##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547284_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2682418
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.116050518599266	33.0	31.0	34.0	31.0	34.0
2	32.55030200364	34.0	31.0	34.0	31.0	34.0
3	32.65982520248522	34.0	31.0	34.0	31.0	34.0
4	36.18274034844681	37.0	35.0	37.0	35.0	37.0
5	36.16265921269541	37.0	35.0	37.0	35.0	37.0
6	36.21962125216875	37.0	37.0	37.0	35.0	37.0
7	36.38189424616149	37.0	37.0	37.0	35.0	37.0
8	36.36738010257909	37.0	37.0	37.0	35.0	37.0
9	38.137141191268476	39.0	39.0	39.0	37.0	39.0
10-11	37.79722977552343	39.0	37.5	39.0	35.0	39.0
12-13	37.187859237449196	39.0	37.0	39.0	34.5	39.0
14-15	38.39528309905466	40.0	37.0	41.0	34.0	41.0
16-17	38.52023920209304	40.0	37.0	41.0	34.5	41.0
18-19	38.36475560483116	40.0	37.0	41.0	34.0	41.0
20-21	38.1976573002418	40.0	35.5	41.0	34.0	41.0
22-23	38.02807299235242	40.0	35.0	41.0	34.0	41.0
24-25	38.018492270779575	40.0	35.0	41.0	34.0	41.0
26-27	38.00535487012091	40.0	35.0	41.0	34.0	41.0
28-29	37.90767509016119	40.0	35.0	41.0	34.0	41.0
30-31	37.77838241467214	40.0	35.5	41.0	34.0	41.0
32-33	37.457790322015434	40.0	35.0	41.0	33.0	41.0
34-35	37.143590782644615	40.0	35.0	41.0	32.5	41.0
36-37	36.953858235368244	40.0	35.0	41.0	32.0	41.0
38-39	36.86419715346378	40.0	35.0	41.0	32.0	41.0
40-41	36.74009885856716	40.0	35.0	41.0	31.0	41.0
42-43	36.68463658534949	40.0	35.0	41.0	31.0	41.0
44-45	36.562815713285545	39.0	35.0	41.0	31.0	41.0
46-47	36.420710344174545	39.0	35.0	41.0	31.0	41.0
48-49	36.32351911596179	39.0	35.0	41.0	31.0	41.0
50-51	36.10415546719415	38.5	35.0	40.5	30.5	41.0
52-53	35.90122661717898	38.0	35.0	40.0	30.0	41.0
54-55	35.73058673182181	38.0	35.0	40.0	29.5	41.0
56-57	35.46302738797607	37.0	35.0	40.0	29.0	41.0
58-59	35.231988079411934	37.0	35.0	40.0	29.0	41.0
60-61	34.892721604164606	36.0	34.0	40.0	28.0	41.0
62-63	34.526879293234686	36.0	34.0	39.0	27.0	41.0
64-65	34.21836622778403	35.0	34.0	39.0	26.5	40.0
66-67	33.93026534268708	35.0	34.0	38.5	26.0	40.0
68-69	33.46374744726586	35.0	33.5	37.5	25.5	40.0
70-71	33.16628392741176	35.0	33.0	37.0	25.0	39.0
72-73	32.901652166068075	35.0	33.0	36.5	25.0	39.0
74-75	32.61011035565672	35.0	33.0	36.0	24.5	38.5
76-77	30.68344456382264	33.5	30.0	34.5	20.5	36.0
78-79	31.736756165519317	34.5	32.0	35.0	22.5	37.0
80-81	31.869467771242213	35.0	33.0	35.0	23.0	37.0
82-83	31.687327068339087	35.0	33.0	35.0	21.0	36.0
84-85	31.471855803234245	35.0	32.0	35.0	20.0	36.0
86	31.050163695591067	34.0	31.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	13.0
10	15.0
11	28.0
12	34.0
13	119.0
14	342.0
15	985.0
16	2260.0
17	5425.0
18	11785.0
19	19958.0
20	24109.0
21	24223.0
22	20763.0
23	17339.0
24	15942.0
25	16852.0
26	19373.0
27	23014.0
28	28334.0
29	36386.0
30	45903.0
31	58386.0
32	73991.0
33	99571.0
34	259398.0
35	246578.0
36	231920.0
37	434977.0
38	658936.0
39	304821.0
40	636.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.646464495839204	23.797036852571075	26.973760241692386	14.582738409897338
2	32.01130472581082	25.31152862827494	29.205739001154928	13.471427644759318
3	28.667493284044472	25.75467358182058	31.965152336436752	13.612680797698196
4	26.19375503743264	27.751267699515886	31.1725092808056	14.88246798224587
5	24.68291668188925	31.69315893346973	29.44839320344555	14.175531181195474
6	22.820007918229003	41.05948439057597	25.907856269977316	10.212651421217721
