FastQCFastQC Report
Thu 26 May 2016
SRR1547284_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547284_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2682418
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155980.5814902822751712No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCC27370.10203480591019No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95150.072.22281
TACGGGA9850.071.472084
ACGGGAT11850.070.2109765
GGCGATA3800.064.2105268
GGGCGAT14650.064.163827
CGGGATC7250.063.4482776
TATAGCG950.063.1578941
GCGGGAT18150.063.0303045
CGAGGGA18150.061.9283754
TAGGGAT57800.061.5916945
TAAGGGA44900.061.3808484
TGCGGGA23000.061.043484
TAGGGAC33700.061.00895
ATAGGGA48750.060.4717944
GTAGGGA46050.060.456024
AGGGATT63350.060.42626
AGGGATC42250.060.3076936
CGGGATG10800.059.6296276
ACGAGGG13750.059.0545463
GTACGGG9300.058.924733
Produced by FastQC (version 0.11.5)