##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547283_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2060842
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.951255845911525	33.0	31.0	33.0	30.0	34.0
2	32.38097534891079	33.0	31.0	34.0	31.0	34.0
3	32.52513729824994	34.0	31.0	34.0	31.0	34.0
4	36.073063340129906	37.0	35.0	37.0	35.0	37.0
5	36.03382889129783	37.0	35.0	37.0	35.0	37.0
6	36.08611916876694	37.0	35.0	37.0	35.0	37.0
7	36.335892804979714	37.0	36.0	37.0	35.0	37.0
8	36.34460526328559	37.0	37.0	37.0	35.0	37.0
9	38.148055018288645	39.0	39.0	39.0	37.0	39.0
10-11	37.53834719012909	39.0	37.0	39.0	35.0	39.0
12-13	37.011629227277005	39.0	35.0	39.0	33.5	39.0
14-15	38.14928461279419	40.0	36.0	41.0	33.0	41.0
16-17	38.330734961729235	40.0	36.0	41.0	34.0	41.0
18-19	38.20608954980537	40.0	36.0	41.0	34.0	41.0
20-21	38.02620700665068	40.0	35.0	41.0	34.0	41.0
22-23	37.82700517555446	40.0	35.0	41.0	33.5	41.0
24-25	37.797017675299706	40.0	35.0	41.0	34.0	41.0
26-27	37.78586470966721	40.0	35.0	41.0	34.0	41.0
28-29	37.717268475700706	40.0	35.0	41.0	33.5	41.0
30-31	37.588963151954395	40.0	35.0	41.0	33.0	41.0
32-33	37.274908750889196	40.0	35.0	41.0	33.0	41.0
34-35	36.98337063200381	40.0	35.0	41.0	32.0	41.0
36-37	36.77693025472113	40.0	35.0	41.0	31.0	41.0
38-39	36.67808279334369	39.5	35.0	41.0	31.0	41.0
40-41	36.547163974724896	39.0	35.0	41.0	31.0	41.0
42-43	36.51722475570665	39.0	35.0	41.0	31.0	41.0
44-45	36.40508272832172	39.0	35.0	41.0	30.5	41.0
46-47	36.27703312529539	39.0	35.0	41.0	30.0	41.0
48-49	36.18220101298401	39.0	35.0	41.0	30.0	41.0
50-51	35.956087123612576	38.0	35.0	40.0	29.5	41.0
52-53	35.774840332252545	38.0	35.0	40.0	29.0	41.0
54-55	35.59760597852723	38.0	35.0	40.0	29.0	41.0
56-57	35.3130140495972	37.0	35.0	40.0	28.5	41.0
58-59	35.07465104069114	37.0	34.0	40.0	28.0	41.0
60-61	34.7185286402354	36.0	34.0	39.5	27.0	41.0
62-63	34.36069310505124	36.0	34.0	39.0	26.0	41.0
64-65	33.989191796362846	35.0	34.0	39.0	26.0	40.0
66-67	33.6846211402912	35.0	34.0	38.0	25.5	40.0
68-69	33.18912197053437	35.0	33.0	37.5	24.0	40.0
70-71	32.900032122792524	35.0	33.0	37.0	24.0	39.0
72-73	32.624078653288315	35.0	33.0	36.5	23.5	39.0
74-75	32.314316429886425	35.0	33.0	36.0	23.0	38.5
76-77	30.339980211971614	33.0	30.0	34.5	19.5	36.0
78-79	31.38620549270638	34.0	31.5	35.0	20.0	37.0
80-81	31.506958078300034	35.0	32.0	35.0	20.0	36.5
82-83	31.311126714226518	34.5	32.0	35.0	19.0	36.0
84-85	31.08889303498279	34.0	32.0	35.0	18.5	36.0
86	30.659187361282427	34.0	31.0	35.0	18.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	8.0
11	14.0
12	17.0
13	73.0
14	284.0
15	824.0
16	2014.0
17	4465.0
18	9527.0
19	16003.0
20	18638.0
21	17609.0
22	15439.0
23	13591.0
24	13648.0
25	15133.0
26	17788.0
27	21961.0
28	26670.0
29	33285.0
30	41497.0
31	51457.0
32	64227.0
33	83890.0
34	200881.0
35	189115.0
36	172936.0
37	324968.0
38	485442.0
39	218782.0
40	652.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.738795113841817	23.94860935481711	31.498387552272323	13.81420797906875
2	29.684759918518743	26.128640623589778	31.489556210519776	12.697043247371706
3	27.982640105354996	25.334887390687882	33.91739881077734	12.76507369317978
4	25.677708431796326	27.10125278890861	32.99869664923366	14.222342130061403
5	24.799378118264283	31.728148009405864	30.597493645801084	12.874980226528768