7	87.33541901374059	3.6087589629953274	7.522280271009215	1.5335417522548684
8	87.59346977242176	3.7998924850638494	6.642961686060859	1.9636760564535432
9	82.78657539578097	5.8778311210258805	8.046210545858251	3.289382937334897
10-11	37.6588585373346	29.669835200926926	20.71774421436182	11.953562047376657
12-13	29.04191293079602	23.728665703853764	29.131048926751905	18.09837243859831
14-15	18.539783881557607	29.98537140743911	34.03634332904119	17.438501381962094
16-17	23.318643850436434	26.594028223789135	31.61393936366368	18.473388562110753
18-19	24.184318029479375	27.517616568334986	30.08110592756237	18.216959474623266
20-21	26.544222414254605	27.847114059031814	29.329191050760915	16.279472475952666
22-23	22.525944875108948	24.052459385524553	32.426228872606735	20.995366866759767
24-25	20.55073072131189	27.607908238015106	32.68032797274697	19.161033067926027
26-27	19.761125969181535	31.957808216318263	29.380525331995237	18.90054048250496
28-29	18.584687397713555	27.966316211716446	34.274393476333664	19.174602914236335
30-31	22.191806049616428	27.55402029064821	30.502740437918323	19.751433221817035
32-33	24.684706112171927	28.17668610932375	28.66663584870069	18.471971929803633
34-35	20.49783068858023	28.75668519969669	30.083827352783942	20.661656758939138
36-37	22.200399042953038	29.935416478714355	28.656421184170398	19.20776329416221
38-39	20.731761418242794	30.381431976671795	28.28768670654611	20.599119898539303
40-41	21.127877907171815	27.78398072187109	28.93050225580055	22.15763911515655
42-43	21.392098472348454	27.849947323646052	28.217805726027784	22.540148477977706
44-45	20.32578442286027	29.864286624977908	28.315944793093394	21.49398415906842
46-47	21.13889408734955	29.649927789032134	28.292383961038137	20.918794162580177
48-49	21.218449175333596	27.1874107614846	30.585221990010503	21.008918073171294
50-51	19.860588469060378	29.845907684782908	28.849064538039933	21.44443930811678
52-53	21.565449530982868	27.315448226189954	29.680534502825434	21.438567740001744
54-55	21.151308259935625	27.386764478914174	30.591000358631653	20.870926902518548
56-57	21.493182643420976	27.092365917616124	29.34039362992643	22.074057809036475
58-59	22.28662721469957	28.155753502996177	28.490656564338597	21.066962717965655
60-61	23.148424294796712	28.82733041606491	27.6175264257845	20.406718863353884
62-63	21.895823096922253	27.27723643369527	29.70800971362405	21.118930755758424
64-65	20.218754124077602	30.025353990317694	29.365482933681474	20.390408951923227
66-67	20.417511364746286	29.469586768356017	26.972269049790153	23.14063281710755
68-69	19.53604919143847	31.516210374371184	26.480828118510985	22.46691231567936
70-71	20.073810271180704	30.155404564091054	28.472519942827702	21.29826522190054
72-73	21.348555668803296	30.741964898833814	27.02009903005423	20.889380402308664
74-75	20.114184291933622	31.40988839174208	26.778339542904945	21.697587773419354
76-77	22.610663215054476	29.007317278664253	27.02371517041714	21.358304335864133
78-79	19.851268519671432	30.3503965452066	28.251562582714552	21.546772352407416
80-81	18.23038020174335	29.847976713547254	29.046200107514935	22.875442977194457
82-83	19.585351723705998	29.37323713157308	29.111327913844896	21.930083230876026
84-85	18.640812132933792	28.83713500282208	28.18609925820659	24.33595360603754
