071	0.0
35	1.4468618290359124E-4	0.0	0.0	7.1780744487577	0.0
36	1.4468618290359124E-4	0.0	0.0	7.3872424405086585	0.0
37	1.4468618290359124E-4	0.0	0.0	7.620958854625593	0.0
38	1.4468618290359124E-4	0.0	0.0	7.912308598265791	0.0
39	1.4468618290359124E-4	0.0	0.0	8.503689256520403	0.0
40	1.4468618290359124E-4	0.0	0.0	8.772178583261834	0.0
41	1.4468618290359124E-4	0.0	0.0	9.036327324516158	0.0
42	1.4468618290359124E-4	0.0	0.0	9.298209315571658	0.0
43	1.4468618290359124E-4	0.0	0.0	9.518373457223289	0.0
44	1.9291491053812168E-4	0.0	0.0	9.745144934560852	0.0
45	2.411436381726521E-4	0.0	0.0	9.963187012196563	0.0
46	2.893723658071825E-4	0.0	0.0	10.207031459116749	0.0
47	3.3760109344171295E-4	0.0	0.0	10.478221594605714	0.0
48	3.8582982107624337E-4	0.0	0.0	10.726985371744622	0.0
49	3.8582982107624337E-4	0.0	0.0	10.978064127809986	0.0
50	3.8582982107624337E-4	0.0	0.0	11.249736550575296	0.0
51	3.8582982107624337E-4	0.0	0.0	11.513210089642737	0.0
52	3.8582982107624337E-4	0.0	0.0	11.87637240873075	0.0
53	3.8582982107624337E-4	0.0	0.0	12.145344022748526	0.0
54	3.8582982107624337E-4	0.0	0.0	12.423672009927401	0.0
55	3.8582982107624337E-4	0.0	0.0	12.683431936966983	0.0
56	4.340585487107738E-4	0.0	0.0	12.950763774245184	0.0
57	4.340585487107738E-4	0.0	0.0	13.22378660138426	0.0
58	4.340585487107738E-4	0.0	0.0	13.465991271564873	0.0
59	4.822872763453042E-4	0.0	0.0	13.71986729383304	0.0
60	5.305160039798346E-4	0.0	0.0	14.028965209242747	0.0
61	5.305160039798346E-4	0.0	0.0	14.351711854573024	0.0
62	5.305160039798346E-4	0.0	0.0	14.736866473462383	0.0
63	5.305160039798346E-4	0.0	0.0	15.074033508355386	0.0
64	5.78744731614365E-4	0.0	0.0	15.403049888278153	0.0
65	5.78744731614365E-4	0.0	0.0	15.696907525755346	0.0
66	5.78744731614365E-4	0.0	0.0	15.979142039872618	0.0
67	5.78744731614365E-4	0.0	0.0	16.281295018502952	0.0
68	5.78744731614365E-4	0.0	0.0	16.690660458664844	0.0
69	6.269734592488955E-4	0.0	0.0	17.380090120200457	0.0
70	6.269734592488955E-4	0.0	0.0	17.721356596942396	0.0
71	6.269734592488955E-4	0.0	0.0	18.020712309369927	0.0
72	6.269734592488955E-4	0.0	0.0	18.334391953904912	0.0
73	6.269734592488955E-4	0.0	0.0	18.64787868352936	0.0
74	6.269734592488955E-4	0.0	0.0	18.994643235221634	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	6980	0.0	70.71634	1
GGGCGAT	1370	0.0	65.69343	7
CGTTAGG	405	0.0	65.18518	2
TACGGGA	695	0.0	64.46043	4
ACGGGAT	645	0.0	63.875973	5
GGCGATA	400	0.0	63.0	8
CGAGGGA	1350	0.0	62.22222	4
ATAGCGG	420	0.0	61.904766	2
TATAGCG	170	0.0	61.17647	1
TGTAGCG	170	0.0	61.17647	1
AGGGATC	2165	0.0	60.96998	6
TAGGGAC	1740	0.0	60.689655	5
GTACGGG	745	0.0	60.671143	3
TGCGGGA	1610	0.0	59.875774	4
CGGCATT	355	0.0	59.71831	80
TAGGGAT	2955	0.0	59.695435	5
AAGTACT	4500	0.0	58.933334	80
AGTAGGG	2635	0.0	58.74763	3
AGTGCGG	485	0.0	58.5567	2
CGATAGG	615	0.0	58.53659	2
>>END_MODULE