6	22.279971002143782	40.67264739363814	26.46408603861917	10.583295565598917
7	89.44242207796619	2.9873226574380762	6.254385343466408	1.3158699211293248
8	90.38116459194833	2.343314043483198	6.060969254314498	1.214552110253964
9	86.20578384951394	4.30362929326945	7.730723655670838	1.759863201545776
10-11	36.88213361334833	24.370451495068522	25.760004891204662	12.987410000378485
12-13	29.26141353873805	25.050683167365573	27.498056619575884	18.189846674320496
14-15	18.431616785760383	30.344126332829006	33.843011739861666	17.38124514154894
16-17	22.853134786655165	26.360075153747836	31.345246263420485	19.441543796176514
18-19	23.958605269108453	28.052126266836563	29.682552083080605	18.30671638097438
20-21	25.92234145072742	27.82289957211664	29.243192830891452	17.011566146264485
22-23	22.279655597081195	23.801290928659256	31.78159703655108	22.13745643770847
24-25	20.022714016892124	27.67725521898331	33.051951580955745	19.24807918316882
26-27	19.968585655765946	32.23806094790382	29.384931013634237	18.408422382696006
28-29	17.500783660270898	28.446091451940518	34.10176520082568	19.951359686962903
30-31	21.622812423271654	28.348461454104683	30.376249125357504	19.652476997266167
32-33	24.248729402836318	27.650809717581453	28.74897735973937	19.35148351984286
34-35	20.03906655629107	28.732915963475126	29.742236425693964	21.485781054539842
36-37	22.4528372383715	28.843259211526163	29.359286155852804	19.344617394249536
38-39	20.942022726633095	30.063076160132606	27.980529317628427	21.014371795605875
40-41	21.26177067431661	27.700716503254498	28.081192056450714	22.956320765978177
42-43	21.473868447945062	27.57237090470788	28.148834311412518	22.804926335934535
44-45	20.298183946173456	30.58349451340763	27.75256424315886	21.365757297260053
46-47	21.297799637235652	29.640627471683906	27.45863098675202	21.602941904328425
48-49	21.3468329935046	27.05566462640028	30.1154334005227	21.48206897957243
50-51	19.694498656374435	30.19855476547935	28.353920387880294	21.75302619026592
52-53	21.878387571681866	27.138203705087534	29.32364538378003	21.659763339450574
54-55	21.247164993725864	27.81445642121036	29.841419186914862	21.09695939814891
56-57	21.629508715369738	26.65320776653426	29.226403576790457	22.490879941305543
58-59	22.82038118400149	27.237653347515238	28.370806689692852	21.571158778790416
60-61	22.88913948764631	28.938123349582355	27.272614785607047	20.900122377164283
62-63	22.04048151192571	27.26150282263269	29.014427112801467	21.683588552640135
64-65	19.901913877919803	30.095975334353632	29.39725607300317	20.604854714723402
66-67	19.626177067431662	28.995284451694985	26.89985452548036	24.47868395539299
68-69	19.21818848800636	30.287765874336802	26.191891469603203	24.30215416805364
70-71	19.47990190417315	29.289799994371236	28.143957663906306	23.086340437549314
72-73	21.585449054318577	29.47921286542103	27.233213414711077	21.702124665549324
74-75	21.248038423129962	30.326439387396025	25.867946208394432	22.557575981079577
76-77	23.201536071178673	28.440486946597552	26.83818070477989	21.519796277443877
78-79	19.992750535945987	29.574052741549327	28.70132693336025	21.731869789144437
80-81	17.80655673748885	29.066687305479995	29.416204638686516	23.71055131834464
82-83	19.01244733948551	27.8238700492323	29.901152053384006	23.262530557898177
84-85	18.417277986376444	27.96451644521996	28.65246341058655	24.965742157817047