86	18.823986418224152	29.46397615882387	29.46326784267031	22.24876958028167
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	572.0
1	1085.5
2	5139.5
3	5914.0
4	3338.5
5	4058.0
6	4747.5
7	4908.0
8	5179.5
9	5539.0
10	5595.5
11	5635.5
12	5706.0
13	5847.5
14	5990.0
15	6159.5
16	6288.5
17	6545.0
18	6950.0
19	7442.0
20	8359.0
21	8892.0
22	9568.5
23	10707.0
24	12304.0
25	15449.5
26	20446.0
27	25853.5
28	30985.0
29	33695.0
30	36502.0
31	41945.5
32	47318.5
33	52947.0
34	60862.5
35	69282.5
36	72679.0
37	78559.5
38	87407.5
39	96564.5
40	107189.0
41	114505.0
42	122315.5
43	127245.0
44	129280.5
45	131531.5
46	133366.0
47	136031.5
48	136168.0
49	123448.5
50	111639.0
51	107192.5
52	96048.0
53	85529.0
54	78387.5
55	72548.0
56	66353.5
57	62678.0
58	56541.0
59	45102.0
60	35221.0
61	27781.0
62	23291.0
63	18514.5
64	15367.0
65	12369.0
66	8224.0
67	6084.5
68	4215.5
69	2877.5
70	2054.5
71	1693.0
72	1425.5
73	897.5
74	461.0
75	328.0
76	265.0
77	109.0
78	66.0
79	73.0
80	57.5
81	35.0
82	26.0
83	17.5
84	9.5
85	9.5
86	11.0
87	8.0
88	5.5
89	4.5
90	2.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2682418.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.33090527387576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.96517552771897	13.858688822797454
2	7.439683300955234	2.57872893112981
3	2.296090869247566	1.1938000006542187
4	1.100954060073348	0.7632212210408047
5	0.7141704058634578	0.6188609826712496
6	0.4917012158194803	0.5112976316650172
7	0.3663025848004021	0.44438487791261283
8	0.29491104599071616	0.4088860321827775
9	0.258472352451417	0.4031603870626203
>10	3.6580649458797923	16.125944571433653
>50	1.8580996223784279	23.518716740958137
>100	1.5522503615307572	38.349505929992205
>500	0.0023874094839992127	0.24855672183098243
>1k	0.0015192605807267717	0.38953592785739316
>5k	0.0	0.0
>10k+	2.1703722581811023E-4	0.5867112208110872
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15598	0.5814902822751712	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCC	2737	0.10203480591019	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.727979755578735E-5	0.0	0.0	0.02083940683368513	0.0
2	3.727979755578735E-5	0.0	0.0	0.07601350721625041	0.0
3	3.727979755578735E-5	0.0	0.0	0.1110937967162463	0.0
4	3.727979755578735E-5	0.0	0.0	0.18024782118223184	0.0
5	3.727979755578735E-5	0.0	0.0	0.37950833911791526	0.0
6	3.727979755578735E-5	0.0	0.0	0.5061105316173691	0.0
7	3.727979755578735E-5	0.0	0.0	0.6126189132342535	0.0
8	3.727979755578735E-5	0.0	0.0	0.838348087434546	0.0
9	3.727979755578735E-5	0.0	0.0	0.9206246006401687	0.0
10	3.727979755578735E-5	0.0	0.0	1.172673311914847	0.0
11	3.727979755578735E-5	0.0	0.0	1.414581918254351	0.0
12	3.727979755578735E-5	0.0	0.0	1.6478043317633568	0.0
13	3.727979755578735E-5	0.0	0.0	1.7178530713706812	0.0
14	3.727979755578735E-5	0.0	0.0	1.7523368841097846	0.0
15	3.727979755578735E-5	0.0	0.0	1.8100087309285875	0.0
16	3.727979755578735E-5	0.0	0.0	1.9013442349402665	0.0
17	3.727979755578735E-5	0.0	0.0	2.0119533942882875	0.0
18	3.727979755578735E-5	0.0	0.0	2.139748540309527	0.0
19	3.727979755578735E-5	0.0	0.0	2.247897233018866	0.0
20	3.727979755578735E-5	0.0	0.0	2.348850924799938	0.0
21	3.727979755578735E-5	0.0	0.0	2.47537855770428	0.0
22	3.727979755578735E-5	0.0	0.0	2.624609587320097	0.0
23	3.727979755578735E-5	0.0	0.0	2.76962799981211	0.0
24	3.727979755578735E-5	0.0	0.0	2.880237159160131	0.0
