FastQCFastQC Report
Thu 26 May 2016
SRR1547283_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547283_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2060842
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127180.6171263978509754No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76300.074.914811
TAGCGCG1150.073.043481
ACGGGTA4650.072.2580645
CGTAAGG3400.070.5882342
TACGGGT6950.067.9136664
TAGGGTA21250.067.0117655
AGACGCG850.065.8823551
GGGCGAT12800.065.6257
CTATGCG559.094947E-1265.4545441
GCGGGTC9650.064.6632165
GACGGGT6750.064.592594
TCGATAG1500.064.01
TATAGCG1000.064.01
CGGGTAG4600.063.4782646
TGCGGGT13400.063.2835854
CTACGGG3800.063.1578943
TTAGCGG4450.062.9213452
ACGGGTG8500.062.5882345
TATTGCG2950.062.372881
GTGCGGG12850.062.256813
Produced by FastQC (version 0.11.5)