86	19.308758264825737	27.495945831849312	29.712612611738308	23.482683291586643
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	577.0
1	788.5
2	3813.5
3	4330.5
4	2213.0
5	2823.5
6	3551.0
7	3847.0
8	3988.0
9	4066.0
10	3921.5
11	3812.5
12	3844.5
13	3908.0
14	3912.0
15	3996.5
16	4145.0
17	4415.0
18	4942.5
19	5308.0
20	5472.5
21	5593.0
22	6548.0
23	7742.5
24	8632.0
25	10386.0
26	12783.0
27	15055.0
28	18300.0
29	20566.0
30	22492.5
31	26902.5
32	32648.0
33	36975.0
34	43837.5
35	49378.5
36	49122.0
37	53236.5
38	60007.0
39	66637.5
40	75921.0
41	86721.0
42	98181.0
43	104150.0
44	106455.5
45	112568.5
46	117274.0
47	123034.0
48	121989.0
49	108390.0
50	100698.0
51	92497.5
52	80277.5
53	72117.5
54	65989.5
55	59271.5
56	50625.0
57	46709.0
58	41082.0
59	30099.5
60	21337.5
61	16196.0
62	12620.0
63	9060.0
64	7341.0
65	5759.5
66	3439.5
67	2583.5
68	2109.0
69	1500.0
70	994.0
71	740.0
72	573.0
73	387.0
74	257.5
75	132.5
76	82.0
77	36.0
78	24.0
79	22.0
80	20.0
81	14.5
82	9.0
83	5.5
84	3.0
85	4.0
86	6.0
87	4.0
88	2.5
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2060842.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.940168860008654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.41225646981124	15.834938035623509
2	8.05910964691761	3.2140001444172372
3	2.665362323905931	1.5944332443536806
4	1.275756251178018	1.0175518029080517
5	0.7935290264313755	0.7911551391179947
6	0.5509858376600801	0.6592050385449187
7	0.40834097394510815	0.5699671581048096
8	0.34897169992108984	0.556684369903264
9	0.2701069039950855	0.4847379548324536
>10	3.5080752168940923	16.772725014112886
>50	1.4460246751002837	20.844969837747833
>100	1.2582997419154431	36.615991478280094
>500	0.0024471017882398143	0.3079491759649519
>1k	4.894203576479629E-4	0.11510887436243411
>5k	0.0	0.0
>10k+	2.447101788239814E-4	0.6205827317259165
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12718	0.6171263978509754	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.8523855783218706E-5	0.0	0.0	0.033918175192469875	0.0
2	4.8523855783218706E-5	0.0	0.0	0.10733476899247978	0.0
3	4.8523855783218706E-5	0.0	0.0	0.16173001132546794	0.0
4	4.8523855783218706E-5	0.0	0.0	0.273334879626871	0.0
5	4.8523855783218706E-5	0.0	0.0	0.5183318274763422	0.0
6	4.8523855783218706E-5	0.0	0.0	0.7478981891867499	0.0
7	4.8523855783218706E-5	0.0	0.0	0.9092885335217353	0.0
8	4.8523855783218706E-5	0.0	0.0	1.2408035162326854	0.0
9	4.8523855783218706E-5	0.0	0.0	1.3307182209989898	0.0
10	4.8523855783218706E-5	0.0	0.0	1.5163704932255846	0.0
11	9.704771156643741E-5	0.0	0.0	1.8000894779900642	0.0
12	9.704771156643741E-5	0.0	0.0	2.01500163525394	0.0
13	9.704771156643741E-5	0.0	0.0	2.0927853760744393	0.0
14	9.704771156643741E-5	0.0	0.0	2.122239356534853	0.0
15	9.704771156643741E-5	0.0	0.0	2.1748392162038623	0.0
16	1.455715673496561E-4	0.0	0.0	2.304592006568189	0.0
17	1.455715673496561E-4	0.0	0.0	2.470300974067881	0.0
18	1.455715673496561E-4	0.0	0.0	2.67245135726077	0.0
19	1.455715673496561E-4	0.0	0.0	2.8069594854918525	0.0
20	1.455715673496561E-4	0.0	0.0	2.9584509632470612	0.0
21	1.455715673496561E-4	0.0	0.0	3.154099149764999	0.0
22	1.455715673496561E-4	0.0	0.0	3.3805599847052807	0.0
23	1.455715673496561E-4	0.0	0.0	3.606778200366646	0.0
24	1.455715673496561E-4	0.0	0.0	3.7764176001847787	0.0
