FastQCFastQC Report
Thu 26 May 2016
SRR1547282_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547282_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2221131
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133830.6025308727850811No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAG25040.1127353586978886No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT9300.070.107535
CGTTTTT72300.069.3775941
TCGATAG2000.066.01
GTACGCG850.065.8823551
CGTAAGG5000.065.62
TAGCGGG11850.063.7974663
CGAGGGA12250.063.673474
TACGCGG1950.063.589742
CGGGATC6150.063.089436
TACGGGA6900.062.6086964
AGGGATT37000.062.1621636
GCGTAAG2850.061.7543831
TTAGGGA23200.061.3793074
TAGTCGG1050.060.9523852
TATAGCG2250.060.4444431
CGTTAGG3000.060.02
TAAGGGA25300.059.7628444
TAGGGAG24000.059.05
CGATAGG4000.059.02
TCGTTAG750.058.6666681
Produced by FastQC (version 0.11.5)