25	3.727979755578735E-5	0.0	0.0	2.986074504421011	0.0
26	3.727979755578735E-5	0.0	0.0	3.0887803466872055	0.0
27	3.727979755578735E-5	0.0	0.0	3.1962207232429845	0.0
28	3.727979755578735E-5	0.0	0.0	3.309215789634576	0.0
29	3.727979755578735E-5	0.0	0.0	3.437868370999598	0.0
30	3.727979755578735E-5	0.0	0.0	3.5854218097254043	0.0
31	3.727979755578735E-5	0.0	0.0	3.723618019264708	0.0
32	3.727979755578735E-5	0.0	0.0	3.849549175408158	0.0
33	3.727979755578735E-5	0.0	0.0	3.9751075335760495	0.0
34	3.727979755578735E-5	0.0	0.0	4.11457125623225	0.0
35	3.727979755578735E-5	0.0	0.0	4.270773607990999	0.0
36	3.727979755578735E-5	0.0	0.0	4.414226268985669	0.0
37	3.727979755578735E-5	0.0	0.0	4.57102509750531	0.0
38	3.727979755578735E-5	0.0	0.0	4.7390824248868	0.0
39	3.727979755578735E-5	0.0	0.0	4.957877556741716	0.0
40	3.727979755578735E-5	0.0	0.0	5.132496128493024	0.0
41	3.727979755578735E-5	0.0	0.0	5.3171429657868385	0.0
42	3.727979755578735E-5	0.0	0.0	5.497465346564182	0.0
43	3.727979755578735E-5	0.0	0.0	5.662689409331431	0.0
44	3.727979755578735E-5	0.0	0.0	5.832461607400488	0.0
45	3.727979755578735E-5	0.0	0.0	5.997573830775069	0.0
46	7.45595951115747E-5	0.0	0.0	6.183637300376004	0.0
47	7.45595951115747E-5	0.0	0.0	6.376560252727203	0.0
48	7.45595951115747E-5	0.0	0.0	6.563294758684142	0.0
49	7.45595951115747E-5	0.0	0.0	6.7521169333042055	0.0
50	7.45595951115747E-5	0.0	0.0	6.953800638081015	0.0
51	7.45595951115747E-5	0.0	0.0	7.15526066407249	0.0
52	7.45595951115747E-5	0.0	0.0	7.372191806049616	0.0
53	7.45595951115747E-5	0.0	0.0	7.575403982526213	0.0
54	1.491191902231494E-4	0.0	0.0	7.7788025579905895	0.0
55	1.491191902231494E-4	0.0	0.0	7.98622735159099	0.0
56	1.491191902231494E-4	0.0	0.0	8.194695979522953	0.0
57	1.491191902231494E-4	0.0	0.0	8.406967146805606	0.0
58	1.491191902231494E-4	0.0	0.0	8.60462463344639	0.0
59	1.491191902231494E-4	0.0	0.0	8.812534064415017	0.0
60	1.491191902231494E-4	0.0	0.0	9.02860777104836	0.0
61	1.491191902231494E-4	0.0	0.0	9.264775288564273	0.0
62	1.491191902231494E-4	0.0	0.0	9.51346881805893	0.0
63	2.236787853347241E-4	0.0	0.0	9.774874758520111	0.0
64	2.236787853347241E-4	0.0	0.0	10.016522406276724	0.0
65	2.236787853347241E-4	0.0	0.0	10.249334742012618	0.0
66	2.236787853347241E-4	0.0	0.0	10.466079485001964	0.0
67	2.236787853347241E-4	0.0	0.0	10.689199073373352	0.0
68	2.6095858289051146E-4	0.0	0.0	10.942440738169816	0.0
69	2.6095858289051146E-4	0.0	0.0	11.248023238734604	0.0
70	2.6095858289051146E-4	0.0	0.0	11.512262443810025	0.0
71	2.6095858289051146E-4	0.0	0.0	11.757638071322217	0.0
72	2.6095858289051146E-4	0.0	0.0	11.997608128188821	0.0
73	2.6095858289051146E-4	0.0	0.0	12.24920948189283	0.0
74	2.6095858289051146E-4	0.0	0.0	12.523998869676538	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	9515	0.0	72.2228	1
TACGGGA	985	0.0	71.47208	4
ACGGGAT	1185	0.0	70.210976	5
GGCGATA	380	0.0	64.210526	8
GGGCGAT	1465	0.0	64.16382	7
CGGGATC	725	0.0	63.448277	6
TATAGCG	95	0.0	63.157894	1
GCGGGAT	1815	0.0	63.030304	5
CGAGGGA	1815	0.0	61.928375	4
TAGGGAT	5780	0.0	61.591694	5
TAAGGGA	4490	0.0	61.380848	4
TGCGGGA	2300	0.0	61.04348	4
TAGGGAC	3370	0.0	61.0089	5
ATAGGGA	4875	0.0	60.471794	4
GTAGGGA	4605	0.0	60.45602	4
AGGGATT	6335	0.0	60.4262	6
AGGGATC	4225	0.0	60.307693	6
CGGGATG	1080	0.0	59.629627	6
ACGAGGG	1375	0.0	59.054546	3
GTACGGG	930	0.0	58.92473	3
>>END_MODULE