25	1.455715673496561E-4	0.0	0.0	3.9345568461822884	0.0
26	1.455715673496561E-4	0.0	0.0	4.080807747512909	0.0
27	1.455715673496561E-4	0.0	0.0	4.232347749123901	0.0
28	1.455715673496561E-4	0.0	0.0	4.39980357543179	0.0
29	1.455715673496561E-4	0.0	0.0	4.597247144613706	0.0
30	1.455715673496561E-4	0.0	0.0	4.816817592032771	0.0
31	1.455715673496561E-4	0.0	0.0	5.017027020994331	0.0
32	1.455715673496561E-4	0.0	0.0	5.200010481152849	0.0
33	1.455715673496561E-4	0.0	0.0	5.385614229523661	0.0
34	1.455715673496561E-4	0.0	0.0	5.584319418955942	0.0
35	1.455715673496561E-4	0.0	0.0	5.7929719988237816	0.0
36	1.455715673496561E-4	0.0	0.0	6.009533967184287	0.0
37	1.455715673496561E-4	0.0	0.0	6.233762704758541	0.0
38	1.455715673496561E-4	0.0	0.0	6.478759652608011	0.0
39	1.455715673496561E-4	0.0	0.0	6.877965414136552	0.0
40	1.455715673496561E-4	0.0	0.0	7.1376165664325555	0.0
41	1.455715673496561E-4	0.0	0.0	7.375480507481893	0.0
42	1.455715673496561E-4	0.0	0.0	7.628144224545113	0.0
43	1.455715673496561E-4	0.0	0.0	7.862271828699144	0.0
44	1.455715673496561E-4	0.0	0.0	8.106104204009817	0.0
45	1.455715673496561E-4	0.0	0.0	8.343531430357107	0.0
46	1.455715673496561E-4	0.0	0.0	8.607501205817817	0.0
47	1.455715673496561E-4	0.0	0.0	8.899081055219177	0.0
48	1.455715673496561E-4	0.0	0.0	9.167223882277243	0.0
49	1.455715673496561E-4	0.0	0.0	9.445944909896053	0.0
50	1.455715673496561E-4	0.0	0.0	9.733012040709575	0.0
51	1.455715673496561E-4	0.0	0.0	10.015517929079474	0.0
52	1.455715673496561E-4	0.0	0.0	10.349798771570066	0.0
53	1.455715673496561E-4	0.0	0.0	10.642494669654441	0.0
54	1.455715673496561E-4	0.0	0.0	10.933006994228572	0.0
55	1.455715673496561E-4	0.0	0.0	11.229827420054521	0.0
56	1.455715673496561E-4	0.0	0.0	11.5210676024654	0.0
57	1.455715673496561E-4	0.0	0.0	11.83040718308342	0.0
58	1.455715673496561E-4	0.0	0.0	12.114805501828863	0.0
59	1.455715673496561E-4	0.0	0.0	12.42681389451496	0.0
60	1.455715673496561E-4	0.0	0.0	12.750710631867944	0.0
61	1.455715673496561E-4	0.0	0.0	13.074655893076713	0.0
62	1.9409542313287483E-4	0.0	0.0	13.422377843619259	0.0
63	1.9409542313287483E-4	0.0	0.0	13.768547030776741	0.0
64	1.9409542313287483E-4	0.0	0.0	14.09943120336251	0.0
65	1.9409542313287483E-4	0.0	0.0	14.430266852092494	0.0
66	2.4261927891609353E-4	0.0	0.0	14.744555866000402	0.0
67	2.4261927891609353E-4	0.0	0.0	15.06534707658326	0.0
68	2.4261927891609353E-4	0.0	0.0	15.422142988157267	0.0
69	2.4261927891609353E-4	0.0	0.0	15.935379810776372	0.0
70	2.911431346993122E-4	0.0	0.0	16.284557476992415	0.0
71	3.396669904825309E-4	0.0	0.0	16.624661182176993	0.0
72	3.396669904825309E-4	0.0	0.0	16.958650881532886	0.0
73	3.396669904825309E-4	0.0	0.0	17.290699626657453	0.0
74	3.8819084626574965E-4	0.0	0.0	17.638081910209515	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	7630	0.0	74.91481	1
TAGCGCG	115	0.0	73.04348	1
ACGGGTA	465	0.0	72.258064	5
CGTAAGG	340	0.0	70.588234	2
TACGGGT	695	0.0	67.913666	4
TAGGGTA	2125	0.0	67.011765	5
AGACGCG	85	0.0	65.882355	1
GGGCGAT	1280	0.0	65.625	7
CTATGCG	55	9.094947E-12	65.454544	1
GCGGGTC	965	0.0	64.663216	5
GACGGGT	675	0.0	64.59259	4
TCGATAG	150	0.0	64.0	1
TATAGCG	100	0.0	64.0	1
CGGGTAG	460	0.0	63.478264	6
TGCGGGT	1340	0.0	63.283585	4
CTACGGG	380	0.0	63.157894	3
TTAGCGG	445	0.0	62.921345	2
ACGGGTG	850	0.0	62.588234	5
TATTGCG	295	0.0	62.37288	1
GTGCGGG	1285	0.0	62.25681	3
>>END_MODULE